Trp-999 of β-Galactosidase (Escherichia coli) Is a Key Residue for Binding, Catalysis, and Synthesis of Allolactose, the Natural Lac Operon Inducer

Trp-999 is a key residue for the action of β-galactosidases (Escherichia coli). Several site specific substitutions (Phe, Gly, Tyr, Leu) for Trp-999 were made. Each substitution caused greatly decreased affinities for substrates and inhibitors that bind in the “shallow” mode, while the affinities of...

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