Dlg, Scrib and Lgl regulate neuroblast cell size and mitotic spindle asymmetry
Asymmetric cell division is important in generating cell diversity from bacteria to mammals. Drosophila melanogaster neuroblasts are a useful model system for investigating asymmetric cell division because they establish distinct apical–basal cortical domains, have an asymmetric mitotic spindle alig...
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| Veröffentlicht in: | Nature cell biology 2003-02, Vol.5 (2), p.166-170 |
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| creator | Albertson, Roger Doe, Chris Q. |
| description | Asymmetric cell division is important in generating cell diversity from bacteria to mammals.
Drosophila melanogaster
neuroblasts are a useful model system for investigating asymmetric cell division because they establish distinct apical–basal cortical domains, have an asymmetric mitotic spindle aligned along the apical–basal axis, and divide unequally to produce a large apical neuroblast and a small basal daughter cell (GMC)
1
,
2
. Here we show that Discs large (Dlg), Scribble (Scrib) and Lethal giant larvae (Lgl) tumour suppressor proteins regulate multiple aspects of neuroblast asymmetric cell division. Dlg/Scrib/Lgl proteins show apical cortical enrichment at prophase/metaphase, and then have a uniform cortical distribution. Mutants have defects in basal protein targeting, a reduced apical cortical domain and reduced apical spindle size. Defects in apical cell and spindle pole size result in symmetric or inverted neuroblast cell divisions. Inverted divisions correlate with the appearance of abnormally small neuroblasts and large GMCs, showing that neuroblast/GMC identity is more tightly linked to cortical determinants than cell size. We conclude that Dlg/Scrib/Lgl are important in regulating cortical polarity, cell size asymmetry and mitotic spindle asymmetry in
Drosophila
neuroblasts. |
| doi_str_mv | 10.1038/ncb922 |
| format | Article |
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Drosophila melanogaster
neuroblasts are a useful model system for investigating asymmetric cell division because they establish distinct apical–basal cortical domains, have an asymmetric mitotic spindle aligned along the apical–basal axis, and divide unequally to produce a large apical neuroblast and a small basal daughter cell (GMC)
1
,
2
. Here we show that Discs large (Dlg), Scribble (Scrib) and Lethal giant larvae (Lgl) tumour suppressor proteins regulate multiple aspects of neuroblast asymmetric cell division. Dlg/Scrib/Lgl proteins show apical cortical enrichment at prophase/metaphase, and then have a uniform cortical distribution. Mutants have defects in basal protein targeting, a reduced apical cortical domain and reduced apical spindle size. Defects in apical cell and spindle pole size result in symmetric or inverted neuroblast cell divisions. Inverted divisions correlate with the appearance of abnormally small neuroblasts and large GMCs, showing that neuroblast/GMC identity is more tightly linked to cortical determinants than cell size. We conclude that Dlg/Scrib/Lgl are important in regulating cortical polarity, cell size asymmetry and mitotic spindle asymmetry in
Drosophila
neuroblasts.</description><identifier>ISSN: 1465-7392</identifier><identifier>ISSN: 1476-4679</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/ncb922</identifier><identifier>PMID: 12545176</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Asymmetry ; Biomedical and Life Sciences ; Cancer Research ; Cell Biology ; Cell Cycle Proteins - genetics ; Cell Cycle Proteins - metabolism ; Cell division ; Cell Division - physiology ; Cell Polarity ; Cell Size ; Developmental Biology ; Drosophila melanogaster - physiology ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insects ; Kinases ; Larvae ; letter ; Life Sciences ; Localization ; Mammals ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Microscopy, Fluorescence ; Neurons ; Neurons - cytology ; Neurons - physiology ; Physiological aspects ; Proteins ; Spindle Apparatus - metabolism ; Stem Cells ; Tumor suppressor genes ; Tumor Suppressor Proteins - genetics ; Tumor Suppressor Proteins - metabolism</subject><ispartof>Nature cell biology, 2003-02, Vol.5 (2), p.166-170</ispartof><rights>Springer Nature Limited 2003</rights><rights>COPYRIGHT 2003 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Feb 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-414214e950da9d7ab7dc6933e673996a5226c5b7ecdad901b0688d6a48224f983</citedby><cites>FETCH-LOGICAL-c466t-414214e950da9d7ab7dc6933e673996a5226c5b7ecdad901b0688d6a48224f983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/ncb922$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/ncb922$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12545176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Albertson, Roger</creatorcontrib><creatorcontrib>Doe, Chris Q.</creatorcontrib><title>Dlg, Scrib and Lgl regulate neuroblast cell size and mitotic spindle asymmetry</title><title>Nature cell biology</title><addtitle>Nat Cell Biol</addtitle><addtitle>Nat Cell Biol</addtitle><description>Asymmetric cell division is important in generating cell diversity from bacteria to mammals.
Drosophila melanogaster
neuroblasts are a useful model system for investigating asymmetric cell division because they establish distinct apical–basal cortical domains, have an asymmetric mitotic spindle aligned along the apical–basal axis, and divide unequally to produce a large apical neuroblast and a small basal daughter cell (GMC)
1
,
2
. Here we show that Discs large (Dlg), Scribble (Scrib) and Lethal giant larvae (Lgl) tumour suppressor proteins regulate multiple aspects of neuroblast asymmetric cell division. Dlg/Scrib/Lgl proteins show apical cortical enrichment at prophase/metaphase, and then have a uniform cortical distribution. Mutants have defects in basal protein targeting, a reduced apical cortical domain and reduced apical spindle size. Defects in apical cell and spindle pole size result in symmetric or inverted neuroblast cell divisions. Inverted divisions correlate with the appearance of abnormally small neuroblasts and large GMCs, showing that neuroblast/GMC identity is more tightly linked to cortical determinants than cell size. We conclude that Dlg/Scrib/Lgl are important in regulating cortical polarity, cell size asymmetry and mitotic spindle asymmetry in
Drosophila
neuroblasts.</description><subject>Animals</subject><subject>Asymmetry</subject><subject>Biomedical and Life Sciences</subject><subject>Cancer Research</subject><subject>Cell Biology</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell division</subject><subject>Cell Division - physiology</subject><subject>Cell Polarity</subject><subject>Cell Size</subject><subject>Developmental Biology</subject><subject>Drosophila melanogaster - physiology</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Insects</subject><subject>Kinases</subject><subject>Larvae</subject><subject>letter</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Mammals</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Microscopy, Fluorescence</subject><subject>Neurons</subject><subject>Neurons - cytology</subject><subject>Neurons - physiology</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Spindle Apparatus - metabolism</subject><subject>Stem Cells</subject><subject>Tumor suppressor genes</subject><subject>Tumor Suppressor Proteins - genetics</subject><subject>Tumor Suppressor Proteins - metabolism</subject><issn>1465-7392</issn><issn>1476-4679</issn><issn>1476-4679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkmtrFTEQhhdRbK36E2RRUAS35ra5fCz1VjgoWP0cssnskpLdPU2y4PHXm_UcKKcIkg8TZp55mZeZqnqO0TlGVL6fbKcIeVCdYiZ4w7hQD9c_bxtBFTmpnqR0gxBmDInH1QkmLWux4KfV1w9heFdf2-i72kyu3gyhjjAswWSoJ1ji3AWTcm0hhDr53_CXGn2es7d12vrJhZJLu3GEHHdPq0e9CQmeHeJZ9fPTxx-XX5rNt89XlxebxjLOc8MwI5iBapEzygnTCWe5ohR4mVZx0xLCbdsJsM44hXCHuJSOGyYJYb2S9Kx6vdfdxvl2gZT16NM6o5lgXpIWFCFEqfoviKVQUopV8eU98GZe4lRMaEIQ5hQrXKBXe2gwAbSf-jlHY1dFfYElJUUI8UKd_4Mqz8Ho7TxB70v-qOHtUUNhMvzKg1lS0lfX34_ZgyEb55Qi9Hob_WjiTmOk11PQ-1Mo4IuDoaUbwd1hh90X4M0eSKU0DRDvHN-T-gMK9bco</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>Albertson, Roger</creator><creator>Doe, Chris Q.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20030201</creationdate><title>Dlg, Scrib and Lgl regulate neuroblast cell size and mitotic spindle asymmetry</title><author>Albertson, Roger ; Doe, Chris Q.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-414214e950da9d7ab7dc6933e673996a5226c5b7ecdad901b0688d6a48224f983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Asymmetry</topic><topic>Biomedical and Life Sciences</topic><topic>Cancer Research</topic><topic>Cell Biology</topic><topic>Cell Cycle Proteins - 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genetics</topic><topic>Tumor Suppressor Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Albertson, Roger</creatorcontrib><creatorcontrib>Doe, Chris Q.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Nature cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Albertson, Roger</au><au>Doe, Chris Q.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dlg, Scrib and Lgl regulate neuroblast cell size and mitotic spindle asymmetry</atitle><jtitle>Nature cell biology</jtitle><stitle>Nat Cell Biol</stitle><addtitle>Nat Cell Biol</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>5</volume><issue>2</issue><spage>166</spage><epage>170</epage><pages>166-170</pages><issn>1465-7392</issn><issn>1476-4679</issn><eissn>1476-4679</eissn><abstract>Asymmetric cell division is important in generating cell diversity from bacteria to mammals.
Drosophila melanogaster
neuroblasts are a useful model system for investigating asymmetric cell division because they establish distinct apical–basal cortical domains, have an asymmetric mitotic spindle aligned along the apical–basal axis, and divide unequally to produce a large apical neuroblast and a small basal daughter cell (GMC)
1
,
2
. Here we show that Discs large (Dlg), Scribble (Scrib) and Lethal giant larvae (Lgl) tumour suppressor proteins regulate multiple aspects of neuroblast asymmetric cell division. Dlg/Scrib/Lgl proteins show apical cortical enrichment at prophase/metaphase, and then have a uniform cortical distribution. Mutants have defects in basal protein targeting, a reduced apical cortical domain and reduced apical spindle size. Defects in apical cell and spindle pole size result in symmetric or inverted neuroblast cell divisions. Inverted divisions correlate with the appearance of abnormally small neuroblasts and large GMCs, showing that neuroblast/GMC identity is more tightly linked to cortical determinants than cell size. We conclude that Dlg/Scrib/Lgl are important in regulating cortical polarity, cell size asymmetry and mitotic spindle asymmetry in
Drosophila
neuroblasts.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>12545176</pmid><doi>10.1038/ncb922</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | Animals Asymmetry Biomedical and Life Sciences Cancer Research Cell Biology Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Cell division Cell Division - physiology Cell Polarity Cell Size Developmental Biology Drosophila melanogaster - physiology Drosophila Proteins - genetics Drosophila Proteins - metabolism Insect Proteins - genetics Insect Proteins - metabolism Insects Kinases Larvae letter Life Sciences Localization Mammals Membrane Proteins - genetics Membrane Proteins - metabolism Microscopy, Fluorescence Neurons Neurons - cytology Neurons - physiology Physiological aspects Proteins Spindle Apparatus - metabolism Stem Cells Tumor suppressor genes Tumor Suppressor Proteins - genetics Tumor Suppressor Proteins - metabolism |
| title | Dlg, Scrib and Lgl regulate neuroblast cell size and mitotic spindle asymmetry |
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