Melatonin increases oestradiol-induced bone formation in ovariectomized rats
: To assess the effect of melatonin on bone metabolism in ovariectomized rats, receiving oestradiol therapy or not, melatonin was administered in the drinking water (25 μg/mL water) and oestradiol (10 μg/kg body weight) or vehicle was given subcutaneously 5 days/week for up to 60 days after surgery....
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| Veröffentlicht in: | Journal of pineal research 2003-03, Vol.34 (2), p.143-151 |
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| creator | Ladizesky, Marta G. Boggio, Verónica Albornoz, Liliana E. Castrillón, Patricia O. Mautalen, Carlos Cardinali, Daniel P. |
| description | : To assess the effect of melatonin on bone metabolism in ovariectomized rats, receiving oestradiol therapy or not, melatonin was administered in the drinking water (25 μg/mL water) and oestradiol (10 μg/kg body weight) or vehicle was given subcutaneously 5 days/week for up to 60 days after surgery. Urinary deoxypyridinoline (a marker of bone resorption) and circulating levels of bone alkaline phosphatase activity (a marker of bone formation), as well as serum calcium and phosphorus levels, were measured every 15 days. Bone area (BA), bone mineral content (BMC), bone mineral density (BMD) and total body fat (expressed as 100 g body weight) were measured by dual‐energy X‐ray absorptiometry at the end of the experiment. Body weight and total body fat were augmented after ovariectomy, and decreased after melatonin or oestradiol treatment. The effect of melatonin on body weight was seen in sham‐operated rats only. Ovariectomy augmented, and melatonin or oestradiol lowered, urinary deoxypyridinoline excretion. This effect of melatonin and oestradiol was seen mainly in ovariectomized rats. The efficacy of oestradiol to counteract ovariectomy‐induced bone resorption was increased by melatonin. Melatonin or oestradiol lowered serum bone alkaline phosphatase activity. Melatonin inhibition was seen mainly on the increase of bone alkaline phosphatase activity that followed ovariectomy. Serum phosphorus levels decreased after melatonin administration and were augmented after oestradiol injection; overall, melatonin impaired the increase of serum phosphorus caused by oestradiol. Ovariectomy decreased, and oestradiol increased, serum calcium levels while melatonin augmented serum calcium in sham‐operated rats only. On day 60 after surgery, BMD and content decreased after ovariectomy and were increased after oestradiol injection. Melatonin augmented BA of spine and BMC of whole of the skeleton and tibia. The highest values observed were those of rats treated concurrently with oestradiol and melatonin. The present results indicate that: (i) melatonin treatment restrained bone remodelling after ovariectomy; (ii) the effect of melatonin required adequate concentrations of oestradiol; (iii) melatonin augmented oestradiol effects on bone in ovariectomized rats; (iv) a counter‐regulation by melatonin of the increase in body fat caused by ovariectomy was uncovered. The melatonin doses employed were pharmacological in terms of circulating melatonin levels but not necessarily for |
| doi_str_mv | 10.1034/j.1600-079X.2003.00021.x |
| format | Article |
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Urinary deoxypyridinoline (a marker of bone resorption) and circulating levels of bone alkaline phosphatase activity (a marker of bone formation), as well as serum calcium and phosphorus levels, were measured every 15 days. Bone area (BA), bone mineral content (BMC), bone mineral density (BMD) and total body fat (expressed as 100 g body weight) were measured by dual‐energy X‐ray absorptiometry at the end of the experiment. Body weight and total body fat were augmented after ovariectomy, and decreased after melatonin or oestradiol treatment. The effect of melatonin on body weight was seen in sham‐operated rats only. Ovariectomy augmented, and melatonin or oestradiol lowered, urinary deoxypyridinoline excretion. This effect of melatonin and oestradiol was seen mainly in ovariectomized rats. The efficacy of oestradiol to counteract ovariectomy‐induced bone resorption was increased by melatonin. Melatonin or oestradiol lowered serum bone alkaline phosphatase activity. Melatonin inhibition was seen mainly on the increase of bone alkaline phosphatase activity that followed ovariectomy. Serum phosphorus levels decreased after melatonin administration and were augmented after oestradiol injection; overall, melatonin impaired the increase of serum phosphorus caused by oestradiol. Ovariectomy decreased, and oestradiol increased, serum calcium levels while melatonin augmented serum calcium in sham‐operated rats only. On day 60 after surgery, BMD and content decreased after ovariectomy and were increased after oestradiol injection. Melatonin augmented BA of spine and BMC of whole of the skeleton and tibia. The highest values observed were those of rats treated concurrently with oestradiol and melatonin. The present results indicate that: (i) melatonin treatment restrained bone remodelling after ovariectomy; (ii) the effect of melatonin required adequate concentrations of oestradiol; (iii) melatonin augmented oestradiol effects on bone in ovariectomized rats; (iv) a counter‐regulation by melatonin of the increase in body fat caused by ovariectomy was uncovered. The melatonin doses employed were pharmacological in terms of circulating melatonin levels but not necessarily for some other fluids or tissues.</description><identifier>ISSN: 0742-3098</identifier><identifier>EISSN: 1600-079X</identifier><identifier>DOI: 10.1034/j.1600-079X.2003.00021.x</identifier><identifier>PMID: 12562506</identifier><identifier>CODEN: JPRSE9</identifier><language>eng</language><publisher>Oxford, UK: Munksgaard International Publishers</publisher><subject>Animals ; Biological and medical sciences ; body fat ; Body Weight ; Bone Development - drug effects ; bone mineral content ; bone remodelling ; Estradiol - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; melatonin ; Melatonin - pharmacology ; oestradiol ; Ovariectomy ; Rats ; Rats, Wistar ; Skeleton and joints ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Journal of pineal research, 2003-03, Vol.34 (2), p.143-151</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6341-c091575828b46d45fcb10b086d96816f5de76a8cc5b65b6dc72b87200bdd692b3</citedby><cites>FETCH-LOGICAL-c6341-c091575828b46d45fcb10b086d96816f5de76a8cc5b65b6dc72b87200bdd692b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1034%2Fj.1600-079X.2003.00021.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1034%2Fj.1600-079X.2003.00021.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14626542$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12562506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ladizesky, Marta G.</creatorcontrib><creatorcontrib>Boggio, Verónica</creatorcontrib><creatorcontrib>Albornoz, Liliana E.</creatorcontrib><creatorcontrib>Castrillón, Patricia O.</creatorcontrib><creatorcontrib>Mautalen, Carlos</creatorcontrib><creatorcontrib>Cardinali, Daniel P.</creatorcontrib><title>Melatonin increases oestradiol-induced bone formation in ovariectomized rats</title><title>Journal of pineal research</title><addtitle>J Pineal Res</addtitle><description>: To assess the effect of melatonin on bone metabolism in ovariectomized rats, receiving oestradiol therapy or not, melatonin was administered in the drinking water (25 μg/mL water) and oestradiol (10 μg/kg body weight) or vehicle was given subcutaneously 5 days/week for up to 60 days after surgery. Urinary deoxypyridinoline (a marker of bone resorption) and circulating levels of bone alkaline phosphatase activity (a marker of bone formation), as well as serum calcium and phosphorus levels, were measured every 15 days. Bone area (BA), bone mineral content (BMC), bone mineral density (BMD) and total body fat (expressed as 100 g body weight) were measured by dual‐energy X‐ray absorptiometry at the end of the experiment. Body weight and total body fat were augmented after ovariectomy, and decreased after melatonin or oestradiol treatment. The effect of melatonin on body weight was seen in sham‐operated rats only. Ovariectomy augmented, and melatonin or oestradiol lowered, urinary deoxypyridinoline excretion. This effect of melatonin and oestradiol was seen mainly in ovariectomized rats. The efficacy of oestradiol to counteract ovariectomy‐induced bone resorption was increased by melatonin. Melatonin or oestradiol lowered serum bone alkaline phosphatase activity. Melatonin inhibition was seen mainly on the increase of bone alkaline phosphatase activity that followed ovariectomy. Serum phosphorus levels decreased after melatonin administration and were augmented after oestradiol injection; overall, melatonin impaired the increase of serum phosphorus caused by oestradiol. Ovariectomy decreased, and oestradiol increased, serum calcium levels while melatonin augmented serum calcium in sham‐operated rats only. On day 60 after surgery, BMD and content decreased after ovariectomy and were increased after oestradiol injection. Melatonin augmented BA of spine and BMC of whole of the skeleton and tibia. The highest values observed were those of rats treated concurrently with oestradiol and melatonin. The present results indicate that: (i) melatonin treatment restrained bone remodelling after ovariectomy; (ii) the effect of melatonin required adequate concentrations of oestradiol; (iii) melatonin augmented oestradiol effects on bone in ovariectomized rats; (iv) a counter‐regulation by melatonin of the increase in body fat caused by ovariectomy was uncovered. The melatonin doses employed were pharmacological in terms of circulating melatonin levels but not necessarily for some other fluids or tissues.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>body fat</subject><subject>Body Weight</subject><subject>Bone Development - drug effects</subject><subject>bone mineral content</subject><subject>bone remodelling</subject><subject>Estradiol - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>melatonin</subject><subject>Melatonin - pharmacology</subject><subject>oestradiol</subject><subject>Ovariectomy</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Skeleton and joints</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>0742-3098</issn><issn>1600-079X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkNtrFDEUh4Modq3-CzIv-jbjSWZyA1-k2Iusl0Kl4kvIbSDrzKRNZnXrX2_GXdpHhUAC5_vlnPMhVGFoMLTdm02DGUANXH5rCEDbAADBze4RWt0XHqMV8I7ULUhxhJ7lvCmQEII9RUeYUEYosBVaf_SDnuMUpipMNnmdfa6iz3PSLsShDpPbWu8qEydf9TGNeg5xYav4U6fg7RzH8LsASc_5OXrS6yH7F4f7GH09fX91cl6vP59dnLxb15a1Ha4tSEw5FUSYjrmO9tZgMCCYk0xg1lPnOdPCWmpYOc5yYgQvexrnmCSmPUav9__epHi7LcOqMWTrh0FPPm6z4kRKKQT9J4iFxB0VUECxB22KOSffq5sURp3uFAa1KFcbtZhVi1m1KFd_latdib489Nia0buH4MFxAV4dAJ2tHvqkJxvyA9cxwmhHCvd2z_0Kg7_77wHUhy8X5VHi9T4e8ux393GdfijGW07V9aczBdft6fnl90t11f4Bd9Kr8w</recordid><startdate>200303</startdate><enddate>200303</enddate><creator>Ladizesky, Marta G.</creator><creator>Boggio, Verónica</creator><creator>Albornoz, Liliana E.</creator><creator>Castrillón, Patricia O.</creator><creator>Mautalen, Carlos</creator><creator>Cardinali, Daniel P.</creator><general>Munksgaard International Publishers</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>200303</creationdate><title>Melatonin increases oestradiol-induced bone formation in ovariectomized rats</title><author>Ladizesky, Marta G. ; Boggio, Verónica ; Albornoz, Liliana E. ; Castrillón, Patricia O. ; Mautalen, Carlos ; Cardinali, Daniel P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6341-c091575828b46d45fcb10b086d96816f5de76a8cc5b65b6dc72b87200bdd692b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>body fat</topic><topic>Body Weight</topic><topic>Bone Development - drug effects</topic><topic>bone mineral content</topic><topic>bone remodelling</topic><topic>Estradiol - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>melatonin</topic><topic>Melatonin - pharmacology</topic><topic>oestradiol</topic><topic>Ovariectomy</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Skeleton and joints</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ladizesky, Marta G.</creatorcontrib><creatorcontrib>Boggio, Verónica</creatorcontrib><creatorcontrib>Albornoz, Liliana E.</creatorcontrib><creatorcontrib>Castrillón, Patricia O.</creatorcontrib><creatorcontrib>Mautalen, Carlos</creatorcontrib><creatorcontrib>Cardinali, Daniel P.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pineal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ladizesky, Marta G.</au><au>Boggio, Verónica</au><au>Albornoz, Liliana E.</au><au>Castrillón, Patricia O.</au><au>Mautalen, Carlos</au><au>Cardinali, Daniel P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Melatonin increases oestradiol-induced bone formation in ovariectomized rats</atitle><jtitle>Journal of pineal research</jtitle><addtitle>J Pineal Res</addtitle><date>2003-03</date><risdate>2003</risdate><volume>34</volume><issue>2</issue><spage>143</spage><epage>151</epage><pages>143-151</pages><issn>0742-3098</issn><eissn>1600-079X</eissn><coden>JPRSE9</coden><abstract>: To assess the effect of melatonin on bone metabolism in ovariectomized rats, receiving oestradiol therapy or not, melatonin was administered in the drinking water (25 μg/mL water) and oestradiol (10 μg/kg body weight) or vehicle was given subcutaneously 5 days/week for up to 60 days after surgery. Urinary deoxypyridinoline (a marker of bone resorption) and circulating levels of bone alkaline phosphatase activity (a marker of bone formation), as well as serum calcium and phosphorus levels, were measured every 15 days. Bone area (BA), bone mineral content (BMC), bone mineral density (BMD) and total body fat (expressed as 100 g body weight) were measured by dual‐energy X‐ray absorptiometry at the end of the experiment. Body weight and total body fat were augmented after ovariectomy, and decreased after melatonin or oestradiol treatment. The effect of melatonin on body weight was seen in sham‐operated rats only. Ovariectomy augmented, and melatonin or oestradiol lowered, urinary deoxypyridinoline excretion. This effect of melatonin and oestradiol was seen mainly in ovariectomized rats. The efficacy of oestradiol to counteract ovariectomy‐induced bone resorption was increased by melatonin. Melatonin or oestradiol lowered serum bone alkaline phosphatase activity. Melatonin inhibition was seen mainly on the increase of bone alkaline phosphatase activity that followed ovariectomy. Serum phosphorus levels decreased after melatonin administration and were augmented after oestradiol injection; overall, melatonin impaired the increase of serum phosphorus caused by oestradiol. Ovariectomy decreased, and oestradiol increased, serum calcium levels while melatonin augmented serum calcium in sham‐operated rats only. On day 60 after surgery, BMD and content decreased after ovariectomy and were increased after oestradiol injection. Melatonin augmented BA of spine and BMC of whole of the skeleton and tibia. The highest values observed were those of rats treated concurrently with oestradiol and melatonin. The present results indicate that: (i) melatonin treatment restrained bone remodelling after ovariectomy; (ii) the effect of melatonin required adequate concentrations of oestradiol; (iii) melatonin augmented oestradiol effects on bone in ovariectomized rats; (iv) a counter‐regulation by melatonin of the increase in body fat caused by ovariectomy was uncovered. The melatonin doses employed were pharmacological in terms of circulating melatonin levels but not necessarily for some other fluids or tissues.</abstract><cop>Oxford, UK</cop><pub>Munksgaard International Publishers</pub><pmid>12562506</pmid><doi>10.1034/j.1600-079X.2003.00021.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences body fat Body Weight Bone Development - drug effects bone mineral content bone remodelling Estradiol - pharmacology Female Fundamental and applied biological sciences. Psychology melatonin Melatonin - pharmacology oestradiol Ovariectomy Rats Rats, Wistar Skeleton and joints Vertebrates: osteoarticular system, musculoskeletal system |
| title | Melatonin increases oestradiol-induced bone formation in ovariectomized rats |
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