Study of the polyol pathway and cell permeability changes in human lens and retinal pigment epithelium in tissue culture
The polyol pathway was investigated in primary cultures of human retinal pigment epithelial (HRPE) cells and the results were compared with those in human lens epithelial (HLE) cells cultured under similar conditions. Significant levels of galactitol were formed in HRPE cells cultured for 72 hr in a...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 1992-06, Vol.33 (7), p.2334-2339 |
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| container_title | Investigative ophthalmology & visual science |
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| creator | Reddy, VN Lin, LR Giblin, FJ Chakrapani, B Yokoyama, T |
| description | The polyol pathway was investigated in primary cultures of human retinal pigment epithelial (HRPE) cells and the results were compared with those in human lens epithelial (HLE) cells cultured under similar conditions. Significant levels of galactitol were formed in HRPE cells cultured for 72 hr in a medium containing 30 mmol/l D-galactose. Polyol accumulation was accompanied by the appearance of vacuoles as seen by transmission electron microscopy (TEM). Biochemical analysis revealed a significant depletion of cellular myoinositol, taurine, and a number of other free amino acids similar to those in HLE cells. These morphologic and biochemical changes observed in HRPE cells cultured in high galactose medium were inhibited or prevented by the inclusion of an aldose reductase inhibitor in the medium, further supporting the view that vacuole formation is due to the osmotic effect of polyol formation mediated by aldose reductase. The similarity of intracellular vacuole formation resulting from polyol accumulation and the biochemical changes in HRPE and HLE cells strongly suggests that a common mechanism is involved. |
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Significant levels of galactitol were formed in HRPE cells cultured for 72 hr in a medium containing 30 mmol/l D-galactose. Polyol accumulation was accompanied by the appearance of vacuoles as seen by transmission electron microscopy (TEM). Biochemical analysis revealed a significant depletion of cellular myoinositol, taurine, and a number of other free amino acids similar to those in HLE cells. These morphologic and biochemical changes observed in HRPE cells cultured in high galactose medium were inhibited or prevented by the inclusion of an aldose reductase inhibitor in the medium, further supporting the view that vacuole formation is due to the osmotic effect of polyol formation mediated by aldose reductase. The similarity of intracellular vacuole formation resulting from polyol accumulation and the biochemical changes in HRPE and HLE cells strongly suggests that a common mechanism is involved.</description><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>PMID: 1607245</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Adult ; Aldehyde Reductase - antagonists & inhibitors ; Biological and medical sciences ; Cell Membrane Permeability ; Cells, Cultured ; Chromatography, High Pressure Liquid ; Epithelium - metabolism ; Epithelium - ultrastructure ; Fundamental and applied biological sciences. Psychology ; Galactitol - antagonists & inhibitors ; Galactitol - metabolism ; Galactose - pharmacology ; Glutathione - metabolism ; Humans ; Inositol - metabolism ; Lens, Crystalline - metabolism ; Lens, Crystalline - ultrastructure ; Middle Aged ; Pigment Epithelium of Eye - metabolism ; Pigment Epithelium of Eye - ultrastructure ; Taurine - metabolism ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Investigative ophthalmology & visual science, 1992-06, Vol.33 (7), p.2334-2339</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5574579$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1607245$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reddy, VN</creatorcontrib><creatorcontrib>Lin, LR</creatorcontrib><creatorcontrib>Giblin, FJ</creatorcontrib><creatorcontrib>Chakrapani, B</creatorcontrib><creatorcontrib>Yokoyama, T</creatorcontrib><title>Study of the polyol pathway and cell permeability changes in human lens and retinal pigment epithelium in tissue culture</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>The polyol pathway was investigated in primary cultures of human retinal pigment epithelial (HRPE) cells and the results were compared with those in human lens epithelial (HLE) cells cultured under similar conditions. Significant levels of galactitol were formed in HRPE cells cultured for 72 hr in a medium containing 30 mmol/l D-galactose. Polyol accumulation was accompanied by the appearance of vacuoles as seen by transmission electron microscopy (TEM). Biochemical analysis revealed a significant depletion of cellular myoinositol, taurine, and a number of other free amino acids similar to those in HLE cells. These morphologic and biochemical changes observed in HRPE cells cultured in high galactose medium were inhibited or prevented by the inclusion of an aldose reductase inhibitor in the medium, further supporting the view that vacuole formation is due to the osmotic effect of polyol formation mediated by aldose reductase. The similarity of intracellular vacuole formation resulting from polyol accumulation and the biochemical changes in HRPE and HLE cells strongly suggests that a common mechanism is involved.</description><subject>Adult</subject><subject>Aldehyde Reductase - antagonists & inhibitors</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane Permeability</subject><subject>Cells, Cultured</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Epithelium - metabolism</subject><subject>Epithelium - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galactitol - antagonists & inhibitors</subject><subject>Galactitol - metabolism</subject><subject>Galactose - pharmacology</subject><subject>Glutathione - metabolism</subject><subject>Humans</subject><subject>Inositol - metabolism</subject><subject>Lens, Crystalline - metabolism</subject><subject>Lens, Crystalline - ultrastructure</subject><subject>Middle Aged</subject><subject>Pigment Epithelium of Eye - metabolism</subject><subject>Pigment Epithelium of Eye - ultrastructure</subject><subject>Taurine - metabolism</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1r3DAQhk1p2G43_QkFHUJvBn3LPoYlaQILPbQ9m7E9XqvIsmNJOP739TZLehqY9-EZ5v2Q7ZlSPFemEB-zPWVS51RS-Sn7HMIfSjljnO6yHdPUcKn22evPmNqVjB2JPZJpdOvoyASxX2Al4FvSoNsWOA8ItXU2rqTpwZ8xEOtJnwbwxKEP_9gZo_Ww4fY8oI8EJ7tZnU3DBY42hISkSS6mGW-zmw5cwC_Xech-Pz78Oj7lpx_fn4_3p7znWsdcdW0ppeCqZi2tleGm4K2QnVY1SICm06g1a4GbTrdouqZAKtCgKkXNSl6IQ_btzTvN40vCEKvBhstT4HFMoTK8LAujLuDXK5jqAdtqmu0A81pdq9ryu2sOoQHXzeAbG94xpYxUpvx_r7fnfrEzVmEA5zYpq5ZlEaIyFRdCir_iYoIx</recordid><startdate>19920601</startdate><enddate>19920601</enddate><creator>Reddy, VN</creator><creator>Lin, LR</creator><creator>Giblin, FJ</creator><creator>Chakrapani, B</creator><creator>Yokoyama, T</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19920601</creationdate><title>Study of the polyol pathway and cell permeability changes in human lens and retinal pigment epithelium in tissue culture</title><author>Reddy, VN ; Lin, LR ; Giblin, FJ ; Chakrapani, B ; Yokoyama, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h266t-5fd944325b1d0b572782d34f65ba4aacf6e661da27f6de7fc8e03e7e593b19283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adult</topic><topic>Aldehyde Reductase - antagonists & inhibitors</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane Permeability</topic><topic>Cells, Cultured</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Epithelium - metabolism</topic><topic>Epithelium - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galactitol - antagonists & inhibitors</topic><topic>Galactitol - metabolism</topic><topic>Galactose - pharmacology</topic><topic>Glutathione - metabolism</topic><topic>Humans</topic><topic>Inositol - metabolism</topic><topic>Lens, Crystalline - metabolism</topic><topic>Lens, Crystalline - ultrastructure</topic><topic>Middle Aged</topic><topic>Pigment Epithelium of Eye - metabolism</topic><topic>Pigment Epithelium of Eye - ultrastructure</topic><topic>Taurine - metabolism</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reddy, VN</creatorcontrib><creatorcontrib>Lin, LR</creatorcontrib><creatorcontrib>Giblin, FJ</creatorcontrib><creatorcontrib>Chakrapani, B</creatorcontrib><creatorcontrib>Yokoyama, T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reddy, VN</au><au>Lin, LR</au><au>Giblin, FJ</au><au>Chakrapani, B</au><au>Yokoyama, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of the polyol pathway and cell permeability changes in human lens and retinal pigment epithelium in tissue culture</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>1992-06-01</date><risdate>1992</risdate><volume>33</volume><issue>7</issue><spage>2334</spage><epage>2339</epage><pages>2334-2339</pages><issn>0146-0404</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>The polyol pathway was investigated in primary cultures of human retinal pigment epithelial (HRPE) cells and the results were compared with those in human lens epithelial (HLE) cells cultured under similar conditions. Significant levels of galactitol were formed in HRPE cells cultured for 72 hr in a medium containing 30 mmol/l D-galactose. Polyol accumulation was accompanied by the appearance of vacuoles as seen by transmission electron microscopy (TEM). Biochemical analysis revealed a significant depletion of cellular myoinositol, taurine, and a number of other free amino acids similar to those in HLE cells. These morphologic and biochemical changes observed in HRPE cells cultured in high galactose medium were inhibited or prevented by the inclusion of an aldose reductase inhibitor in the medium, further supporting the view that vacuole formation is due to the osmotic effect of polyol formation mediated by aldose reductase. The similarity of intracellular vacuole formation resulting from polyol accumulation and the biochemical changes in HRPE and HLE cells strongly suggests that a common mechanism is involved.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>1607245</pmid><tpages>6</tpages></addata></record> |
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| ispartof | Investigative ophthalmology & visual science, 1992-06, Vol.33 (7), p.2334-2339 |
| issn | 0146-0404 1552-5783 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Aldehyde Reductase - antagonists & inhibitors Biological and medical sciences Cell Membrane Permeability Cells, Cultured Chromatography, High Pressure Liquid Epithelium - metabolism Epithelium - ultrastructure Fundamental and applied biological sciences. Psychology Galactitol - antagonists & inhibitors Galactitol - metabolism Galactose - pharmacology Glutathione - metabolism Humans Inositol - metabolism Lens, Crystalline - metabolism Lens, Crystalline - ultrastructure Middle Aged Pigment Epithelium of Eye - metabolism Pigment Epithelium of Eye - ultrastructure Taurine - metabolism Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Study of the polyol pathway and cell permeability changes in human lens and retinal pigment epithelium in tissue culture |
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