Microfluidic devices for DNA sequencing: sample preparation and electrophoretic analysis
Modern DNA sequencing ‘factories’ have revolutionized biology by completing the human genome sequence, but in the race to completion we are left with inefficient, cumbersome, and costly macroscale processes and supporting facilities. During the same period, microfabricated DNA sequencing, sample pro...
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| Veröffentlicht in: | Current opinion in biotechnology 2003-02, Vol.14 (1), p.42-50 |
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| creator | Paegel, Brian M Blazej, Robert G Mathies, Richard A |
| description | Modern DNA sequencing ‘factories’ have revolutionized biology by completing the human genome sequence, but in the race to completion we are left with inefficient, cumbersome, and costly macroscale processes and supporting facilities. During the same period, microfabricated DNA sequencing, sample processing and analysis devices have advanced rapidly toward the goal of a ‘sequencing lab-on-a-chip’. Integrated microfluidic processing dramatically reduces analysis time and reagent consumption, and eliminates costly and unreliable macroscale robotics and laboratory apparatus. A microfabricated device for high-throughput DNA sequencing that couples clone isolation, template amplification, Sanger extension, purification, and electrophoretic analysis in a single microfluidic circuit is now attainable. |
| doi_str_mv | 10.1016/S0958-1669(02)00004-6 |
| format | Article |
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| ispartof | Current opinion in biotechnology, 2003-02, Vol.14 (1), p.42-50 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Base Sequence DNA - chemistry DNA - genetics DNA - isolation & purification Electrophoresis - instrumentation Electrophoresis - methods Equipment Design Flow Cytometry - instrumentation Flow Cytometry - methods Microchemistry - instrumentation Microchemistry - methods Molecular Sequence Data Nanotechnology - instrumentation Nanotechnology - methods Oligonucleotide Array Sequence Analysis - instrumentation Oligonucleotide Array Sequence Analysis - methods Polymerase Chain Reaction Rheology - instrumentation Rheology - methods Robotics - instrumentation Robotics - methods Sequence Analysis, DNA - instrumentation Sequence Analysis, DNA - methods Specimen Handling - instrumentation Specimen Handling - methods Systems Integration |
| title | Microfluidic devices for DNA sequencing: sample preparation and electrophoretic analysis |
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