Induction of CD-RAP mRNA during periosteal chondrogenesis
Induction of chondrogenesis and maintenance of the chondrocyte phenotype are critical events for autologous periosteal transplantation, which is a viable approach for cartilage repair. Cartilage-derived retinoic acid-sensitive protein (CD-RAP) is a recently discovered protein that is mainly produced...
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| Veröffentlicht in: | Journal of orthopaedic research 2003-03, Vol.21 (2), p.296-304 |
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| creator | Sanyal, Arunik Clemens, Victoria Fitzsimmons, James S. Reinholz, Gregory G. Sarkar, Gobinda Mukherjee, Nilay O’Driscoll, Shawn W. |
| description | Induction of chondrogenesis and maintenance of the chondrocyte phenotype are critical events for autologous periosteal transplantation, which is a viable approach for cartilage repair. Cartilage-derived retinoic acid-sensitive protein (CD-RAP) is a recently discovered protein that is mainly produced in cartilage. During development,
CD-RAP expression starts at the beginning of chondrogenesis and continues throughout cartilage maturation. In order to investigate the involvement of CD-RAP during periosteal chondrogenesis we have determined the nucleotide sequence of the rabbit CD-RAP mRNA and utilized this information to evaluate the temporal and spatial expression pattern of
CD-RAP at the mRNA level during chondrogenesis. When the periosteal explants were cultured under chondrogenic conditions, the expression of
CD-RAP was induced, as shown by a 40-fold increase in CD-RAP mRNA between days 7 and 10. The temporal expression pattern of
CD-RAP closely mimicked that of collagen type IIB mRNA. Also, the CD-RAP mRNA was localized to the matrix forming chondrocytes in the cambium layer of the periosteum by in situ hybridization as indicated by colocalization with collagen type II mRNA and positive safranin O staining. These data suggest a regulatory role of CD-RAP in periosteal chondrogenesis, which is potentially important for both cartilage repair and fracture healing via callus formation. |
| doi_str_mv | 10.1016/S0736-0266(02)00158-4 |
| format | Article |
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CD-RAP expression starts at the beginning of chondrogenesis and continues throughout cartilage maturation. In order to investigate the involvement of CD-RAP during periosteal chondrogenesis we have determined the nucleotide sequence of the rabbit CD-RAP mRNA and utilized this information to evaluate the temporal and spatial expression pattern of
CD-RAP at the mRNA level during chondrogenesis. When the periosteal explants were cultured under chondrogenic conditions, the expression of
CD-RAP was induced, as shown by a 40-fold increase in CD-RAP mRNA between days 7 and 10. The temporal expression pattern of
CD-RAP closely mimicked that of collagen type IIB mRNA. Also, the CD-RAP mRNA was localized to the matrix forming chondrocytes in the cambium layer of the periosteum by in situ hybridization as indicated by colocalization with collagen type II mRNA and positive safranin O staining. These data suggest a regulatory role of CD-RAP in periosteal chondrogenesis, which is potentially important for both cartilage repair and fracture healing via callus formation.</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1016/S0736-0266(02)00158-4</identifier><identifier>PMID: 12568962</identifier><identifier>CODEN: JOREDR</identifier><language>eng</language><publisher>Hoboken: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Cartilage biology ; Cartilage-derived retinoic acid-sensitive protein ; Cattle ; Cells, Cultured ; Chondrocytes - cytology ; Chondrocytes - metabolism ; Chondrogenesis ; Chondrogenesis - genetics ; DNA Primers - chemistry ; DNA, Complementary - analysis ; Extracellular Matrix Proteins ; Gene Expression ; Humans ; In Situ Hybridization ; Mice ; Molecular Sequence Data ; Neoplasm Proteins ; Periosteum ; Periosteum - cytology ; Periosteum - metabolism ; Proteins - genetics ; Proteins - metabolism ; Rabbits ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; RT-PCR ; Sequence Alignment ; Species Specificity</subject><ispartof>Journal of orthopaedic research, 2003-03, Vol.21 (2), p.296-304</ispartof><rights>2003 Orthopaedic Research Society</rights><rights>Copyright © 2003 Orthopaedic Research Society</rights><rights>Copyright Journal of Bone and Joint Surgery, Inc. Mar 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4626-582e4a4ddb686914f19bdee6a5f831b49250f09e8c2f91c52e38b8d8f21f460d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0736-0266%2802%2900158-4$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0736-0266%2802%2900158-4$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12568962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sanyal, Arunik</creatorcontrib><creatorcontrib>Clemens, Victoria</creatorcontrib><creatorcontrib>Fitzsimmons, James S.</creatorcontrib><creatorcontrib>Reinholz, Gregory G.</creatorcontrib><creatorcontrib>Sarkar, Gobinda</creatorcontrib><creatorcontrib>Mukherjee, Nilay</creatorcontrib><creatorcontrib>O’Driscoll, Shawn W.</creatorcontrib><title>Induction of CD-RAP mRNA during periosteal chondrogenesis</title><title>Journal of orthopaedic research</title><addtitle>J. Orthop. Res</addtitle><description>Induction of chondrogenesis and maintenance of the chondrocyte phenotype are critical events for autologous periosteal transplantation, which is a viable approach for cartilage repair. Cartilage-derived retinoic acid-sensitive protein (CD-RAP) is a recently discovered protein that is mainly produced in cartilage. During development,
CD-RAP expression starts at the beginning of chondrogenesis and continues throughout cartilage maturation. In order to investigate the involvement of CD-RAP during periosteal chondrogenesis we have determined the nucleotide sequence of the rabbit CD-RAP mRNA and utilized this information to evaluate the temporal and spatial expression pattern of
CD-RAP at the mRNA level during chondrogenesis. When the periosteal explants were cultured under chondrogenic conditions, the expression of
CD-RAP was induced, as shown by a 40-fold increase in CD-RAP mRNA between days 7 and 10. The temporal expression pattern of
CD-RAP closely mimicked that of collagen type IIB mRNA. Also, the CD-RAP mRNA was localized to the matrix forming chondrocytes in the cambium layer of the periosteum by in situ hybridization as indicated by colocalization with collagen type II mRNA and positive safranin O staining. These data suggest a regulatory role of CD-RAP in periosteal chondrogenesis, which is potentially important for both cartilage repair and fracture healing via callus formation.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cartilage biology</subject><subject>Cartilage-derived retinoic acid-sensitive protein</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - metabolism</subject><subject>Chondrogenesis</subject><subject>Chondrogenesis - genetics</subject><subject>DNA Primers - chemistry</subject><subject>DNA, Complementary - analysis</subject><subject>Extracellular Matrix Proteins</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Neoplasm Proteins</subject><subject>Periosteum</subject><subject>Periosteum - cytology</subject><subject>Periosteum - metabolism</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Rabbits</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RT-PCR</subject><subject>Sequence Alignment</subject><subject>Species Specificity</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1vEzEQhi0EoqHwE0ArDggOCx6v7bVPEAUIhdKi8NHerI0927ps1sHOAv33ON2oSFyKZI0vz7zSOw8hD4E-BwryxWdaV7KkTMqnlD2jFIQq-S0yASF4KVh9eptMrpE9ci-lC0ppDUzdJXvAhFRasgnRB70b7MaHvghtMXtdLqafitXiaFq4Ifr-rFhj9CFtsOkKex56F8MZ9ph8uk_utE2X8MHu3ydf3775MntXHh7PD2bTw9JyyWQpFEPecOeWUkkNvAW9dIiyEa2qYMk1E7SlGpVlrQYrGFZqqZxqGbRcUlftkydj7jqGHwOmjVn5ZLHrmh7DkEzNtK5rkDeCoDRoIesMPv4HvAhD7HMJwyoBAAogQ2KEbAwpRWzNOvpVEy8NULM1YK4MmO158zBXBgzPe4924cNyhe7v1u7kGXg1Ar98h5f_l2reHy8AKGWQ37ZpOUb4LOb3dUQTv5tcrxbm5Ghu2OnJx2-cz82HzL8cecyefnqMJlmPvUXnI9qNccHf0OoP9nq0Gg</recordid><startdate>200303</startdate><enddate>200303</enddate><creator>Sanyal, Arunik</creator><creator>Clemens, Victoria</creator><creator>Fitzsimmons, James S.</creator><creator>Reinholz, Gregory G.</creator><creator>Sarkar, Gobinda</creator><creator>Mukherjee, Nilay</creator><creator>O’Driscoll, Shawn W.</creator><general>Elsevier Ltd</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>200303</creationdate><title>Induction of CD-RAP mRNA during periosteal chondrogenesis</title><author>Sanyal, Arunik ; 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Orthop. Res</addtitle><date>2003-03</date><risdate>2003</risdate><volume>21</volume><issue>2</issue><spage>296</spage><epage>304</epage><pages>296-304</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><coden>JOREDR</coden><abstract>Induction of chondrogenesis and maintenance of the chondrocyte phenotype are critical events for autologous periosteal transplantation, which is a viable approach for cartilage repair. Cartilage-derived retinoic acid-sensitive protein (CD-RAP) is a recently discovered protein that is mainly produced in cartilage. During development,
CD-RAP expression starts at the beginning of chondrogenesis and continues throughout cartilage maturation. In order to investigate the involvement of CD-RAP during periosteal chondrogenesis we have determined the nucleotide sequence of the rabbit CD-RAP mRNA and utilized this information to evaluate the temporal and spatial expression pattern of
CD-RAP at the mRNA level during chondrogenesis. When the periosteal explants were cultured under chondrogenic conditions, the expression of
CD-RAP was induced, as shown by a 40-fold increase in CD-RAP mRNA between days 7 and 10. The temporal expression pattern of
CD-RAP closely mimicked that of collagen type IIB mRNA. Also, the CD-RAP mRNA was localized to the matrix forming chondrocytes in the cambium layer of the periosteum by in situ hybridization as indicated by colocalization with collagen type II mRNA and positive safranin O staining. These data suggest a regulatory role of CD-RAP in periosteal chondrogenesis, which is potentially important for both cartilage repair and fracture healing via callus formation.</abstract><cop>Hoboken</cop><pub>Elsevier Ltd</pub><pmid>12568962</pmid><doi>10.1016/S0736-0266(02)00158-4</doi><tpages>9</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Base Sequence Cartilage biology Cartilage-derived retinoic acid-sensitive protein Cattle Cells, Cultured Chondrocytes - cytology Chondrocytes - metabolism Chondrogenesis Chondrogenesis - genetics DNA Primers - chemistry DNA, Complementary - analysis Extracellular Matrix Proteins Gene Expression Humans In Situ Hybridization Mice Molecular Sequence Data Neoplasm Proteins Periosteum Periosteum - cytology Periosteum - metabolism Proteins - genetics Proteins - metabolism Rabbits Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RT-PCR Sequence Alignment Species Specificity |
| title | Induction of CD-RAP mRNA during periosteal chondrogenesis |
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