The signal flow and motor response controling chemotaxis of sea urchin sperm
The signalling pathway and the behavioural strategy underlying chemotaxis of sperm are poorly understood. We have studied the cellular events and motor responses that mediate chemotaxis of sperm from the sea urchin Arbacia punctulata. Here we show that resact, a chemoattractant peptide, initiates a...
Gespeichert in:
| Veröffentlicht in: | Nature cell biology 2003-02, Vol.5 (2), p.109-117 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 117 |
|---|---|
| container_issue | 2 |
| container_start_page | 109 |
| container_title | Nature cell biology |
| container_volume | 5 |
| creator | Kaupp, U. Benjamin Solzin, Johannes Hildebrand, Eilo Brown, Joel E Helbig, Annika Hagen, Volker Beyermann, Michael Pampaloni, Francesco Weyand, Ingo |
| description | The signalling pathway and the behavioural strategy underlying chemotaxis of sperm are poorly understood. We have studied the cellular events and motor responses that mediate chemotaxis of sperm from the sea urchin Arbacia punctulata. Here we show that resact, a chemoattractant peptide, initiates a rapid and transient rise in the concentration of cyclic GMP, followed by a transient influx of Ca2+. The binding of a single resact molecule elicits a Ca2+ response, and 50-100 bound molecules saturate the response. The ability to register single molecules is reminiscent of the single-photon sensitivity of rod photoreceptors. Both resact and cyclic nucleotides cause a turn or brief tumbling in the swimming path of sperm. We conclude that a cGMP-mediated increase in the Ca2+ concentration induces the primary motor response of sperm to the chemoattractant. |
| doi_str_mv | 10.1038/ncb915 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_72996284</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A183287799</galeid><sourcerecordid>A183287799</sourcerecordid><originalsourceid>FETCH-LOGICAL-c521t-5496fccf082273bbbf8dce94ce2ffd2ef45690005c21d8505b9cca3c61fa60e3</originalsourceid><addsrcrecordid>eNqF0t2L1DAQAPAgiveh_gUiQUHwoWeSNl-Px-HpwYKg-x7SdLKbo03WpOXO_96WLcqKIHlISH4zMJNB6BUlV5TU6mN0rab8CTqnjRRVI6R-upwFr2St2Rm6KOWeENo0RD5HZ5RxUTMpztFmuwdcwi7aHvs-PWAbOzykMWWcoRxSLIBdimNOfYg77PYwP9rHUHDyuIDFU3b7EHE5QB5eoGfe9gVervsl2t5-2t58qTZfP9_dXG8qxxkdK95o4Z3zRDEm67Ztveoc6MYB875j4BsuNCGEO0Y7xQlvtXO2doJ6KwjUl-j9Me0hpx8TlNEMoTjoexshTcVIprVgqvkvpEpxxvUC3_4F79OU554UwxiholGMzOjdEe1sDyZEn8Zs3ZLRXFNVMyWl1rO6-oeaVwdDmDsJPsz3JwEfTgKWbsPjuLNTKebu-7dTuxbkciolgzeHHAabfxpKzDIH5jgHM3yzFjS1A3R_2PrxM3h9BNGOU4bfYI3_BUUvtPM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>220164820</pqid></control><display><type>article</type><title>The signal flow and motor response controling chemotaxis of sea urchin sperm</title><source>MEDLINE</source><source>Nature Journals Online</source><source>Alma/SFX Local Collection</source><creator>Kaupp, U. Benjamin ; Solzin, Johannes ; Hildebrand, Eilo ; Brown, Joel E ; Helbig, Annika ; Hagen, Volker ; Beyermann, Michael ; Pampaloni, Francesco ; Weyand, Ingo</creator><creatorcontrib>Kaupp, U. Benjamin ; Solzin, Johannes ; Hildebrand, Eilo ; Brown, Joel E ; Helbig, Annika ; Hagen, Volker ; Beyermann, Michael ; Pampaloni, Francesco ; Weyand, Ingo</creatorcontrib><description>The signalling pathway and the behavioural strategy underlying chemotaxis of sperm are poorly understood. We have studied the cellular events and motor responses that mediate chemotaxis of sperm from the sea urchin Arbacia punctulata. Here we show that resact, a chemoattractant peptide, initiates a rapid and transient rise in the concentration of cyclic GMP, followed by a transient influx of Ca2+. The binding of a single resact molecule elicits a Ca2+ response, and 50-100 bound molecules saturate the response. The ability to register single molecules is reminiscent of the single-photon sensitivity of rod photoreceptors. Both resact and cyclic nucleotides cause a turn or brief tumbling in the swimming path of sperm. We conclude that a cGMP-mediated increase in the Ca2+ concentration induces the primary motor response of sperm to the chemoattractant.</description><identifier>ISSN: 1465-7392</identifier><identifier>ISSN: 1476-4679</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/ncb915</identifier><identifier>PMID: 12563276</identifier><language>eng</language><publisher>England: Nature Publishing Group</publisher><subject>Animals ; Arbacia punctulata ; Calcium - metabolism ; Calcium ions ; Chemotaxis ; Chemotaxis - physiology ; Cyclic AMP - analogs & derivatives ; Cyclic AMP - metabolism ; Cyclic GMP - analogs & derivatives ; Cyclic GMP - metabolism ; Egg Proteins - metabolism ; Guanylate Cyclase ; Male ; Marine ; Molecular Structure ; Peptides ; Photoreception ; Physiological aspects ; Protein Binding ; Receptors, Cell Surface - metabolism ; Sea Urchins - physiology ; Signal Transduction - physiology ; Sperm ; Sperm Motility ; Spermatozoa ; Spermatozoa - cytology ; Spermatozoa - metabolism ; Spermatozoa - physiology ; Swimming</subject><ispartof>Nature cell biology, 2003-02, Vol.5 (2), p.109-117</ispartof><rights>COPYRIGHT 2003 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Feb 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-5496fccf082273bbbf8dce94ce2ffd2ef45690005c21d8505b9cca3c61fa60e3</citedby><cites>FETCH-LOGICAL-c521t-5496fccf082273bbbf8dce94ce2ffd2ef45690005c21d8505b9cca3c61fa60e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,2731,27933,27934</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12563276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaupp, U. Benjamin</creatorcontrib><creatorcontrib>Solzin, Johannes</creatorcontrib><creatorcontrib>Hildebrand, Eilo</creatorcontrib><creatorcontrib>Brown, Joel E</creatorcontrib><creatorcontrib>Helbig, Annika</creatorcontrib><creatorcontrib>Hagen, Volker</creatorcontrib><creatorcontrib>Beyermann, Michael</creatorcontrib><creatorcontrib>Pampaloni, Francesco</creatorcontrib><creatorcontrib>Weyand, Ingo</creatorcontrib><title>The signal flow and motor response controling chemotaxis of sea urchin sperm</title><title>Nature cell biology</title><addtitle>Nat Cell Biol</addtitle><description>The signalling pathway and the behavioural strategy underlying chemotaxis of sperm are poorly understood. We have studied the cellular events and motor responses that mediate chemotaxis of sperm from the sea urchin Arbacia punctulata. Here we show that resact, a chemoattractant peptide, initiates a rapid and transient rise in the concentration of cyclic GMP, followed by a transient influx of Ca2+. The binding of a single resact molecule elicits a Ca2+ response, and 50-100 bound molecules saturate the response. The ability to register single molecules is reminiscent of the single-photon sensitivity of rod photoreceptors. Both resact and cyclic nucleotides cause a turn or brief tumbling in the swimming path of sperm. We conclude that a cGMP-mediated increase in the Ca2+ concentration induces the primary motor response of sperm to the chemoattractant.</description><subject>Animals</subject><subject>Arbacia punctulata</subject><subject>Calcium - metabolism</subject><subject>Calcium ions</subject><subject>Chemotaxis</subject><subject>Chemotaxis - physiology</subject><subject>Cyclic AMP - analogs & derivatives</subject><subject>Cyclic AMP - metabolism</subject><subject>Cyclic GMP - analogs & derivatives</subject><subject>Cyclic GMP - metabolism</subject><subject>Egg Proteins - metabolism</subject><subject>Guanylate Cyclase</subject><subject>Male</subject><subject>Marine</subject><subject>Molecular Structure</subject><subject>Peptides</subject><subject>Photoreception</subject><subject>Physiological aspects</subject><subject>Protein Binding</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Sea Urchins - physiology</subject><subject>Signal Transduction - physiology</subject><subject>Sperm</subject><subject>Sperm Motility</subject><subject>Spermatozoa</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - metabolism</subject><subject>Spermatozoa - physiology</subject><subject>Swimming</subject><issn>1465-7392</issn><issn>1476-4679</issn><issn>1476-4679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0t2L1DAQAPAgiveh_gUiQUHwoWeSNl-Px-HpwYKg-x7SdLKbo03WpOXO_96WLcqKIHlISH4zMJNB6BUlV5TU6mN0rab8CTqnjRRVI6R-upwFr2St2Rm6KOWeENo0RD5HZ5RxUTMpztFmuwdcwi7aHvs-PWAbOzykMWWcoRxSLIBdimNOfYg77PYwP9rHUHDyuIDFU3b7EHE5QB5eoGfe9gVervsl2t5-2t58qTZfP9_dXG8qxxkdK95o4Z3zRDEm67Ztveoc6MYB875j4BsuNCGEO0Y7xQlvtXO2doJ6KwjUl-j9Me0hpx8TlNEMoTjoexshTcVIprVgqvkvpEpxxvUC3_4F79OU554UwxiholGMzOjdEe1sDyZEn8Zs3ZLRXFNVMyWl1rO6-oeaVwdDmDsJPsz3JwEfTgKWbsPjuLNTKebu-7dTuxbkciolgzeHHAabfxpKzDIH5jgHM3yzFjS1A3R_2PrxM3h9BNGOU4bfYI3_BUUvtPM</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>Kaupp, U. Benjamin</creator><creator>Solzin, Johannes</creator><creator>Hildebrand, Eilo</creator><creator>Brown, Joel E</creator><creator>Helbig, Annika</creator><creator>Hagen, Volker</creator><creator>Beyermann, Michael</creator><creator>Pampaloni, Francesco</creator><creator>Weyand, Ingo</creator><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20030201</creationdate><title>The signal flow and motor response controling chemotaxis of sea urchin sperm</title><author>Kaupp, U. Benjamin ; Solzin, Johannes ; Hildebrand, Eilo ; Brown, Joel E ; Helbig, Annika ; Hagen, Volker ; Beyermann, Michael ; Pampaloni, Francesco ; Weyand, Ingo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-5496fccf082273bbbf8dce94ce2ffd2ef45690005c21d8505b9cca3c61fa60e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Arbacia punctulata</topic><topic>Calcium - metabolism</topic><topic>Calcium ions</topic><topic>Chemotaxis</topic><topic>Chemotaxis - physiology</topic><topic>Cyclic AMP - analogs & derivatives</topic><topic>Cyclic AMP - metabolism</topic><topic>Cyclic GMP - analogs & derivatives</topic><topic>Cyclic GMP - metabolism</topic><topic>Egg Proteins - metabolism</topic><topic>Guanylate Cyclase</topic><topic>Male</topic><topic>Marine</topic><topic>Molecular Structure</topic><topic>Peptides</topic><topic>Photoreception</topic><topic>Physiological aspects</topic><topic>Protein Binding</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Sea Urchins - physiology</topic><topic>Signal Transduction - physiology</topic><topic>Sperm</topic><topic>Sperm Motility</topic><topic>Spermatozoa</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - metabolism</topic><topic>Spermatozoa - physiology</topic><topic>Swimming</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaupp, U. Benjamin</creatorcontrib><creatorcontrib>Solzin, Johannes</creatorcontrib><creatorcontrib>Hildebrand, Eilo</creatorcontrib><creatorcontrib>Brown, Joel E</creatorcontrib><creatorcontrib>Helbig, Annika</creatorcontrib><creatorcontrib>Hagen, Volker</creatorcontrib><creatorcontrib>Beyermann, Michael</creatorcontrib><creatorcontrib>Pampaloni, Francesco</creatorcontrib><creatorcontrib>Weyand, Ingo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Nature cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaupp, U. Benjamin</au><au>Solzin, Johannes</au><au>Hildebrand, Eilo</au><au>Brown, Joel E</au><au>Helbig, Annika</au><au>Hagen, Volker</au><au>Beyermann, Michael</au><au>Pampaloni, Francesco</au><au>Weyand, Ingo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The signal flow and motor response controling chemotaxis of sea urchin sperm</atitle><jtitle>Nature cell biology</jtitle><addtitle>Nat Cell Biol</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>5</volume><issue>2</issue><spage>109</spage><epage>117</epage><pages>109-117</pages><issn>1465-7392</issn><issn>1476-4679</issn><eissn>1476-4679</eissn><abstract>The signalling pathway and the behavioural strategy underlying chemotaxis of sperm are poorly understood. We have studied the cellular events and motor responses that mediate chemotaxis of sperm from the sea urchin Arbacia punctulata. Here we show that resact, a chemoattractant peptide, initiates a rapid and transient rise in the concentration of cyclic GMP, followed by a transient influx of Ca2+. The binding of a single resact molecule elicits a Ca2+ response, and 50-100 bound molecules saturate the response. The ability to register single molecules is reminiscent of the single-photon sensitivity of rod photoreceptors. Both resact and cyclic nucleotides cause a turn or brief tumbling in the swimming path of sperm. We conclude that a cGMP-mediated increase in the Ca2+ concentration induces the primary motor response of sperm to the chemoattractant.</abstract><cop>England</cop><pub>Nature Publishing Group</pub><pmid>12563276</pmid><doi>10.1038/ncb915</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1465-7392 |
| ispartof | Nature cell biology, 2003-02, Vol.5 (2), p.109-117 |
| issn | 1465-7392 1476-4679 1476-4679 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72996284 |
| source | MEDLINE; Nature Journals Online; Alma/SFX Local Collection |
| subjects | Animals Arbacia punctulata Calcium - metabolism Calcium ions Chemotaxis Chemotaxis - physiology Cyclic AMP - analogs & derivatives Cyclic AMP - metabolism Cyclic GMP - analogs & derivatives Cyclic GMP - metabolism Egg Proteins - metabolism Guanylate Cyclase Male Marine Molecular Structure Peptides Photoreception Physiological aspects Protein Binding Receptors, Cell Surface - metabolism Sea Urchins - physiology Signal Transduction - physiology Sperm Sperm Motility Spermatozoa Spermatozoa - cytology Spermatozoa - metabolism Spermatozoa - physiology Swimming |
| title | The signal flow and motor response controling chemotaxis of sea urchin sperm |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-11-30T05%3A56%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20signal%20flow%20and%20motor%20response%20controling%20chemotaxis%20of%20sea%20urchin%20sperm&rft.jtitle=Nature%20cell%20biology&rft.au=Kaupp,%20U.%20Benjamin&rft.date=2003-02-01&rft.volume=5&rft.issue=2&rft.spage=109&rft.epage=117&rft.pages=109-117&rft.issn=1465-7392&rft.eissn=1476-4679&rft_id=info:doi/10.1038/ncb915&rft_dat=%3Cgale_proqu%3EA183287799%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=220164820&rft_id=info:pmid/12563276&rft_galeid=A183287799&rfr_iscdi=true |