Determination of glucose in whole blood samples by mid-infrared spectroscopy

Determination of glucose in whole blood samples by mid-infrared spectroscopy

We have determined the glucose concentration of whole blood from mid-infrared spectra without sample preparation or use of chemical reagents. We selected 1119-1022 cm(-1) as the optimal wavelength range for our measurement by making a first-loading vector analysis based on partial least-squares regr...

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Veröffentlicht in:Applied Optics 2003-02, Vol.42 (4), p.745-749
Hauptverfasser: Kim, Yoen-Joo, Hahn, Sangjoon, Yoon, Gilwon
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Sprache:eng
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Blood Glucose - analysis
Hemoglobins - analysis
Humans
Spectrophotometry, Infrared
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container_title Applied Optics
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creator Kim, Yoen-Joo
Hahn, Sangjoon
Yoon, Gilwon
description We have determined the glucose concentration of whole blood from mid-infrared spectra without sample preparation or use of chemical reagents. We selected 1119-1022 cm(-1) as the optimal wavelength range for our measurement by making a first-loading vector analysis based on partial least-squares regression. We examined the influence of hemoglobin on samples by using different calibration and prediction sets. The accuracy of glucose prediction depended on the hemoglobin level in the calibration model; the sample set should represent the entire range of hemoglobin concentration. We obtained an accuracy of 5.9% in glucose prediction, and this value is well within a clinically acceptable range.
doi_str_mv 10.1364/ao.42.000745
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source MEDLINE; Alma/SFX Local Collection; Optica Publishing Group Journals
subjects Blood Glucose - analysis
Hemoglobins - analysis
Humans
Spectrophotometry, Infrared
title Determination of glucose in whole blood samples by mid-infrared spectroscopy
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