Binding and functional properties of the extrinsic proteins in oxygen-evolving photosystem II particle from a green alga, Chlamydomonas reinhardtii having his-tagged CP47

Oxygen-evolving photosystem II (PSII) particles were purified from Chlamydomonas reinhardtii having His-tag extension at the C terminus of the CP47 protein, by a single-step Ni2+-affinity column chromatography after solubilization of thylakoid membranes with sucrose monolaurate. The PSII particles c...

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Veröffentlicht in:	Plant and cell physiology 2003-01, Vol.44 (1), p.76-84
Hauptverfasser:	Suzuki, T. (Science Univ. of Tokyo, Noda, Chiba (Japan). Faculty of Science and Technology), Minagawa, J, Tomo, T, Sonoike, K, Ohta, H, Enami, I
Format:	Artikel
Sprache:	eng
Schlagworte:	5-dimethyl-p-benzoquinone 6-dichloro-p-benzoquinone Algal Proteins - metabolism Animals BINDING PROTEINS Binding Sites Calcium - pharmacology Calcium Chloride - pharmacology CELL STRUCTURE CHLAMYDOMONAS REINHARDTII Chlamydomonas reinhardtii - drug effects Chlamydomonas reinhardtii - metabolism Chlorides - pharmacology Chlorophyta Chromatography, Affinity CP47 protein DCBQ Diuron - pharmacology DMBQ Electron Transport - drug effects Ferricyanides - metabolism Freshwater Keywords: Chlamydomonas reinhardtii — CP47 — Extrinsic protein — His-tag — Oxygen evolution — PSII complex Magnesium - pharmacology Oxygen - metabolism PBQ phenyl-p-benzoquinone Photosynthesis - physiology PHOTOSYSTEMS Protein Binding - drug effects Sodium Chloride - pharmacology Thylakoids - drug effects Thylakoids - metabolism
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