p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry
Mutation and overexpression of the p53 gene have been noted in a wide range of human cancers and are thought to play a role in malignant transformation. Previously, immunohistochemical detection of p53 has been possible only in fresh-frozen tissues. We examined p53 expression in paraffin-embedded ti...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1992-07, Vol.40 (7), p.1047-1051 |
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| container_issue | 7 |
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| container_title | The journal of histochemistry and cytochemistry |
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| creator | Kerns, BJ Jordan, PA Moore, MB Humphrey, PA Berchuck, A Kohler, MF Bast, RC, Jr Iglehart, JD Marks, JR |
| description | Mutation and overexpression of the p53 gene have been noted in a wide range of human cancers and are thought to play a role in malignant transformation. Previously, immunohistochemical detection of p53 has been possible only in fresh-frozen tissues. We examined p53 expression in paraffin-embedded tissues from 50 epithelial ovarian cancers and 25 primary breast cancers with a modified immunohistochemical (IHC) technique developed in this laboratory, using monoclonal antibody (MAb) PAb1801. The 75 cases were selected from a group of patients in whom the expression levels had already been assessed in a fresh-tissue IHC assay. An identical staining reactivity was observed in both formalin-fixed, paraffin-embedded tissue and fresh-frozen tissue in 48 of 50 (96%) epithelial ovarian cancers and in 23 of 25 (92%) primary breast cancers. Immunodetection of p53 in paraffin-embedded tissue blocks will be a useful alternative to the standard fresh-tissue assay and can accurately reflect the level of p53 expression in human tumors. |
| doi_str_mv | 10.1177/40.7.1607637 |
| format | Article |
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Immunodetection of p53 in paraffin-embedded tissue blocks will be a useful alternative to the standard fresh-tissue assay and can accurately reflect the level of p53 expression in human tumors.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1177/40.7.1607637</identifier><identifier>PMID: 1607637</identifier><identifier>CODEN: JHCYAS</identifier><language>eng</language><publisher>Los Angeles, CA: Histochemical Soc</publisher><subject>Analytical, structural and metabolic biochemistry ; Antibodies, Monoclonal ; Biological and medical sciences ; Breast Neoplasms - chemistry ; Carcinoma - chemistry ; Female ; Fundamental and applied biological sciences. 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Previously, immunohistochemical detection of p53 has been possible only in fresh-frozen tissues. We examined p53 expression in paraffin-embedded tissues from 50 epithelial ovarian cancers and 25 primary breast cancers with a modified immunohistochemical (IHC) technique developed in this laboratory, using monoclonal antibody (MAb) PAb1801. The 75 cases were selected from a group of patients in whom the expression levels had already been assessed in a fresh-tissue IHC assay. An identical staining reactivity was observed in both formalin-fixed, paraffin-embedded tissue and fresh-frozen tissue in 48 of 50 (96%) epithelial ovarian cancers and in 23 of 25 (92%) primary breast cancers. Immunodetection of p53 in paraffin-embedded tissue blocks will be a useful alternative to the standard fresh-tissue assay and can accurately reflect the level of p53 expression in human tumors.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Breast Neoplasms - chemistry</subject><subject>Carcinoma - chemistry</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Ovarian Neoplasms - chemistry</subject><subject>Proteins</subject><subject>Tissue Embedding</subject><subject>Tissue Fixation</subject><subject>Tumor Suppressor Protein p53 - analysis</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1LxDAQhoMouq7evAo9iCDYNUnTJj2K-AWCFz2HbDJxI21Tk9Z1_72RLXryNMzMw8w77yB0QvCCEM6vGF7wBakwrwq-g2akLEleYsZ20QxjSvNUYAfoMMZ3jAljpdhH-xM-Q7Ivi8x_QoCvPkCMzneZ6zLrQ6sa1-XWfYG5zHoVlLUph3YJxoDJBhfjCJmBAfSQ8uUmc207dn7l4uD1CtoUw-YI7VnVRDie4hy93t2-3DzkT8_3jzfXT7lmRAw5EawiQqmKUiEsE0ZQAcSYSlFcWG45VoQqKixVJFGlxVqXVV1oDlrUmhZzdL6d2wf_MUIcZNqvoWlUB36MktO6LmohEni5BXXwMQawsg-uVWEjCZY_fkqGJZeTQQk_neaOyxbMH_zbP5v6KmrV2KA67eIvxhJCk845uthiUb2BfPdj6JIb_62cTlm5t9XaBZAx_aJJAohcr9dbFjNefAMO-ZdT</recordid><startdate>19920701</startdate><enddate>19920701</enddate><creator>Kerns, BJ</creator><creator>Jordan, PA</creator><creator>Moore, MB</creator><creator>Humphrey, PA</creator><creator>Berchuck, A</creator><creator>Kohler, MF</creator><creator>Bast, RC, Jr</creator><creator>Iglehart, JD</creator><creator>Marks, JR</creator><general>Histochemical Soc</general><general>SAGE Publications</general><general>Histochemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920701</creationdate><title>p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry</title><author>Kerns, BJ ; Jordan, PA ; Moore, MB ; Humphrey, PA ; Berchuck, A ; Kohler, MF ; Bast, RC, Jr ; Iglehart, JD ; Marks, JR</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-184618aa62288f48d828e1dd6a203f7f70a12a28f2a1aa65f0cc5693c7ec89c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Breast Neoplasms - chemistry</topic><topic>Carcinoma - chemistry</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Ovarian Neoplasms - chemistry</topic><topic>Proteins</topic><topic>Tissue Embedding</topic><topic>Tissue Fixation</topic><topic>Tumor Suppressor Protein p53 - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kerns, BJ</creatorcontrib><creatorcontrib>Jordan, PA</creatorcontrib><creatorcontrib>Moore, MB</creatorcontrib><creatorcontrib>Humphrey, PA</creatorcontrib><creatorcontrib>Berchuck, A</creatorcontrib><creatorcontrib>Kohler, MF</creatorcontrib><creatorcontrib>Bast, RC, Jr</creatorcontrib><creatorcontrib>Iglehart, JD</creatorcontrib><creatorcontrib>Marks, JR</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kerns, BJ</au><au>Jordan, PA</au><au>Moore, MB</au><au>Humphrey, PA</au><au>Berchuck, A</au><au>Kohler, MF</au><au>Bast, RC, Jr</au><au>Iglehart, JD</au><au>Marks, JR</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>1992-07-01</date><risdate>1992</risdate><volume>40</volume><issue>7</issue><spage>1047</spage><epage>1051</epage><pages>1047-1051</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><coden>JHCYAS</coden><abstract>Mutation and overexpression of the p53 gene have been noted in a wide range of human cancers and are thought to play a role in malignant transformation. Previously, immunohistochemical detection of p53 has been possible only in fresh-frozen tissues. We examined p53 expression in paraffin-embedded tissues from 50 epithelial ovarian cancers and 25 primary breast cancers with a modified immunohistochemical (IHC) technique developed in this laboratory, using monoclonal antibody (MAb) PAb1801. The 75 cases were selected from a group of patients in whom the expression levels had already been assessed in a fresh-tissue IHC assay. An identical staining reactivity was observed in both formalin-fixed, paraffin-embedded tissue and fresh-frozen tissue in 48 of 50 (96%) epithelial ovarian cancers and in 23 of 25 (92%) primary breast cancers. Immunodetection of p53 in paraffin-embedded tissue blocks will be a useful alternative to the standard fresh-tissue assay and can accurately reflect the level of p53 expression in human tumors.</abstract><cop>Los Angeles, CA</cop><pub>Histochemical Soc</pub><pmid>1607637</pmid><doi>10.1177/40.7.1607637</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Antibodies, Monoclonal Biological and medical sciences Breast Neoplasms - chemistry Carcinoma - chemistry Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Male Ovarian Neoplasms - chemistry Proteins Tissue Embedding Tissue Fixation Tumor Suppressor Protein p53 - analysis |
| title | p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry |
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