Effect of dexamethasone on nucleolar casein kinase II activity and phosphorylation of nucleolin in lymphosarcoma P1798 cells
Ribosomal RNA (rRNA) synthesis in murine P1798 lymphosarcoma cells is reversibly inhibited by glucocorticoids. The effects of dexamethasone upon nucleolin phosphorylation and upon the amount and activity of casein kinase II have been examined. P1798 cells were exposed to 0.1 μM dexamethasone for 36...
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| Veröffentlicht in: | The Journal of steroid biochemistry and molecular biology 1992-05, Vol.42 (3), p.305-312 |
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| creator | Suzuki, Noriaki Suzuki, Toshikazu Uchida, Aoi Thompson, E.Aubrey Hosoya, Toichiro |
| description | Ribosomal RNA (rRNA) synthesis in murine
P1798 lymphosarcoma cells is reversibly inhibited by glucocorticoids. The effects of dexamethasone upon nucleolin phosphorylation and upon the amount and activity of casein kinase II have been examined.
P1798 cells were exposed to 0.1 μM dexamethasone for 36 h. Cells were labeled
in vivo with [
32P]orthophosphate followed by immunoprecipitation with anti-nucleolin antibody. Nucleolin phosphorylation was reduced by 60% in dexamethasone-treated cells. Nucleoli were isolated and labeled with [γ-
32P]ATP
in vitro. Nucleolin protein was reduced to 40% of control in nuclei from dexamethasone-treated cells. Nucleolin phosphorylation was reduced to 20% of control. Nucleolar casein kinase II activity and protein were also reduced (30–55% and 35–50% of control, respectively) by treatment with dexamethasone. Cycloheximide (10 μg/ml for 3 h) reduced the amount and activity of casein kinase II, but did not cause a decrease in nucleolin protein. These observations are discussed relative to the hypothesis that glucocorticoids regulate the amount or activity of proteins of short biological half-life that are involved in the regulation of rRNA synthesis. |
| doi_str_mv | 10.1016/0960-0760(92)90133-4 |
| format | Article |
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P1798 lymphosarcoma cells is reversibly inhibited by glucocorticoids. The effects of dexamethasone upon nucleolin phosphorylation and upon the amount and activity of casein kinase II have been examined.
P1798 cells were exposed to 0.1 μM dexamethasone for 36 h. Cells were labeled
in vivo with [
32P]orthophosphate followed by immunoprecipitation with anti-nucleolin antibody. Nucleolin phosphorylation was reduced by 60% in dexamethasone-treated cells. Nucleoli were isolated and labeled with [γ-
32P]ATP
in vitro. Nucleolin protein was reduced to 40% of control in nuclei from dexamethasone-treated cells. Nucleolin phosphorylation was reduced to 20% of control. Nucleolar casein kinase II activity and protein were also reduced (30–55% and 35–50% of control, respectively) by treatment with dexamethasone. Cycloheximide (10 μg/ml for 3 h) reduced the amount and activity of casein kinase II, but did not cause a decrease in nucleolin protein. These observations are discussed relative to the hypothesis that glucocorticoids regulate the amount or activity of proteins of short biological half-life that are involved in the regulation of rRNA synthesis.</description><identifier>ISSN: 0960-0760</identifier><identifier>EISSN: 1879-1220</identifier><identifier>DOI: 10.1016/0960-0760(92)90133-4</identifier><identifier>PMID: 1606042</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Casein Kinase II ; Cell Nucleolus - enzymology ; Cells, Cultured ; Cycloheximide - pharmacology ; Dexamethasone - pharmacology ; DNA - biosynthesis ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Mice ; Nuclear Proteins - metabolism ; Nucleolin ; Phosphoproteins - metabolism ; Phosphorylation ; Protein Serine-Threonine Kinases - metabolism ; RNA, Ribosomal - biosynthesis ; RNA-Binding Proteins ; Transferases</subject><ispartof>The Journal of steroid biochemistry and molecular biology, 1992-05, Vol.42 (3), p.305-312</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-8cad21e13853389eedffe267aafbff1f74a0a3c6449119d069f4d7153e3ef9913</citedby><cites>FETCH-LOGICAL-c386t-8cad21e13853389eedffe267aafbff1f74a0a3c6449119d069f4d7153e3ef9913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0960-0760(92)90133-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5353923$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1606042$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki, Noriaki</creatorcontrib><creatorcontrib>Suzuki, Toshikazu</creatorcontrib><creatorcontrib>Uchida, Aoi</creatorcontrib><creatorcontrib>Thompson, E.Aubrey</creatorcontrib><creatorcontrib>Hosoya, Toichiro</creatorcontrib><title>Effect of dexamethasone on nucleolar casein kinase II activity and phosphorylation of nucleolin in lymphosarcoma P1798 cells</title><title>The Journal of steroid biochemistry and molecular biology</title><addtitle>J Steroid Biochem Mol Biol</addtitle><description>Ribosomal RNA (rRNA) synthesis in murine
P1798 lymphosarcoma cells is reversibly inhibited by glucocorticoids. The effects of dexamethasone upon nucleolin phosphorylation and upon the amount and activity of casein kinase II have been examined.
P1798 cells were exposed to 0.1 μM dexamethasone for 36 h. Cells were labeled
in vivo with [
32P]orthophosphate followed by immunoprecipitation with anti-nucleolin antibody. Nucleolin phosphorylation was reduced by 60% in dexamethasone-treated cells. Nucleoli were isolated and labeled with [γ-
32P]ATP
in vitro. Nucleolin protein was reduced to 40% of control in nuclei from dexamethasone-treated cells. Nucleolin phosphorylation was reduced to 20% of control. Nucleolar casein kinase II activity and protein were also reduced (30–55% and 35–50% of control, respectively) by treatment with dexamethasone. Cycloheximide (10 μg/ml for 3 h) reduced the amount and activity of casein kinase II, but did not cause a decrease in nucleolin protein. These observations are discussed relative to the hypothesis that glucocorticoids regulate the amount or activity of proteins of short biological half-life that are involved in the regulation of rRNA synthesis.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Casein Kinase II</subject><subject>Cell Nucleolus - enzymology</subject><subject>Cells, Cultured</subject><subject>Cycloheximide - pharmacology</subject><subject>Dexamethasone - pharmacology</subject><subject>DNA - biosynthesis</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Mice</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nucleolin</subject><subject>Phosphoproteins - metabolism</subject><subject>Phosphorylation</subject><subject>Protein Serine-Threonine Kinases - metabolism</subject><subject>RNA, Ribosomal - biosynthesis</subject><subject>RNA-Binding Proteins</subject><subject>Transferases</subject><issn>0960-0760</issn><issn>1879-1220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV9LHDEUxUOp2K3tN7CQh1Lqw9j8mc1MXoQitl0Q9ME-h2tyg7EzyTaZFRf88Ga6i30TEnLh_s7hcELIMWennHH1jWnFGtYp9lWLE824lE37hix43-mGC8HeksUL8o68L-WeMSYl7w7JIVdMsVYsyNOF92gnmjx1-AgjTndQUkSaIo0bO2AaIFMLBUOkf0KsA12tKNgpPIRpSyE6ur5Lpd68HWAKVVe99tKqqWfYjjMC2aYR6DXvdE8tDkP5QA48DAU_7t8j8vvHxc35r-by6ufq_PtlY2Wvpqa34ARHLvullL1GdDWzUB2Av_We-64FBtKqttWca8eU9q3r-FKiRK81l0fky853ndPfDZbJjKHMCSBi2hTTCa2F0KyC7Q60OZWS0Zt1DiPkreHMzKWbuVEzN2q0MP9KN22Vfdr7b25HdP9Fu5br_vN-D8XC4DNEG8oLtpRLqYWs2NkOw9rFQ8Bsig0YLbqQ6x8Zl8LrOZ4Bm4We_A</recordid><startdate>19920501</startdate><enddate>19920501</enddate><creator>Suzuki, Noriaki</creator><creator>Suzuki, Toshikazu</creator><creator>Uchida, Aoi</creator><creator>Thompson, E.Aubrey</creator><creator>Hosoya, Toichiro</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920501</creationdate><title>Effect of dexamethasone on nucleolar casein kinase II activity and phosphorylation of nucleolin in lymphosarcoma P1798 cells</title><author>Suzuki, Noriaki ; Suzuki, Toshikazu ; Uchida, Aoi ; Thompson, E.Aubrey ; Hosoya, Toichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-8cad21e13853389eedffe267aafbff1f74a0a3c6449119d069f4d7153e3ef9913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Casein Kinase II</topic><topic>Cell Nucleolus - enzymology</topic><topic>Cells, Cultured</topic><topic>Cycloheximide - pharmacology</topic><topic>Dexamethasone - pharmacology</topic><topic>DNA - biosynthesis</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Mice</topic><topic>Nuclear Proteins - metabolism</topic><topic>Nucleolin</topic><topic>Phosphoproteins - metabolism</topic><topic>Phosphorylation</topic><topic>Protein Serine-Threonine Kinases - metabolism</topic><topic>RNA, Ribosomal - biosynthesis</topic><topic>RNA-Binding Proteins</topic><topic>Transferases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki, Noriaki</creatorcontrib><creatorcontrib>Suzuki, Toshikazu</creatorcontrib><creatorcontrib>Uchida, Aoi</creatorcontrib><creatorcontrib>Thompson, E.Aubrey</creatorcontrib><creatorcontrib>Hosoya, Toichiro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Noriaki</au><au>Suzuki, Toshikazu</au><au>Uchida, Aoi</au><au>Thompson, E.Aubrey</au><au>Hosoya, Toichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of dexamethasone on nucleolar casein kinase II activity and phosphorylation of nucleolin in lymphosarcoma P1798 cells</atitle><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle><addtitle>J Steroid Biochem Mol Biol</addtitle><date>1992-05-01</date><risdate>1992</risdate><volume>42</volume><issue>3</issue><spage>305</spage><epage>312</epage><pages>305-312</pages><issn>0960-0760</issn><eissn>1879-1220</eissn><abstract>Ribosomal RNA (rRNA) synthesis in murine
P1798 lymphosarcoma cells is reversibly inhibited by glucocorticoids. The effects of dexamethasone upon nucleolin phosphorylation and upon the amount and activity of casein kinase II have been examined.
P1798 cells were exposed to 0.1 μM dexamethasone for 36 h. Cells were labeled
in vivo with [
32P]orthophosphate followed by immunoprecipitation with anti-nucleolin antibody. Nucleolin phosphorylation was reduced by 60% in dexamethasone-treated cells. Nucleoli were isolated and labeled with [γ-
32P]ATP
in vitro. Nucleolin protein was reduced to 40% of control in nuclei from dexamethasone-treated cells. Nucleolin phosphorylation was reduced to 20% of control. Nucleolar casein kinase II activity and protein were also reduced (30–55% and 35–50% of control, respectively) by treatment with dexamethasone. Cycloheximide (10 μg/ml for 3 h) reduced the amount and activity of casein kinase II, but did not cause a decrease in nucleolin protein. These observations are discussed relative to the hypothesis that glucocorticoids regulate the amount or activity of proteins of short biological half-life that are involved in the regulation of rRNA synthesis.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1606042</pmid><doi>10.1016/0960-0760(92)90133-4</doi><tpages>8</tpages></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Casein Kinase II Cell Nucleolus - enzymology Cells, Cultured Cycloheximide - pharmacology Dexamethasone - pharmacology DNA - biosynthesis Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology In Vitro Techniques Mice Nuclear Proteins - metabolism Nucleolin Phosphoproteins - metabolism Phosphorylation Protein Serine-Threonine Kinases - metabolism RNA, Ribosomal - biosynthesis RNA-Binding Proteins Transferases |
| title | Effect of dexamethasone on nucleolar casein kinase II activity and phosphorylation of nucleolin in lymphosarcoma P1798 cells |
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