A molecular epidemiological study of Australian bat lyssavirus
1 Department of Microbiology and Parasitology, University of Queensland, St Lucia, Queensland, Australia 2 Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Level 4, Gehrmann Laboratories, University of Queensland, St Lucia, Queensland, Australia 3 Department of Zoology...
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| Veröffentlicht in: | Journal of general virology 2003-02, Vol.84 (2), p.485-496 |
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| creator | Guyatt, Kimberley J Twin, Jimmy Davis, Patricia Holmes, Edward C Smith, Greg A Smith, Ina L Mackenzie, John S Young, Peter L |
| description | 1 Department of Microbiology and Parasitology, University of Queensland, St Lucia, Queensland, Australia
2 Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Level 4, Gehrmann Laboratories, University of Queensland, St Lucia, Queensland, Australia
3 Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
4 Unité de la Rage, Institut Pasteur, Paris Cedex 15, France
5 Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia
Correspondence Kimberley Guyatt (at Queensland Agricultural Biotechnology Centre) k.guyatt{at}mailbox.uq.edu.au
The genetic diversity of Australian bat lyssavirus (ABL) was investigated by comparing 24 ABL isolate glycoprotein (G) gene nucleotide sequences with those of 37 lyssaviruses representing Lyssavirus genotypes 16. Phylogenetic analyses indicated that ABL forms a monophyletic group separate from other lyssaviruses. This group differentiates into two clades: one associated with Pteropus (flying fox) species, the other with the insectivorous bat Saccolaimus flaviventris . Calculation of percentage nucleotide identities between isolates of the two clades revealed up to 18·7 % nucleotide sequence divergence between the two ABL variants. These observations suggest that ABL is a separate lyssavirus species with a similar epidemiology to chiropteran rabies virus (RV), where two distinct ABL variants co-exist in Australia in bat species with dissimilar ecology. Analyses of selection pressures in ABL G gene sequences provided some evidence of weak positive selection within the endodomain at amino acids 499 and 501, although in general the dominant evolutionary process observed was purifying selection. This intimates that, in nature, isolates of ABL, like those of RV, are subject to relatively strong selective constraints, suggesting a stability of host species, cell tropisms and ecological conditions.
The GenBank accession numbers of the sequences reported in this paper are AF426290 AF426311 . |
| doi_str_mv | 10.1099/vir.0.18652-0 |
| format | Article |
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2 Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Level 4, Gehrmann Laboratories, University of Queensland, St Lucia, Queensland, Australia
3 Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
4 Unité de la Rage, Institut Pasteur, Paris Cedex 15, France
5 Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia
Correspondence Kimberley Guyatt (at Queensland Agricultural Biotechnology Centre) k.guyatt{at}mailbox.uq.edu.au
The genetic diversity of Australian bat lyssavirus (ABL) was investigated by comparing 24 ABL isolate glycoprotein (G) gene nucleotide sequences with those of 37 lyssaviruses representing Lyssavirus genotypes 16. Phylogenetic analyses indicated that ABL forms a monophyletic group separate from other lyssaviruses. This group differentiates into two clades: one associated with Pteropus (flying fox) species, the other with the insectivorous bat Saccolaimus flaviventris . Calculation of percentage nucleotide identities between isolates of the two clades revealed up to 18·7 % nucleotide sequence divergence between the two ABL variants. These observations suggest that ABL is a separate lyssavirus species with a similar epidemiology to chiropteran rabies virus (RV), where two distinct ABL variants co-exist in Australia in bat species with dissimilar ecology. Analyses of selection pressures in ABL G gene sequences provided some evidence of weak positive selection within the endodomain at amino acids 499 and 501, although in general the dominant evolutionary process observed was purifying selection. This intimates that, in nature, isolates of ABL, like those of RV, are subject to relatively strong selective constraints, suggesting a stability of host species, cell tropisms and ecological conditions.
The GenBank accession numbers of the sequences reported in this paper are AF426290 AF426311 .</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/vir.0.18652-0</identifier><identifier>PMID: 12560583</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Animals ; Australia ; Australian bat lyssavirus ; Brain ; Cats ; Chiroptera - virology ; Dogs ; Genetic Variation ; Humans ; Lyssavirus - classification ; Lyssavirus - genetics ; Molecular Epidemiology ; Molecular Sequence Data ; Phylogeny ; Pteropus ; Rhabdoviridae Infections - veterinary ; Rhabdoviridae Infections - virology ; Saccolaimus flaviventris ; Sequence Analysis, DNA ; Viral Proteins - genetics</subject><ispartof>Journal of general virology, 2003-02, Vol.84 (2), p.485-496</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c323t-1960ac60dfd216d48fd0c5a73c9a56c4aa114060ddf70de924b4636ed08ee87d3</citedby><cites>FETCH-LOGICAL-c323t-1960ac60dfd216d48fd0c5a73c9a56c4aa114060ddf70de924b4636ed08ee87d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12560583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guyatt, Kimberley J</creatorcontrib><creatorcontrib>Twin, Jimmy</creatorcontrib><creatorcontrib>Davis, Patricia</creatorcontrib><creatorcontrib>Holmes, Edward C</creatorcontrib><creatorcontrib>Smith, Greg A</creatorcontrib><creatorcontrib>Smith, Ina L</creatorcontrib><creatorcontrib>Mackenzie, John S</creatorcontrib><creatorcontrib>Young, Peter L</creatorcontrib><title>A molecular epidemiological study of Australian bat lyssavirus</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 Department of Microbiology and Parasitology, University of Queensland, St Lucia, Queensland, Australia
2 Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Level 4, Gehrmann Laboratories, University of Queensland, St Lucia, Queensland, Australia
3 Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
4 Unité de la Rage, Institut Pasteur, Paris Cedex 15, France
5 Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia
Correspondence Kimberley Guyatt (at Queensland Agricultural Biotechnology Centre) k.guyatt{at}mailbox.uq.edu.au
The genetic diversity of Australian bat lyssavirus (ABL) was investigated by comparing 24 ABL isolate glycoprotein (G) gene nucleotide sequences with those of 37 lyssaviruses representing Lyssavirus genotypes 16. Phylogenetic analyses indicated that ABL forms a monophyletic group separate from other lyssaviruses. This group differentiates into two clades: one associated with Pteropus (flying fox) species, the other with the insectivorous bat Saccolaimus flaviventris . Calculation of percentage nucleotide identities between isolates of the two clades revealed up to 18·7 % nucleotide sequence divergence between the two ABL variants. These observations suggest that ABL is a separate lyssavirus species with a similar epidemiology to chiropteran rabies virus (RV), where two distinct ABL variants co-exist in Australia in bat species with dissimilar ecology. Analyses of selection pressures in ABL G gene sequences provided some evidence of weak positive selection within the endodomain at amino acids 499 and 501, although in general the dominant evolutionary process observed was purifying selection. This intimates that, in nature, isolates of ABL, like those of RV, are subject to relatively strong selective constraints, suggesting a stability of host species, cell tropisms and ecological conditions.
The GenBank accession numbers of the sequences reported in this paper are AF426290 AF426311 .</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Australia</subject><subject>Australian bat lyssavirus</subject><subject>Brain</subject><subject>Cats</subject><subject>Chiroptera - virology</subject><subject>Dogs</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Lyssavirus - classification</subject><subject>Lyssavirus - genetics</subject><subject>Molecular Epidemiology</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Pteropus</subject><subject>Rhabdoviridae Infections - veterinary</subject><subject>Rhabdoviridae Infections - virology</subject><subject>Saccolaimus flaviventris</subject><subject>Sequence Analysis, DNA</subject><subject>Viral Proteins - genetics</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotlaPXmVPeto6-djs5iKU4hcUvOg5ZJNsG8l2a7Kr9N-bpQWPnuaFeXiGeRG6xjDHIMT9twvzFCtekBxO0BQzXuQkbU7RFICQHFNcTtBFjJ8AmLGiPEcTTAoORUWn6GGRtZ23evAqZHbnjG1d57u108pnsR_MPuuabDHEPijv1DarVZ_5fYwqHR7iJTprlI_26jhn6OPp8X35kq_enl-Xi1WuKaF9jgUHpTmYxhDMDasaA7pQJdVCFVwzpTBmkPamKcFYQVjNOOXWQGVtVRo6Q7cH7y50X4ONvWxd1NZ7tbXdEGVJRCUE5f-CuBQMMIxgfgB16GIMtpG74FoV9hKDHJuV6UGZ4tishMTfHMVD3VrzRx-rTMDdAdi49ebHBSvXdtu6pK9dN8oqJolkVUF_AULrgnM</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>Guyatt, Kimberley J</creator><creator>Twin, Jimmy</creator><creator>Davis, Patricia</creator><creator>Holmes, Edward C</creator><creator>Smith, Greg A</creator><creator>Smith, Ina L</creator><creator>Mackenzie, John S</creator><creator>Young, Peter L</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030201</creationdate><title>A molecular epidemiological study of Australian bat lyssavirus</title><author>Guyatt, Kimberley J ; Twin, Jimmy ; Davis, Patricia ; Holmes, Edward C ; Smith, Greg A ; Smith, Ina L ; Mackenzie, John S ; Young, Peter L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-1960ac60dfd216d48fd0c5a73c9a56c4aa114060ddf70de924b4636ed08ee87d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Australia</topic><topic>Australian bat lyssavirus</topic><topic>Brain</topic><topic>Cats</topic><topic>Chiroptera - virology</topic><topic>Dogs</topic><topic>Genetic Variation</topic><topic>Humans</topic><topic>Lyssavirus - classification</topic><topic>Lyssavirus - genetics</topic><topic>Molecular Epidemiology</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Pteropus</topic><topic>Rhabdoviridae Infections - veterinary</topic><topic>Rhabdoviridae Infections - virology</topic><topic>Saccolaimus flaviventris</topic><topic>Sequence Analysis, DNA</topic><topic>Viral Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guyatt, Kimberley J</creatorcontrib><creatorcontrib>Twin, Jimmy</creatorcontrib><creatorcontrib>Davis, Patricia</creatorcontrib><creatorcontrib>Holmes, Edward C</creatorcontrib><creatorcontrib>Smith, Greg A</creatorcontrib><creatorcontrib>Smith, Ina L</creatorcontrib><creatorcontrib>Mackenzie, John S</creatorcontrib><creatorcontrib>Young, Peter L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guyatt, Kimberley J</au><au>Twin, Jimmy</au><au>Davis, Patricia</au><au>Holmes, Edward C</au><au>Smith, Greg A</au><au>Smith, Ina L</au><au>Mackenzie, John S</au><au>Young, Peter L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A molecular epidemiological study of Australian bat lyssavirus</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>84</volume><issue>2</issue><spage>485</spage><epage>496</epage><pages>485-496</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>1 Department of Microbiology and Parasitology, University of Queensland, St Lucia, Queensland, Australia
2 Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Level 4, Gehrmann Laboratories, University of Queensland, St Lucia, Queensland, Australia
3 Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
4 Unité de la Rage, Institut Pasteur, Paris Cedex 15, France
5 Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia
Correspondence Kimberley Guyatt (at Queensland Agricultural Biotechnology Centre) k.guyatt{at}mailbox.uq.edu.au
The genetic diversity of Australian bat lyssavirus (ABL) was investigated by comparing 24 ABL isolate glycoprotein (G) gene nucleotide sequences with those of 37 lyssaviruses representing Lyssavirus genotypes 16. Phylogenetic analyses indicated that ABL forms a monophyletic group separate from other lyssaviruses. This group differentiates into two clades: one associated with Pteropus (flying fox) species, the other with the insectivorous bat Saccolaimus flaviventris . Calculation of percentage nucleotide identities between isolates of the two clades revealed up to 18·7 % nucleotide sequence divergence between the two ABL variants. These observations suggest that ABL is a separate lyssavirus species with a similar epidemiology to chiropteran rabies virus (RV), where two distinct ABL variants co-exist in Australia in bat species with dissimilar ecology. Analyses of selection pressures in ABL G gene sequences provided some evidence of weak positive selection within the endodomain at amino acids 499 and 501, although in general the dominant evolutionary process observed was purifying selection. This intimates that, in nature, isolates of ABL, like those of RV, are subject to relatively strong selective constraints, suggesting a stability of host species, cell tropisms and ecological conditions.
The GenBank accession numbers of the sequences reported in this paper are AF426290 AF426311 .</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>12560583</pmid><doi>10.1099/vir.0.18652-0</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 2003-02, Vol.84 (2), p.485-496 |
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| subjects | Amino Acid Sequence Animals Australia Australian bat lyssavirus Brain Cats Chiroptera - virology Dogs Genetic Variation Humans Lyssavirus - classification Lyssavirus - genetics Molecular Epidemiology Molecular Sequence Data Phylogeny Pteropus Rhabdoviridae Infections - veterinary Rhabdoviridae Infections - virology Saccolaimus flaviventris Sequence Analysis, DNA Viral Proteins - genetics |
| title | A molecular epidemiological study of Australian bat lyssavirus |
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