A Miniature Biochip System for Detection of Aerosolized Bacillus globigii Spores
The feasibility of using a novel detection scheme for the analysis of biological warfare agents is demonstrated using Bacillus globigii spores, a surrogate species for Bacillus anthracis. In this paper, a sensitive and selective enzyme-linked immunosorbent assay using a novel fluorogenic alkaline ph...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2003-01, Vol.75 (2), p.275-280 |
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| creator | Stratis-Cullum, Dimitra N. Griffin, Guy D. Mobley, Joel Vass, Arpad A. Vo-Dinh, Tuan |
| description | The feasibility of using a novel detection scheme for the analysis of biological warfare agents is demonstrated using Bacillus globigii spores, a surrogate species for Bacillus anthracis. In this paper, a sensitive and selective enzyme-linked immunosorbent assay using a novel fluorogenic alkaline phosphatase substrate (dimethylacridinone phosphate) is combined with a compact biochip detection system, which includes a miniature diode laser for excitation. Detection of aerosolized spores was achieved by coupling the miniature system to a portable bioaerosol sampler, and the performance of the antibody-based recognition and enzyme amplification method was evaluated. The bioassay performance was found to be compatible with the air sampling device, and the enzymatic amplification was found to be an attractive amplification method for detection of low spore concentrations. The combined portable bioaerosol sampler and miniature biochip system detected 100 B. globigii spores, corresponding to 17 aerosolized spores/L of air. Moreover, the incorporation of the miniature diode laser with the self-contained biochip design allows for a compact system that is readily adaptable to field use. In addition, these studies have included investigations into the tradeoff between assay time and sensitivity. |
| doi_str_mv | 10.1021/ac026068+ |
| format | Article |
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In this paper, a sensitive and selective enzyme-linked immunosorbent assay using a novel fluorogenic alkaline phosphatase substrate (dimethylacridinone phosphate) is combined with a compact biochip detection system, which includes a miniature diode laser for excitation. Detection of aerosolized spores was achieved by coupling the miniature system to a portable bioaerosol sampler, and the performance of the antibody-based recognition and enzyme amplification method was evaluated. The bioassay performance was found to be compatible with the air sampling device, and the enzymatic amplification was found to be an attractive amplification method for detection of low spore concentrations. The combined portable bioaerosol sampler and miniature biochip system detected 100 B. globigii spores, corresponding to 17 aerosolized spores/L of air. Moreover, the incorporation of the miniature diode laser with the self-contained biochip design allows for a compact system that is readily adaptable to field use. In addition, these studies have included investigations into the tradeoff between assay time and sensitivity.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac026068+</identifier><identifier>PMID: 12553762</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Aerosols ; Anthrax - prevention & control ; Antigens, Bacterial - analysis ; Bacillus - immunology ; Bacillus anthracis ; Bacillus globigii ; Biological and medical sciences ; Biological Warfare ; biological warfare agents ; Biosensors ; Biotechnology ; Chemistry ; Enzyme-Linked Immunosorbent Assay - instrumentation ; Enzyme-Linked Immunosorbent Assay - standards ; Equipment Design ; Fundamental and applied biological sciences. Psychology ; Lasers ; Methods. Procedures. Technologies ; Miniaturization ; Spores, Bacterial - immunology ; Various methods and equipments</subject><ispartof>Analytical chemistry (Washington), 2003-01, Vol.75 (2), p.275-280</ispartof><rights>Copyright © 2003 American Chemical Society</rights><rights>2003 INIST-CNRS</rights><rights>Copyright American Chemical Society Jan 15, 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a537t-69a4ea4ee5151719024d164426e97c7201491fb96afc5c10c3b220e83693b4643</citedby><cites>FETCH-LOGICAL-a537t-69a4ea4ee5151719024d164426e97c7201491fb96afc5c10c3b220e83693b4643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac026068+$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac026068+$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14491489$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12553762$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stratis-Cullum, Dimitra N.</creatorcontrib><creatorcontrib>Griffin, Guy D.</creatorcontrib><creatorcontrib>Mobley, Joel</creatorcontrib><creatorcontrib>Vass, Arpad A.</creatorcontrib><creatorcontrib>Vo-Dinh, Tuan</creatorcontrib><title>A Miniature Biochip System for Detection of Aerosolized Bacillus globigii Spores</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>The feasibility of using a novel detection scheme for the analysis of biological warfare agents is demonstrated using Bacillus globigii spores, a surrogate species for Bacillus anthracis. In this paper, a sensitive and selective enzyme-linked immunosorbent assay using a novel fluorogenic alkaline phosphatase substrate (dimethylacridinone phosphate) is combined with a compact biochip detection system, which includes a miniature diode laser for excitation. Detection of aerosolized spores was achieved by coupling the miniature system to a portable bioaerosol sampler, and the performance of the antibody-based recognition and enzyme amplification method was evaluated. The bioassay performance was found to be compatible with the air sampling device, and the enzymatic amplification was found to be an attractive amplification method for detection of low spore concentrations. The combined portable bioaerosol sampler and miniature biochip system detected 100 B. globigii spores, corresponding to 17 aerosolized spores/L of air. Moreover, the incorporation of the miniature diode laser with the self-contained biochip design allows for a compact system that is readily adaptable to field use. In addition, these studies have included investigations into the tradeoff between assay time and sensitivity.</description><subject>Aerosols</subject><subject>Anthrax - prevention & control</subject><subject>Antigens, Bacterial - analysis</subject><subject>Bacillus - immunology</subject><subject>Bacillus anthracis</subject><subject>Bacillus globigii</subject><subject>Biological and medical sciences</subject><subject>Biological Warfare</subject><subject>biological warfare agents</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Enzyme-Linked Immunosorbent Assay - instrumentation</subject><subject>Enzyme-Linked Immunosorbent Assay - standards</subject><subject>Equipment Design</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lasers</subject><subject>Methods. Procedures. Technologies</subject><subject>Miniaturization</subject><subject>Spores, Bacterial - immunology</subject><subject>Various methods and equipments</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0VGLEzEQB_AgiterPvgFJIgngqzOZLPZ5LGtegpXLfZ8Dtk0e-bcbmqyC3d-enO0WtAHIZCH_JjM_IeQJwivERi-MRaYACFf3SMTrBgUQkp2n0wAoCxYDXBCTlO6BkAEFA_JCbKqKmvBJmQ1o0vfezOM0dG5D_ab39H1bRrclrYh0rducHbwoaehpTMXQwqd_-k2dG6s77ox0asuNP7Ke7rehejSI_KgNV1yjw_3lHx9_-5y8aG4-Hz-cTG7KEz-eSiEMtzl4yqssEYFjG9QcM6EU7WtGSBX2DZKmNZWFsGWDWPgZClU2XDByyl5sa-7i-HH6NKgtz5Z13Wmd2FMumZKyrqs_wtRMaYwJzUlz_6C12GMfR5CM6yl5LnVjF7ukc1RpOhavYt-a-KtRtB3y9C_l5Hp00O9sdm6zREews_g7ABMsqZro-mtT0fHcwRcquyKvfN5LTd_3k38rkWesNKXq7VeyuXi0-rLXN_Vfb73xqbjDP_09wtoQKhe</recordid><startdate>20030115</startdate><enddate>20030115</enddate><creator>Stratis-Cullum, Dimitra N.</creator><creator>Griffin, Guy D.</creator><creator>Mobley, Joel</creator><creator>Vass, Arpad A.</creator><creator>Vo-Dinh, Tuan</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030115</creationdate><title>A Miniature Biochip System for Detection of Aerosolized Bacillus globigii Spores</title><author>Stratis-Cullum, Dimitra N. ; Griffin, Guy D. ; Mobley, Joel ; Vass, Arpad A. ; Vo-Dinh, Tuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a537t-69a4ea4ee5151719024d164426e97c7201491fb96afc5c10c3b220e83693b4643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aerosols</topic><topic>Anthrax - prevention & control</topic><topic>Antigens, Bacterial - analysis</topic><topic>Bacillus - immunology</topic><topic>Bacillus anthracis</topic><topic>Bacillus globigii</topic><topic>Biological and medical sciences</topic><topic>Biological Warfare</topic><topic>biological warfare agents</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Enzyme-Linked Immunosorbent Assay - instrumentation</topic><topic>Enzyme-Linked Immunosorbent Assay - standards</topic><topic>Equipment Design</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lasers</topic><topic>Methods. Procedures. Technologies</topic><topic>Miniaturization</topic><topic>Spores, Bacterial - immunology</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stratis-Cullum, Dimitra N.</creatorcontrib><creatorcontrib>Griffin, Guy D.</creatorcontrib><creatorcontrib>Mobley, Joel</creatorcontrib><creatorcontrib>Vass, Arpad A.</creatorcontrib><creatorcontrib>Vo-Dinh, Tuan</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stratis-Cullum, Dimitra N.</au><au>Griffin, Guy D.</au><au>Mobley, Joel</au><au>Vass, Arpad A.</au><au>Vo-Dinh, Tuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Miniature Biochip System for Detection of Aerosolized Bacillus globigii Spores</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2003-01-15</date><risdate>2003</risdate><volume>75</volume><issue>2</issue><spage>275</spage><epage>280</epage><pages>275-280</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>The feasibility of using a novel detection scheme for the analysis of biological warfare agents is demonstrated using Bacillus globigii spores, a surrogate species for Bacillus anthracis. In this paper, a sensitive and selective enzyme-linked immunosorbent assay using a novel fluorogenic alkaline phosphatase substrate (dimethylacridinone phosphate) is combined with a compact biochip detection system, which includes a miniature diode laser for excitation. Detection of aerosolized spores was achieved by coupling the miniature system to a portable bioaerosol sampler, and the performance of the antibody-based recognition and enzyme amplification method was evaluated. The bioassay performance was found to be compatible with the air sampling device, and the enzymatic amplification was found to be an attractive amplification method for detection of low spore concentrations. The combined portable bioaerosol sampler and miniature biochip system detected 100 B. globigii spores, corresponding to 17 aerosolized spores/L of air. Moreover, the incorporation of the miniature diode laser with the self-contained biochip design allows for a compact system that is readily adaptable to field use. In addition, these studies have included investigations into the tradeoff between assay time and sensitivity.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>12553762</pmid><doi>10.1021/ac026068+</doi><tpages>6</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 2003-01, Vol.75 (2), p.275-280 |
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| subjects | Aerosols Anthrax - prevention & control Antigens, Bacterial - analysis Bacillus - immunology Bacillus anthracis Bacillus globigii Biological and medical sciences Biological Warfare biological warfare agents Biosensors Biotechnology Chemistry Enzyme-Linked Immunosorbent Assay - instrumentation Enzyme-Linked Immunosorbent Assay - standards Equipment Design Fundamental and applied biological sciences. Psychology Lasers Methods. Procedures. Technologies Miniaturization Spores, Bacterial - immunology Various methods and equipments |
| title | A Miniature Biochip System for Detection of Aerosolized Bacillus globigii Spores |
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