Identification of a putative intestinal stem cell and early lineage marker; musashi-1
There are few reliable markers for adult stem cells and none for those of the intestinal epithelium. Previously, indirect experimental approaches have predicted stem cell position and numbers. The Musashi-1 (Msi-1) gene encodes an RNA binding protein associated with asymmetric divisions in neural pr...
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| Veröffentlicht in: | Differentiation (London) 2003, Vol.71 (1), p.28-41 |
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| creator | Potten, Christopher S. Booth, Catherine Tudor, Gregory L. Booth, Dawn Brady, Gerard Hurley, Patricia Ashton, Gary Clarke, Robert Sakakibara, Shin-ichi Okano, Hideyuki |
| description | There are few reliable markers for adult stem cells and none for those of the intestinal epithelium. Previously, indirect experimental approaches have predicted stem cell position and numbers. The
Musashi-1 (Msi-1) gene encodes an RNA binding protein associated with asymmetric divisions in neural progenitor cells. Two-day-old, adult, and 4.5 h, 1-, 2-, 4- and 12-day post-irradiation samples of BDF1 mouse small intestine, together with some samples of mouse colon were stained with a rat monoclonal antibody to Musashi-1 (14 H-1). Min ( + / − ) mice with small intestinal adenomas of varying sizes were also analysed. Samples of human small and large bowel were also studied but the antibody staining was weak. Musashi-1 expression was observed using immunohistochemistry in neonatal, adult, and regenerating crypts with a staining pattern consistent with the predicted number and distribution of early lineage cells including the functional stem cells in these situations. Early dysplastic crypts and adenomas were also strongly Musashi-1 positive.
In situ hybridization studies showed similar expression patterns for the Musashi mRNA and real-time quantitative RT-PCR showed dramatically more Msi-1 mRNA expression in Min tumours compared with adjacent normal tissue. These observations suggest that Musashi-1 is a marker of stem and early lineage progenitor cells in murine intestinal tissue. |
| doi_str_mv | 10.1046/j.1432-0436.2003.700603.x |
| format | Article |
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Musashi-1 (Msi-1) gene encodes an RNA binding protein associated with asymmetric divisions in neural progenitor cells. Two-day-old, adult, and 4.5 h, 1-, 2-, 4- and 12-day post-irradiation samples of BDF1 mouse small intestine, together with some samples of mouse colon were stained with a rat monoclonal antibody to Musashi-1 (14 H-1). Min ( + / − ) mice with small intestinal adenomas of varying sizes were also analysed. Samples of human small and large bowel were also studied but the antibody staining was weak. Musashi-1 expression was observed using immunohistochemistry in neonatal, adult, and regenerating crypts with a staining pattern consistent with the predicted number and distribution of early lineage cells including the functional stem cells in these situations. Early dysplastic crypts and adenomas were also strongly Musashi-1 positive.
In situ hybridization studies showed similar expression patterns for the Musashi mRNA and real-time quantitative RT-PCR showed dramatically more Msi-1 mRNA expression in Min tumours compared with adjacent normal tissue. These observations suggest that Musashi-1 is a marker of stem and early lineage progenitor cells in murine intestinal tissue.</description><identifier>ISSN: 0301-4681</identifier><identifier>EISSN: 1432-0436</identifier><identifier>DOI: 10.1046/j.1432-0436.2003.700603.x</identifier><identifier>PMID: 12558601</identifier><language>eng</language><publisher>Berlin/Wien: Elsevier B.V</publisher><subject>Adult ; Aged ; Animals ; Biological and medical sciences ; Biomarkers ; Cell differentiation, maturation, development, hematopoiesis ; Cell Lineage ; Cell physiology ; Child ; clonogenic cells ; Fundamental and applied biological sciences. Psychology ; Gamma Rays ; Humans ; Intestinal Mucosa - cytology ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - radiation effects ; Intestinal Neoplasms - metabolism ; Intestinal Neoplasms - pathology ; Male ; Mice ; Molecular and cellular biology ; Musashi-1 ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; small intestine ; stem cell marker ; stem cells ; Stem Cells - cytology ; Stem Cells - metabolism</subject><ispartof>Differentiation (London), 2003, Vol.71 (1), p.28-41</ispartof><rights>2003 International Society of Differentiation</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5263-3a42b5f88ae078a454dbfee6a4bbc262933bf7b944ae85dfd45efe24bcf2d40b3</citedby><cites>FETCH-LOGICAL-c5263-3a42b5f88ae078a454dbfee6a4bbc262933bf7b944ae85dfd45efe24bcf2d40b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1432-0436.2003.700603.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0301468109602644$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,1411,3537,4010,27900,27901,27902,45550,45551,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14433878$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12558601$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Potten, Christopher S.</creatorcontrib><creatorcontrib>Booth, Catherine</creatorcontrib><creatorcontrib>Tudor, Gregory L.</creatorcontrib><creatorcontrib>Booth, Dawn</creatorcontrib><creatorcontrib>Brady, Gerard</creatorcontrib><creatorcontrib>Hurley, Patricia</creatorcontrib><creatorcontrib>Ashton, Gary</creatorcontrib><creatorcontrib>Clarke, Robert</creatorcontrib><creatorcontrib>Sakakibara, Shin-ichi</creatorcontrib><creatorcontrib>Okano, Hideyuki</creatorcontrib><title>Identification of a putative intestinal stem cell and early lineage marker; musashi-1</title><title>Differentiation (London)</title><addtitle>Differentiation</addtitle><description>There are few reliable markers for adult stem cells and none for those of the intestinal epithelium. Previously, indirect experimental approaches have predicted stem cell position and numbers. The
Musashi-1 (Msi-1) gene encodes an RNA binding protein associated with asymmetric divisions in neural progenitor cells. Two-day-old, adult, and 4.5 h, 1-, 2-, 4- and 12-day post-irradiation samples of BDF1 mouse small intestine, together with some samples of mouse colon were stained with a rat monoclonal antibody to Musashi-1 (14 H-1). Min ( + / − ) mice with small intestinal adenomas of varying sizes were also analysed. Samples of human small and large bowel were also studied but the antibody staining was weak. Musashi-1 expression was observed using immunohistochemistry in neonatal, adult, and regenerating crypts with a staining pattern consistent with the predicted number and distribution of early lineage cells including the functional stem cells in these situations. Early dysplastic crypts and adenomas were also strongly Musashi-1 positive.
In situ hybridization studies showed similar expression patterns for the Musashi mRNA and real-time quantitative RT-PCR showed dramatically more Msi-1 mRNA expression in Min tumours compared with adjacent normal tissue. These observations suggest that Musashi-1 is a marker of stem and early lineage progenitor cells in murine intestinal tissue.</description><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell Lineage</subject><subject>Cell physiology</subject><subject>Child</subject><subject>clonogenic cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gamma Rays</subject><subject>Humans</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - radiation effects</subject><subject>Intestinal Neoplasms - metabolism</subject><subject>Intestinal Neoplasms - pathology</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Musashi-1</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>small intestine</subject><subject>stem cell marker</subject><subject>stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><issn>0301-4681</issn><issn>1432-0436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtvEzEURq2Kqk0LfwGZBewm9Ws8jtiAAoVIldjQteXHNTjMI9gzpfn3eDRRu-3qyvK5nz8fhN5RsqZEyJv9mgrOKiK4XDNC-LohRJbxeIZWTzev0IpwQishFb1EVznvCSFKMnqBLimrayUJXaH7nYd-jCE6M8ahx0PABh-msZweAMd-hDzG3rQ4j9BhB22LTe8xmNQecRt7ML8Adyb9gfQRd1M2-Xes6Gt0Hkyb4c1pXqP7268_t9-rux_fdtvPd5WrmeQVN4LZOihlgDTKiFp4GwCkEdY6JtmGcxsauxHCgKp98KKGAExYF5gXxPJr9GHJPaTh71Sq6i7muaTpYZiybthGKarqAm4W0KUh5wRBH1IstY-aEj071Xs9m9OzOT071YtT_Vh2354emWwH_nnzJLEA70-Ayc60IZnexfzMCcG5alThPi3cv9jC8eUN9JfdbUMJJbxEbJcIKFYfIiSdXYTegY8J3Kj9EF_wo_-GTqjn</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Potten, Christopher S.</creator><creator>Booth, Catherine</creator><creator>Tudor, Gregory L.</creator><creator>Booth, Dawn</creator><creator>Brady, Gerard</creator><creator>Hurley, Patricia</creator><creator>Ashton, Gary</creator><creator>Clarke, Robert</creator><creator>Sakakibara, Shin-ichi</creator><creator>Okano, Hideyuki</creator><general>Elsevier B.V</general><general>Blackwell Wissenschafts‐Verlag</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Identification of a putative intestinal stem cell and early lineage marker; musashi-1</title><author>Potten, Christopher S. ; Booth, Catherine ; Tudor, Gregory L. ; Booth, Dawn ; Brady, Gerard ; Hurley, Patricia ; Ashton, Gary ; Clarke, Robert ; Sakakibara, Shin-ichi ; Okano, Hideyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5263-3a42b5f88ae078a454dbfee6a4bbc262933bf7b944ae85dfd45efe24bcf2d40b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell Lineage</topic><topic>Cell physiology</topic><topic>Child</topic><topic>clonogenic cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gamma Rays</topic><topic>Humans</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - radiation effects</topic><topic>Intestinal Neoplasms - metabolism</topic><topic>Intestinal Neoplasms - pathology</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Musashi-1</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>small intestine</topic><topic>stem cell marker</topic><topic>stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Potten, Christopher S.</creatorcontrib><creatorcontrib>Booth, Catherine</creatorcontrib><creatorcontrib>Tudor, Gregory L.</creatorcontrib><creatorcontrib>Booth, Dawn</creatorcontrib><creatorcontrib>Brady, Gerard</creatorcontrib><creatorcontrib>Hurley, Patricia</creatorcontrib><creatorcontrib>Ashton, Gary</creatorcontrib><creatorcontrib>Clarke, Robert</creatorcontrib><creatorcontrib>Sakakibara, Shin-ichi</creatorcontrib><creatorcontrib>Okano, Hideyuki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Differentiation (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Potten, Christopher S.</au><au>Booth, Catherine</au><au>Tudor, Gregory L.</au><au>Booth, Dawn</au><au>Brady, Gerard</au><au>Hurley, Patricia</au><au>Ashton, Gary</au><au>Clarke, Robert</au><au>Sakakibara, Shin-ichi</au><au>Okano, Hideyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a putative intestinal stem cell and early lineage marker; musashi-1</atitle><jtitle>Differentiation (London)</jtitle><addtitle>Differentiation</addtitle><date>2003</date><risdate>2003</risdate><volume>71</volume><issue>1</issue><spage>28</spage><epage>41</epage><pages>28-41</pages><issn>0301-4681</issn><eissn>1432-0436</eissn><abstract>There are few reliable markers for adult stem cells and none for those of the intestinal epithelium. Previously, indirect experimental approaches have predicted stem cell position and numbers. The
Musashi-1 (Msi-1) gene encodes an RNA binding protein associated with asymmetric divisions in neural progenitor cells. Two-day-old, adult, and 4.5 h, 1-, 2-, 4- and 12-day post-irradiation samples of BDF1 mouse small intestine, together with some samples of mouse colon were stained with a rat monoclonal antibody to Musashi-1 (14 H-1). Min ( + / − ) mice with small intestinal adenomas of varying sizes were also analysed. Samples of human small and large bowel were also studied but the antibody staining was weak. Musashi-1 expression was observed using immunohistochemistry in neonatal, adult, and regenerating crypts with a staining pattern consistent with the predicted number and distribution of early lineage cells including the functional stem cells in these situations. Early dysplastic crypts and adenomas were also strongly Musashi-1 positive.
In situ hybridization studies showed similar expression patterns for the Musashi mRNA and real-time quantitative RT-PCR showed dramatically more Msi-1 mRNA expression in Min tumours compared with adjacent normal tissue. These observations suggest that Musashi-1 is a marker of stem and early lineage progenitor cells in murine intestinal tissue.</abstract><cop>Berlin/Wien</cop><pub>Elsevier B.V</pub><pmid>12558601</pmid><doi>10.1046/j.1432-0436.2003.700603.x</doi><tpages>14</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); Wiley Online Library Journals Frontfile Complete |
| subjects | Adult Aged Animals Biological and medical sciences Biomarkers Cell differentiation, maturation, development, hematopoiesis Cell Lineage Cell physiology Child clonogenic cells Fundamental and applied biological sciences. Psychology Gamma Rays Humans Intestinal Mucosa - cytology Intestinal Mucosa - metabolism Intestinal Mucosa - radiation effects Intestinal Neoplasms - metabolism Intestinal Neoplasms - pathology Male Mice Molecular and cellular biology Musashi-1 Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism small intestine stem cell marker stem cells Stem Cells - cytology Stem Cells - metabolism |
| title | Identification of a putative intestinal stem cell and early lineage marker; musashi-1 |
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