Effect of major nutrients on podophyllotoxin production in Podophyllum hexandrum suspension cultures
The effect of major medium ingredients (sugar, nitrogen source and phosphate) in Podophyllum hexandrum suspension cultures was investigated in order to increase the production of podophyllotoxin, the raw material in the synthesis of anticancer drugs. Amongst B5, Eriksson, MS, Nitsch, Street and Whit...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2003, Vol.60 (5), p.541-546 |
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| creator | Chattopadhyay, S Mehra, R.S Srivastava, A.K Bhojwani, S.S Bisaria, V.S |
| description | The effect of major medium ingredients (sugar, nitrogen source and phosphate) in Podophyllum hexandrum suspension cultures was investigated in order to increase the production of podophyllotoxin, the raw material in the synthesis of anticancer drugs. Amongst B5, Eriksson, MS, Nitsch, Street and White's medium, MS medium resulted in high growth and podophyllotoxin accumulation. The optimum level of nitrogen was found to be 60 mM, with a combination of ammonium salts and nitrate in the ratio of 1:2. The highest level of podophyllotoxin was obtained at 60 g glucose/l and at 1.25 mM phosphate after 30 days. Statistical design was adopted to determine the optimum levels of the parameters for cell growth and podophyllotoxin production. |
| doi_str_mv | 10.1007/s00253-002-1149-7 |
| format | Article |
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Amongst B5, Eriksson, MS, Nitsch, Street and White's medium, MS medium resulted in high growth and podophyllotoxin accumulation. The optimum level of nitrogen was found to be 60 mM, with a combination of ammonium salts and nitrate in the ratio of 1:2. The highest level of podophyllotoxin was obtained at 60 g glucose/l and at 1.25 mM phosphate after 30 days. Statistical design was adopted to determine the optimum levels of the parameters for cell growth and podophyllotoxin production.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-002-1149-7</identifier><identifier>PMID: 12536253</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Ammonium ; Ammonium salts ; antineoplastic agents ; Antineoplastic Agents, Phytogenic - biosynthesis ; Antineoplastic drugs. Cytokines ; Biological and medical sciences ; Biotechnology ; Cell culture ; Cell growth ; Cells, Cultured ; Chemical products ; Culture Media ; Experiments ; Fundamental and applied biological sciences. Psychology ; Glucose ; Glucose - metabolism ; Health. Pharmaceutical industry ; Industrial applications and implications. Economical aspects ; ingredients ; Metabolites ; Nitrates ; Nitrogen ; Nitrogen - metabolism ; Nutrients ; Optimization ; phosphates ; Phosphates - metabolism ; Plant Roots - cytology ; Plants, Medicinal ; podophyllotoxin ; Podophyllotoxin - analysis ; Podophyllotoxin - biosynthesis ; Podophyllum - cytology ; Podophyllum - growth & development ; Podophyllum - metabolism ; Podophyllum hexandrum ; Production of active biomolecules ; raw materials ; Reproducibility ; Studies</subject><ispartof>Applied microbiology and biotechnology, 2003, Vol.60 (5), p.541-546</ispartof><rights>2003 INIST-CNRS</rights><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-f9a8a4979e5d77ab0b6c91393342792f57465c035c0956649dba477b6c2d21f23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,4012,27906,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14592343$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12536253$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chattopadhyay, S</creatorcontrib><creatorcontrib>Mehra, R.S</creatorcontrib><creatorcontrib>Srivastava, A.K</creatorcontrib><creatorcontrib>Bhojwani, S.S</creatorcontrib><creatorcontrib>Bisaria, V.S</creatorcontrib><title>Effect of major nutrients on podophyllotoxin production in Podophyllum hexandrum suspension cultures</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>The effect of major medium ingredients (sugar, nitrogen source and phosphate) in Podophyllum hexandrum suspension cultures was investigated in order to increase the production of podophyllotoxin, the raw material in the synthesis of anticancer drugs. Amongst B5, Eriksson, MS, Nitsch, Street and White's medium, MS medium resulted in high growth and podophyllotoxin accumulation. The optimum level of nitrogen was found to be 60 mM, with a combination of ammonium salts and nitrate in the ratio of 1:2. The highest level of podophyllotoxin was obtained at 60 g glucose/l and at 1.25 mM phosphate after 30 days. Statistical design was adopted to determine the optimum levels of the parameters for cell growth and podophyllotoxin production.</description><subject>Ammonium</subject><subject>Ammonium salts</subject><subject>antineoplastic agents</subject><subject>Antineoplastic Agents, Phytogenic - biosynthesis</subject><subject>Antineoplastic drugs. Cytokines</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Cell growth</subject><subject>Cells, Cultured</subject><subject>Chemical products</subject><subject>Culture Media</subject><subject>Experiments</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>Health. Pharmaceutical industry</subject><subject>Industrial applications and implications. 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Cytokines</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell culture</topic><topic>Cell growth</topic><topic>Cells, Cultured</topic><topic>Chemical products</topic><topic>Culture Media</topic><topic>Experiments</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>Health. Pharmaceutical industry</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>ingredients</topic><topic>Metabolites</topic><topic>Nitrates</topic><topic>Nitrogen</topic><topic>Nitrogen - metabolism</topic><topic>Nutrients</topic><topic>Optimization</topic><topic>phosphates</topic><topic>Phosphates - metabolism</topic><topic>Plant Roots - cytology</topic><topic>Plants, Medicinal</topic><topic>podophyllotoxin</topic><topic>Podophyllotoxin - analysis</topic><topic>Podophyllotoxin - biosynthesis</topic><topic>Podophyllum - cytology</topic><topic>Podophyllum - growth & development</topic><topic>Podophyllum - metabolism</topic><topic>Podophyllum hexandrum</topic><topic>Production of active biomolecules</topic><topic>raw materials</topic><topic>Reproducibility</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chattopadhyay, S</creatorcontrib><creatorcontrib>Mehra, R.S</creatorcontrib><creatorcontrib>Srivastava, A.K</creatorcontrib><creatorcontrib>Bhojwani, S.S</creatorcontrib><creatorcontrib>Bisaria, V.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chattopadhyay, S</au><au>Mehra, R.S</au><au>Srivastava, A.K</au><au>Bhojwani, S.S</au><au>Bisaria, V.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of major nutrients on podophyllotoxin production in Podophyllum hexandrum suspension cultures</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2003</date><risdate>2003</risdate><volume>60</volume><issue>5</issue><spage>541</spage><epage>546</epage><pages>541-546</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>The effect of major medium ingredients (sugar, nitrogen source and phosphate) in Podophyllum hexandrum suspension cultures was investigated in order to increase the production of podophyllotoxin, the raw material in the synthesis of anticancer drugs. Amongst B5, Eriksson, MS, Nitsch, Street and White's medium, MS medium resulted in high growth and podophyllotoxin accumulation. The optimum level of nitrogen was found to be 60 mM, with a combination of ammonium salts and nitrate in the ratio of 1:2. The highest level of podophyllotoxin was obtained at 60 g glucose/l and at 1.25 mM phosphate after 30 days. Statistical design was adopted to determine the optimum levels of the parameters for cell growth and podophyllotoxin production.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>12536253</pmid><doi>10.1007/s00253-002-1149-7</doi><tpages>6</tpages></addata></record> |
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| subjects | Ammonium Ammonium salts antineoplastic agents Antineoplastic Agents, Phytogenic - biosynthesis Antineoplastic drugs. Cytokines Biological and medical sciences Biotechnology Cell culture Cell growth Cells, Cultured Chemical products Culture Media Experiments Fundamental and applied biological sciences. Psychology Glucose Glucose - metabolism Health. Pharmaceutical industry Industrial applications and implications. Economical aspects ingredients Metabolites Nitrates Nitrogen Nitrogen - metabolism Nutrients Optimization phosphates Phosphates - metabolism Plant Roots - cytology Plants, Medicinal podophyllotoxin Podophyllotoxin - analysis Podophyllotoxin - biosynthesis Podophyllum - cytology Podophyllum - growth & development Podophyllum - metabolism Podophyllum hexandrum Production of active biomolecules raw materials Reproducibility Studies |
| title | Effect of major nutrients on podophyllotoxin production in Podophyllum hexandrum suspension cultures |
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