Occurrence and localization of uroguanylin in the aging human prostate
Uroguanylin, a peptide hormone highly expressed in the gastrointestinal tract, is implicated in the regulation of epithelial salt and water transport processes. Since little is known about a possible role of uroguanylin in the reproductive system, we investigated for the first time the occurrence of...
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| Veröffentlicht in: | Histochemistry and cell biology 2003-01, Vol.119 (1), p.69-76 |
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| creator | Maake, Caroline Auf der Maur, Franziska Jovanovic, Katarina Reinecke, Manfred Hauri, Dieter John, Hubert |
| description | Uroguanylin, a peptide hormone highly expressed in the gastrointestinal tract, is implicated in the regulation of epithelial salt and water transport processes. Since little is known about a possible role of uroguanylin in the reproductive system, we investigated for the first time the occurrence of this peptide in the human prostate using specimens of benign prostatic hyperplasia. Northern blot analyses detected a single uroguanylin transcript of approximately 600 bp in prostate RNA. The uroguanylin expression was further investigated by reverse transcriptase polymerase chain reaction of prostate RNA with uroguanylin-specific primers. Sequencing of the fragments obtained indicated the presence of a uroguanylin molecule with a sequence identical to its intestinal counterpart. Furthermore, in situ hybridization and immunohistochemistry revealed that uroguanylin mRNA and peptide are confined to epithelial cells of the prostate glands. Comparison with the distribution pattern of immunoreactivity for prostate-specific antigen (PSA) showed a high degree of colocalization of uroguanylin- and PSA-immunoreactive cells. In addition, by western blotting techniques we detected the presence of high molecular weight uroguanylin-immunoreactive material in prostatic fluid. In conclusion, our study indicates that the human prostate glands synthesize and secrete (pro-)uroguanylin. We hypothesize that this hormone may play a novel role in the male reproductive tract. |
| doi_str_mv | 10.1007/s00418-002-0490-3 |
| format | Article |
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Since little is known about a possible role of uroguanylin in the reproductive system, we investigated for the first time the occurrence of this peptide in the human prostate using specimens of benign prostatic hyperplasia. Northern blot analyses detected a single uroguanylin transcript of approximately 600 bp in prostate RNA. The uroguanylin expression was further investigated by reverse transcriptase polymerase chain reaction of prostate RNA with uroguanylin-specific primers. Sequencing of the fragments obtained indicated the presence of a uroguanylin molecule with a sequence identical to its intestinal counterpart. Furthermore, in situ hybridization and immunohistochemistry revealed that uroguanylin mRNA and peptide are confined to epithelial cells of the prostate glands. Comparison with the distribution pattern of immunoreactivity for prostate-specific antigen (PSA) showed a high degree of colocalization of uroguanylin- and PSA-immunoreactive cells. In addition, by western blotting techniques we detected the presence of high molecular weight uroguanylin-immunoreactive material in prostatic fluid. In conclusion, our study indicates that the human prostate glands synthesize and secrete (pro-)uroguanylin. We hypothesize that this hormone may play a novel role in the male reproductive tract.</description><identifier>ISSN: 0948-6143</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/s00418-002-0490-3</identifier><identifier>PMID: 12548407</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Aged ; Aging - physiology ; Blotting, Northern ; Humans ; Immunoenzyme Techniques ; In Situ Hybridization ; Male ; Middle Aged ; Natriuretic Peptides ; Peptides - analysis ; Peptides - genetics ; Peptides - metabolism ; Prostate - chemistry ; Prostate - metabolism ; Prostate - pathology ; Prostate-Specific Antigen - analysis ; Prostate-Specific Antigen - metabolism ; Prostatic Hyperplasia - metabolism ; Prostatic Hyperplasia - pathology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Sequence Analysis, RNA</subject><ispartof>Histochemistry and cell biology, 2003-01, Vol.119 (1), p.69-76</ispartof><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c324t-22376606642ba58ec82e85ea5d73cc0eb847e0bd42b565a0af554c0d5e37079d3</citedby><cites>FETCH-LOGICAL-c324t-22376606642ba58ec82e85ea5d73cc0eb847e0bd42b565a0af554c0d5e37079d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12548407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maake, Caroline</creatorcontrib><creatorcontrib>Auf der Maur, Franziska</creatorcontrib><creatorcontrib>Jovanovic, Katarina</creatorcontrib><creatorcontrib>Reinecke, Manfred</creatorcontrib><creatorcontrib>Hauri, Dieter</creatorcontrib><creatorcontrib>John, Hubert</creatorcontrib><title>Occurrence and localization of uroguanylin in the aging human prostate</title><title>Histochemistry and cell biology</title><addtitle>Histochem Cell Biol</addtitle><description>Uroguanylin, a peptide hormone highly expressed in the gastrointestinal tract, is implicated in the regulation of epithelial salt and water transport processes. Since little is known about a possible role of uroguanylin in the reproductive system, we investigated for the first time the occurrence of this peptide in the human prostate using specimens of benign prostatic hyperplasia. Northern blot analyses detected a single uroguanylin transcript of approximately 600 bp in prostate RNA. The uroguanylin expression was further investigated by reverse transcriptase polymerase chain reaction of prostate RNA with uroguanylin-specific primers. Sequencing of the fragments obtained indicated the presence of a uroguanylin molecule with a sequence identical to its intestinal counterpart. Furthermore, in situ hybridization and immunohistochemistry revealed that uroguanylin mRNA and peptide are confined to epithelial cells of the prostate glands. Comparison with the distribution pattern of immunoreactivity for prostate-specific antigen (PSA) showed a high degree of colocalization of uroguanylin- and PSA-immunoreactive cells. In addition, by western blotting techniques we detected the presence of high molecular weight uroguanylin-immunoreactive material in prostatic fluid. In conclusion, our study indicates that the human prostate glands synthesize and secrete (pro-)uroguanylin. We hypothesize that this hormone may play a novel role in the male reproductive tract.</description><subject>Aged</subject><subject>Aging - physiology</subject><subject>Blotting, Northern</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Natriuretic Peptides</subject><subject>Peptides - analysis</subject><subject>Peptides - genetics</subject><subject>Peptides - metabolism</subject><subject>Prostate - chemistry</subject><subject>Prostate - metabolism</subject><subject>Prostate - pathology</subject><subject>Prostate-Specific Antigen - analysis</subject><subject>Prostate-Specific Antigen - metabolism</subject><subject>Prostatic Hyperplasia - metabolism</subject><subject>Prostatic Hyperplasia - pathology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Analysis, RNA</subject><issn>0948-6143</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkM9LwzAUx4Mobk7_AC9SPHirvvxq0qMMp8JgFwVvIU3TraNNZ9Ic5l9vxgaCEAjkfb7vvXwQusXwiAHEUwBgWOYAJAdWQk7P0BQzSnKMy69zNIWSybxILxN0FcIWAPOSkEs0wYQzyUBM0WJlTPTeOmMz7eqsG4zu2h89toPLhiaLflhH7fZd67J0xk3C1q1bZ5vYa5ft_BBGPdprdNHoLtib0z1Dn4uXj_lbvly9vs-fl7mhhI05IVQUBRQFI5Xm0hpJrORW81pQY8BWkgkLVZ3KvOAadMM5M1BzSwWIsqYz9HDsmwZ_RxtG1bfB2K7Tzg4xKEFKwaQkCbz_B26H6F3aTRHMDx5KniB8hEz6RvC2UTvf9trvFQZ1MKyOhlUyrA6GFU2Zu1PjWPW2_kuclNJfEhF1uA</recordid><startdate>200301</startdate><enddate>200301</enddate><creator>Maake, Caroline</creator><creator>Auf der Maur, Franziska</creator><creator>Jovanovic, Katarina</creator><creator>Reinecke, Manfred</creator><creator>Hauri, Dieter</creator><creator>John, Hubert</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>200301</creationdate><title>Occurrence and localization of uroguanylin in the aging human prostate</title><author>Maake, Caroline ; Auf der Maur, Franziska ; Jovanovic, Katarina ; Reinecke, Manfred ; Hauri, Dieter ; John, Hubert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c324t-22376606642ba58ec82e85ea5d73cc0eb847e0bd42b565a0af554c0d5e37079d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aged</topic><topic>Aging - physiology</topic><topic>Blotting, Northern</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Natriuretic Peptides</topic><topic>Peptides - analysis</topic><topic>Peptides - genetics</topic><topic>Peptides - metabolism</topic><topic>Prostate - chemistry</topic><topic>Prostate - metabolism</topic><topic>Prostate - pathology</topic><topic>Prostate-Specific Antigen - analysis</topic><topic>Prostate-Specific Antigen - metabolism</topic><topic>Prostatic Hyperplasia - metabolism</topic><topic>Prostatic Hyperplasia - pathology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Analysis, RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maake, Caroline</creatorcontrib><creatorcontrib>Auf der Maur, Franziska</creatorcontrib><creatorcontrib>Jovanovic, Katarina</creatorcontrib><creatorcontrib>Reinecke, Manfred</creatorcontrib><creatorcontrib>Hauri, Dieter</creatorcontrib><creatorcontrib>John, Hubert</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Histochemistry and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maake, Caroline</au><au>Auf der Maur, Franziska</au><au>Jovanovic, Katarina</au><au>Reinecke, Manfred</au><au>Hauri, Dieter</au><au>John, Hubert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence and localization of uroguanylin in the aging human prostate</atitle><jtitle>Histochemistry and cell biology</jtitle><addtitle>Histochem Cell Biol</addtitle><date>2003-01</date><risdate>2003</risdate><volume>119</volume><issue>1</issue><spage>69</spage><epage>76</epage><pages>69-76</pages><issn>0948-6143</issn><eissn>1432-119X</eissn><abstract>Uroguanylin, a peptide hormone highly expressed in the gastrointestinal tract, is implicated in the regulation of epithelial salt and water transport processes. Since little is known about a possible role of uroguanylin in the reproductive system, we investigated for the first time the occurrence of this peptide in the human prostate using specimens of benign prostatic hyperplasia. Northern blot analyses detected a single uroguanylin transcript of approximately 600 bp in prostate RNA. The uroguanylin expression was further investigated by reverse transcriptase polymerase chain reaction of prostate RNA with uroguanylin-specific primers. Sequencing of the fragments obtained indicated the presence of a uroguanylin molecule with a sequence identical to its intestinal counterpart. Furthermore, in situ hybridization and immunohistochemistry revealed that uroguanylin mRNA and peptide are confined to epithelial cells of the prostate glands. Comparison with the distribution pattern of immunoreactivity for prostate-specific antigen (PSA) showed a high degree of colocalization of uroguanylin- and PSA-immunoreactive cells. In addition, by western blotting techniques we detected the presence of high molecular weight uroguanylin-immunoreactive material in prostatic fluid. In conclusion, our study indicates that the human prostate glands synthesize and secrete (pro-)uroguanylin. We hypothesize that this hormone may play a novel role in the male reproductive tract.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>12548407</pmid><doi>10.1007/s00418-002-0490-3</doi><tpages>8</tpages></addata></record> |
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| ispartof | Histochemistry and cell biology, 2003-01, Vol.119 (1), p.69-76 |
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| subjects | Aged Aging - physiology Blotting, Northern Humans Immunoenzyme Techniques In Situ Hybridization Male Middle Aged Natriuretic Peptides Peptides - analysis Peptides - genetics Peptides - metabolism Prostate - chemistry Prostate - metabolism Prostate - pathology Prostate-Specific Antigen - analysis Prostate-Specific Antigen - metabolism Prostatic Hyperplasia - metabolism Prostatic Hyperplasia - pathology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sequence Analysis, RNA |
| title | Occurrence and localization of uroguanylin in the aging human prostate |
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