Expression and localization of carbonic anhydrase in bovine mammary gland and secretion in milk
Little attention has been paid to carbonic anhydrase VI (CA VI), a secretory type isozyme, in the bovine mammary gland, although the gland is an important exocrine gland and CA VI is known to localize in exocrine glands such as salivary and lacrimal glands in various animal species. In the present s...
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| Veröffentlicht in: | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2003-02, Vol.134 (2), p.349-354 |
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| container_title | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology |
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| creator | Kitade, Keitaro Nishita, Toshiho Yamato, Mitsugu Sakamoto, Kazuhito Hagino, Akihiko Katoh, Kazuo Obara, Yoshiaki |
| description | Little attention has been paid to carbonic anhydrase VI (CA VI), a secretory type isozyme, in the bovine mammary gland, although the gland is an important exocrine gland and CA VI is known to localize in exocrine glands such as salivary and lacrimal glands in various animal species. In the present study mRNA expression and protein localization of CA VI in isolated gland tissues and in cloned epithelial cells from the mammary gland of Holstein cows (
Bos taurus) were observed by reverse transcript polymerase chain reaction and immunocytochemistry. Also, changes of CA VI concentrations in milk were measured for 2 months postpartum by an enzyme-linked immunosorbent assay. CA VI gene expression was detected in the gland tissues and epithelial cells, and CA VI protein was localized in the cytoplasm of the epithelial cells. Colostrum contained the highest concentration of CA VI protein (100 ng/ml), decreasing in an exponential manner (
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| doi_str_mv | 10.1016/S1095-6433(02)00268-4 |
| format | Article |
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Bos taurus) were observed by reverse transcript polymerase chain reaction and immunocytochemistry. Also, changes of CA VI concentrations in milk were measured for 2 months postpartum by an enzyme-linked immunosorbent assay. CA VI gene expression was detected in the gland tissues and epithelial cells, and CA VI protein was localized in the cytoplasm of the epithelial cells. Colostrum contained the highest concentration of CA VI protein (100 ng/ml), decreasing in an exponential manner (
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Bos taurus) were observed by reverse transcript polymerase chain reaction and immunocytochemistry. Also, changes of CA VI concentrations in milk were measured for 2 months postpartum by an enzyme-linked immunosorbent assay. CA VI gene expression was detected in the gland tissues and epithelial cells, and CA VI protein was localized in the cytoplasm of the epithelial cells. Colostrum contained the highest concentration of CA VI protein (100 ng/ml), decreasing in an exponential manner (
P<0.001). We conclude that bovine mammary epithelial cells synthesize and secrete CA VI in colostrum at higher concentration than in normal milk, implying its role to compensate for low CA VI secretion in neonatal calves.</description><subject>Animals</subject><subject>Carbonic anhydrase VI</subject><subject>Carbonic Anhydrases - analysis</subject><subject>Carbonic Anhydrases - genetics</subject><subject>Carbonic Anhydrases - metabolism</subject><subject>Carbonic Anhydrases - secretion</subject><subject>Cattle - physiology</subject><subject>Clone Cells</subject><subject>Colostrum</subject><subject>Colostrum - chemistry</subject><subject>Cow</subject><subject>DNA, Complementary - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epithelial Cells - enzymology</subject><subject>Female</subject><subject>Immunocytochemistry</subject><subject>Immunohistochemistry</subject><subject>Lactation</subject><subject>Mammary epithelial cells</subject><subject>Mammary Glands, Animal - cytology</subject><subject>Mammary Glands, Animal - enzymology</subject><subject>Milk - chemistry</subject><subject>Neonate</subject><subject>Osmolar Concentration</subject><subject>Parotid Gland - enzymology</subject><subject>Protein Subunits - analysis</subject><subject>Protein Subunits - genetics</subject><subject>Protein Subunits - metabolism</subject><subject>Protein Subunits - secretion</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RT-PCR</subject><subject>Secretion</subject><subject>Tissue Distribution</subject><issn>1095-6433</issn><issn>1531-4332</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PAyEQhonRWK3-BHVPRg-rwLIfPRnT1I-kiYfaMwF2qOjuUmHbWH-9bLfGoyQTYHjeYeZF6IzgG4JJdjsjeJTGGUuSK0yvMaZZEbM9dETShMQhS_fD-RcZoGPv33FYjLBDNCA0ZTnN2BHik6-lA--NbSLRlFFllajMt2i7hNWREk7axqjw-LYpnfAQmSaSdm0aiGpR18JtokXVSbvwoBxstYGqTfVxgg60qDyc7vYhmj9MXsdP8fTl8Xl8P40VS0kbp0WhaZZS0AyYLCXRI01xmeEEaCJAY62ASJGXBStkkhNMZZbrME3RXbVKhuiyr7t09nMFvuW18Qqq0BnYlec5HeUsS3AA0x5UznrvQPOlM90UnGDeOcu3zvLONo4p3zrLWdCd7z5YyRrKP9XOygBc9IAWlouFM57PZzQUxCFIkY8CcdcTEIxYG3DcKwONgtI4UC0vrfmniR_4CJJ0</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>Kitade, Keitaro</creator><creator>Nishita, Toshiho</creator><creator>Yamato, Mitsugu</creator><creator>Sakamoto, Kazuhito</creator><creator>Hagino, Akihiko</creator><creator>Katoh, Kazuo</creator><creator>Obara, Yoshiaki</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030201</creationdate><title>Expression and localization of carbonic anhydrase in bovine mammary gland and secretion in milk</title><author>Kitade, Keitaro ; Nishita, Toshiho ; Yamato, Mitsugu ; Sakamoto, Kazuhito ; Hagino, Akihiko ; Katoh, Kazuo ; Obara, Yoshiaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-588f2652ef4e4bdb1f9f20d603e23aef0fce1ba7d848b37102b67f04188b37fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Carbonic anhydrase VI</topic><topic>Carbonic Anhydrases - analysis</topic><topic>Carbonic Anhydrases - genetics</topic><topic>Carbonic Anhydrases - metabolism</topic><topic>Carbonic Anhydrases - secretion</topic><topic>Cattle - physiology</topic><topic>Clone Cells</topic><topic>Colostrum</topic><topic>Colostrum - chemistry</topic><topic>Cow</topic><topic>DNA, Complementary - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epithelial Cells - enzymology</topic><topic>Female</topic><topic>Immunocytochemistry</topic><topic>Immunohistochemistry</topic><topic>Lactation</topic><topic>Mammary epithelial cells</topic><topic>Mammary Glands, Animal - cytology</topic><topic>Mammary Glands, Animal - enzymology</topic><topic>Milk - chemistry</topic><topic>Neonate</topic><topic>Osmolar Concentration</topic><topic>Parotid Gland - enzymology</topic><topic>Protein Subunits - analysis</topic><topic>Protein Subunits - genetics</topic><topic>Protein Subunits - metabolism</topic><topic>Protein Subunits - secretion</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RT-PCR</topic><topic>Secretion</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitade, Keitaro</creatorcontrib><creatorcontrib>Nishita, Toshiho</creatorcontrib><creatorcontrib>Yamato, Mitsugu</creatorcontrib><creatorcontrib>Sakamoto, Kazuhito</creatorcontrib><creatorcontrib>Hagino, Akihiko</creatorcontrib><creatorcontrib>Katoh, Kazuo</creatorcontrib><creatorcontrib>Obara, Yoshiaki</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative biochemistry and physiology. 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Bos taurus) were observed by reverse transcript polymerase chain reaction and immunocytochemistry. Also, changes of CA VI concentrations in milk were measured for 2 months postpartum by an enzyme-linked immunosorbent assay. CA VI gene expression was detected in the gland tissues and epithelial cells, and CA VI protein was localized in the cytoplasm of the epithelial cells. Colostrum contained the highest concentration of CA VI protein (100 ng/ml), decreasing in an exponential manner (
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| subjects | Animals Carbonic anhydrase VI Carbonic Anhydrases - analysis Carbonic Anhydrases - genetics Carbonic Anhydrases - metabolism Carbonic Anhydrases - secretion Cattle - physiology Clone Cells Colostrum Colostrum - chemistry Cow DNA, Complementary - metabolism Enzyme-Linked Immunosorbent Assay Epithelial Cells - enzymology Female Immunocytochemistry Immunohistochemistry Lactation Mammary epithelial cells Mammary Glands, Animal - cytology Mammary Glands, Animal - enzymology Milk - chemistry Neonate Osmolar Concentration Parotid Gland - enzymology Protein Subunits - analysis Protein Subunits - genetics Protein Subunits - metabolism Protein Subunits - secretion Reverse Transcriptase Polymerase Chain Reaction RT-PCR Secretion Tissue Distribution |
| title | Expression and localization of carbonic anhydrase in bovine mammary gland and secretion in milk |
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