Separation of peptides on Empore™ thin-layer chromatography sheets and blotting onto polyvinylidene difluoride membranes with subsequent gas phase sequencing

Methods for the separation of peptides on a new type of thin‐layer chromatography (TLC) sheet and blotting onto polyvinylidene difluoride (PVDF) membranes with subsequent gas phase sequencing are described. For validation, the A and B chain of insulin were chromatographed on EmporeTM TLC sheets and...

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Veröffentlicht in:	Electrophoresis 1992, Vol.13 (1), p.65-72
Hauptverfasser:	Reisinger, Peter W. M., Kleinschmidt, Traute, Welsch, Ulrich
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Chromatography, Thin Layer - instrumentation Gases Immunoblotting Membranes, Artificial Peptides - isolation & purification Polyvinyls
Online-Zugang:	Volltext
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