Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology
Summary Although colonies from Saccharomyces cerevisiae laboratory strains are smooth, those isolated from nature exhibit a structured fluffy pattern. Environmental scanning electron microscopy shows that the cells within wild fluffy colonies are connected by extracellular matrix (ECM) material. Thi...
Gespeichert in:
| Veröffentlicht in: | Molecular microbiology 2003-02, Vol.47 (3), p.745-754 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 754 |
|---|---|
| container_issue | 3 |
| container_start_page | 745 |
| container_title | Molecular microbiology |
| container_volume | 47 |
| creator | Kuthan, Martin Devaux, Frédéric Janderová, Blanka Slaninová, Iva Jacq, Claude Palková, Zdena |
| description | Summary
Although colonies from Saccharomyces cerevisiae laboratory strains are smooth, those isolated from nature exhibit a structured fluffy pattern. Environmental scanning electron microscopy shows that the cells within wild fluffy colonies are connected by extracellular matrix (ECM) material. This material contains a protein of about 200 kDa unrelated to the flocculins, proteins involved in cell–cell adhesion in liquid media. The matrix material binds to concanavalin A. Within a few passages on rich agar medium, the wild strains switch from the fluffy to the smooth colony morphology. This domestication is accompanied by loss of the ECM and by extensive changes in gene expression as detected by DNA microarrays. The expression of about 320 genes was changed in smooth colonies. The major changes comprise carbohydrate metabolism, cell wall, water channels, Ty‐transposons and subtelomeric genes, iron homeostasis, vitamin metabolism and cell cycle and polarity. The growth in fluffy colonies may represent a metabolic strategy for survival of yeast under unfavourable conditions that is switched off under felicitous laboratory conditions. |
| doi_str_mv | 10.1046/j.1365-2958.2003.03332.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72970644</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72970644</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4232-d5a86724fd6271b6dea84e143af90746138e46818f5bb56cd4e3d936ba65b06e3</originalsourceid><addsrcrecordid>eNqNkcFu1DAQhi0EokvhFZDFgVuC7bGd5MABFQqVWnEAJG6W40y2XiVxandh8_Z12BVIXOA0I803v2b0EUI5KzmT-s2u5KBVIRpVl4IxKBkAiPLwiGx-Dx6TDWsUK6AW38_Is5R2jHFgGp6SMy4UKFbBhty9DyOme-_svQ8TDT396YeOfrHO3doYxsVhog4j_vDJW6Q-0TwK42wnjx1tF5q5aZshP9EtTkjxMEdMaU2zU0ddGMK00DHE-Ta32-U5edLbIeGLUz0n3y4_fL34VFx__nh18e66cFKAKDpla10J2XdaVLzVHdpaIpdg-4ZVUnOoUeqa171qW6VdJxG6BnRrtWqZRjgnr4-5cwx3-_yjGX1yOAx2wrBPphJNxbSU_wR5voNrqDL46i9wF_Zxyk8Y3mjFuQKdofoIuRhSitibOfrRxsVwZlZ5ZmdWR2Z1ZFZ55pc8c8irL0_5-3bE7s_iyVYG3h6B7AiX_w42NzdXawcPzvWpEQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>196511536</pqid></control><display><type>article</type><title>Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology</title><source>MEDLINE</source><source>Wiley Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library All Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Kuthan, Martin ; Devaux, Frédéric ; Janderová, Blanka ; Slaninová, Iva ; Jacq, Claude ; Palková, Zdena</creator><creatorcontrib>Kuthan, Martin ; Devaux, Frédéric ; Janderová, Blanka ; Slaninová, Iva ; Jacq, Claude ; Palková, Zdena</creatorcontrib><description>Summary
Although colonies from Saccharomyces cerevisiae laboratory strains are smooth, those isolated from nature exhibit a structured fluffy pattern. Environmental scanning electron microscopy shows that the cells within wild fluffy colonies are connected by extracellular matrix (ECM) material. This material contains a protein of about 200 kDa unrelated to the flocculins, proteins involved in cell–cell adhesion in liquid media. The matrix material binds to concanavalin A. Within a few passages on rich agar medium, the wild strains switch from the fluffy to the smooth colony morphology. This domestication is accompanied by loss of the ECM and by extensive changes in gene expression as detected by DNA microarrays. The expression of about 320 genes was changed in smooth colonies. The major changes comprise carbohydrate metabolism, cell wall, water channels, Ty‐transposons and subtelomeric genes, iron homeostasis, vitamin metabolism and cell cycle and polarity. The growth in fluffy colonies may represent a metabolic strategy for survival of yeast under unfavourable conditions that is switched off under felicitous laboratory conditions.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.2003.03332.x</identifier><identifier>PMID: 12535073</identifier><language>eng</language><publisher>Oxford,UK: Blackwell Science Ltd</publisher><subject>Biological variation ; Cells ; Computational Biology ; Culture Media ; Environmental Microbiology ; Extracellular Matrix - metabolism ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Fungal ; Laboratories ; Oligonucleotide Array Sequence Analysis ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae - ultrastructure ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Scanning electron microscopy ; Transcription, Genetic ; Yeast</subject><ispartof>Molecular microbiology, 2003-02, Vol.47 (3), p.745-754</ispartof><rights>Copyright Blackwell Publishing Feb 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4232-d5a86724fd6271b6dea84e143af90746138e46818f5bb56cd4e3d936ba65b06e3</citedby><cites>FETCH-LOGICAL-c4232-d5a86724fd6271b6dea84e143af90746138e46818f5bb56cd4e3d936ba65b06e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2958.2003.03332.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2958.2003.03332.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,27923,27924,45573,45574,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12535073$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuthan, Martin</creatorcontrib><creatorcontrib>Devaux, Frédéric</creatorcontrib><creatorcontrib>Janderová, Blanka</creatorcontrib><creatorcontrib>Slaninová, Iva</creatorcontrib><creatorcontrib>Jacq, Claude</creatorcontrib><creatorcontrib>Palková, Zdena</creatorcontrib><title>Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Although colonies from Saccharomyces cerevisiae laboratory strains are smooth, those isolated from nature exhibit a structured fluffy pattern. Environmental scanning electron microscopy shows that the cells within wild fluffy colonies are connected by extracellular matrix (ECM) material. This material contains a protein of about 200 kDa unrelated to the flocculins, proteins involved in cell–cell adhesion in liquid media. The matrix material binds to concanavalin A. Within a few passages on rich agar medium, the wild strains switch from the fluffy to the smooth colony morphology. This domestication is accompanied by loss of the ECM and by extensive changes in gene expression as detected by DNA microarrays. The expression of about 320 genes was changed in smooth colonies. The major changes comprise carbohydrate metabolism, cell wall, water channels, Ty‐transposons and subtelomeric genes, iron homeostasis, vitamin metabolism and cell cycle and polarity. The growth in fluffy colonies may represent a metabolic strategy for survival of yeast under unfavourable conditions that is switched off under felicitous laboratory conditions.</description><subject>Biological variation</subject><subject>Cells</subject><subject>Computational Biology</subject><subject>Culture Media</subject><subject>Environmental Microbiology</subject><subject>Extracellular Matrix - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Laboratories</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae - ultrastructure</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Scanning electron microscopy</subject><subject>Transcription, Genetic</subject><subject>Yeast</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhi0EokvhFZDFgVuC7bGd5MABFQqVWnEAJG6W40y2XiVxandh8_Z12BVIXOA0I803v2b0EUI5KzmT-s2u5KBVIRpVl4IxKBkAiPLwiGx-Dx6TDWsUK6AW38_Is5R2jHFgGp6SMy4UKFbBhty9DyOme-_svQ8TDT396YeOfrHO3doYxsVhog4j_vDJW6Q-0TwK42wnjx1tF5q5aZshP9EtTkjxMEdMaU2zU0ddGMK00DHE-Ta32-U5edLbIeGLUz0n3y4_fL34VFx__nh18e66cFKAKDpla10J2XdaVLzVHdpaIpdg-4ZVUnOoUeqa171qW6VdJxG6BnRrtWqZRjgnr4-5cwx3-_yjGX1yOAx2wrBPphJNxbSU_wR5voNrqDL46i9wF_Zxyk8Y3mjFuQKdofoIuRhSitibOfrRxsVwZlZ5ZmdWR2Z1ZFZ55pc8c8irL0_5-3bE7s_iyVYG3h6B7AiX_w42NzdXawcPzvWpEQ</recordid><startdate>200302</startdate><enddate>200302</enddate><creator>Kuthan, Martin</creator><creator>Devaux, Frédéric</creator><creator>Janderová, Blanka</creator><creator>Slaninová, Iva</creator><creator>Jacq, Claude</creator><creator>Palková, Zdena</creator><general>Blackwell Science Ltd</general><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200302</creationdate><title>Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology</title><author>Kuthan, Martin ; Devaux, Frédéric ; Janderová, Blanka ; Slaninová, Iva ; Jacq, Claude ; Palková, Zdena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4232-d5a86724fd6271b6dea84e143af90746138e46818f5bb56cd4e3d936ba65b06e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological variation</topic><topic>Cells</topic><topic>Computational Biology</topic><topic>Culture Media</topic><topic>Environmental Microbiology</topic><topic>Extracellular Matrix - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Laboratories</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae - ultrastructure</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Scanning electron microscopy</topic><topic>Transcription, Genetic</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuthan, Martin</creatorcontrib><creatorcontrib>Devaux, Frédéric</creatorcontrib><creatorcontrib>Janderová, Blanka</creatorcontrib><creatorcontrib>Slaninová, Iva</creatorcontrib><creatorcontrib>Jacq, Claude</creatorcontrib><creatorcontrib>Palková, Zdena</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuthan, Martin</au><au>Devaux, Frédéric</au><au>Janderová, Blanka</au><au>Slaninová, Iva</au><au>Jacq, Claude</au><au>Palková, Zdena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2003-02</date><risdate>2003</risdate><volume>47</volume><issue>3</issue><spage>745</spage><epage>754</epage><pages>745-754</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Although colonies from Saccharomyces cerevisiae laboratory strains are smooth, those isolated from nature exhibit a structured fluffy pattern. Environmental scanning electron microscopy shows that the cells within wild fluffy colonies are connected by extracellular matrix (ECM) material. This material contains a protein of about 200 kDa unrelated to the flocculins, proteins involved in cell–cell adhesion in liquid media. The matrix material binds to concanavalin A. Within a few passages on rich agar medium, the wild strains switch from the fluffy to the smooth colony morphology. This domestication is accompanied by loss of the ECM and by extensive changes in gene expression as detected by DNA microarrays. The expression of about 320 genes was changed in smooth colonies. The major changes comprise carbohydrate metabolism, cell wall, water channels, Ty‐transposons and subtelomeric genes, iron homeostasis, vitamin metabolism and cell cycle and polarity. The growth in fluffy colonies may represent a metabolic strategy for survival of yeast under unfavourable conditions that is switched off under felicitous laboratory conditions.</abstract><cop>Oxford,UK</cop><pub>Blackwell Science Ltd</pub><pmid>12535073</pmid><doi>10.1046/j.1365-2958.2003.03332.x</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-382X |
| ispartof | Molecular microbiology, 2003-02, Vol.47 (3), p.745-754 |
| issn | 0950-382X 1365-2958 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72970644 |
| source | MEDLINE; Wiley Free Content; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Free Full-Text Journals in Chemistry |
| subjects | Biological variation Cells Computational Biology Culture Media Environmental Microbiology Extracellular Matrix - metabolism Gene expression Gene Expression Profiling Gene Expression Regulation, Fungal Laboratories Oligonucleotide Array Sequence Analysis Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae - ultrastructure Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Scanning electron microscopy Transcription, Genetic Yeast |
| title | Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T15%3A00%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Domestication%20of%20wild%20Saccharomyces%20cerevisiae%20is%20accompanied%20by%20changes%20in%20gene%20expression%20and%20colony%20morphology&rft.jtitle=Molecular%20microbiology&rft.au=Kuthan,%20Martin&rft.date=2003-02&rft.volume=47&rft.issue=3&rft.spage=745&rft.epage=754&rft.pages=745-754&rft.issn=0950-382X&rft.eissn=1365-2958&rft_id=info:doi/10.1046/j.1365-2958.2003.03332.x&rft_dat=%3Cproquest_cross%3E72970644%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=196511536&rft_id=info:pmid/12535073&rfr_iscdi=true |