Cu/Zn superoxide dismutase mRNA and enzyme activity, and susceptibility to lipid peroxidation, increases with aging in murine brains
To protect against reactive oxygen species, prokaryotic and eukaryotic cells have developed an antioxidant defence mechanism where O 2 − is converted to H 2O 2 by superoxide dismutase ( Sod), and in a second step, H 2O 2 is converted to H 2O by catalase ( Cat) and/or glutathione peroxidase ( Gpx). I...
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| Veröffentlicht in: | Brain research. Molecular brain research. 1992-04, Vol.13 (3), p.179-187 |
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| container_title | Brain research. Molecular brain research. |
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| creator | de Haan, Judy B. Newman, Julie D. Kola, Ismail |
| description | To protect against reactive oxygen species, prokaryotic and eukaryotic cells have developed an antioxidant defence mechanism where
O
2
− is converted to H
2O
2 by superoxide dismutase (
Sod), and in a second step, H
2O
2 is converted to H
2O by catalase (
Cat) and/or glutathione peroxidase (
Gpx). If
Sod levels are increased without a concomitant
Gpx increase, then the intermediate H
2O
2 accumulates. This intermediate could undergo the Fenton's reaction, generating hydroxyl radicals which may lead to lipid peroxidation in cells. In this study, we investigate the expression of
Sod1,
Gpx1 and susceptibility to lipid peroxidation during the aging process in mouse brains. We demonstrate that the mRNA levels and enzyme activity of
Sod1 are higher in brains from adult mice compared to neonatal mice. Furthermore, we show that a linear increase in
Sod1 mRNA and enzyme activity occurs with aging (1–100 weeks). On the contrary, we find that the mRNA and enzyme activity for
Gpx1 does not increase with aging in mouse brains. In addition, our results demonstrate that the susceptibility of murine brains to lipid peroxidation increases with aging. The data in this study are consistent with the notion that reactive oxygen species may contribute to the aging process in mammalian brains. These results are discussed in relation to the normal aging process in mammals, and to the premature aging and mental retardation in Down syndrome. |
| doi_str_mv | 10.1016/0169-328X(92)90025-7 |
| format | Article |
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O
2
− is converted to H
2O
2 by superoxide dismutase (
Sod), and in a second step, H
2O
2 is converted to H
2O by catalase (
Cat) and/or glutathione peroxidase (
Gpx). If
Sod levels are increased without a concomitant
Gpx increase, then the intermediate H
2O
2 accumulates. This intermediate could undergo the Fenton's reaction, generating hydroxyl radicals which may lead to lipid peroxidation in cells. In this study, we investigate the expression of
Sod1,
Gpx1 and susceptibility to lipid peroxidation during the aging process in mouse brains. We demonstrate that the mRNA levels and enzyme activity of
Sod1 are higher in brains from adult mice compared to neonatal mice. Furthermore, we show that a linear increase in
Sod1 mRNA and enzyme activity occurs with aging (1–100 weeks). On the contrary, we find that the mRNA and enzyme activity for
Gpx1 does not increase with aging in mouse brains. In addition, our results demonstrate that the susceptibility of murine brains to lipid peroxidation increases with aging. The data in this study are consistent with the notion that reactive oxygen species may contribute to the aging process in mammalian brains. These results are discussed in relation to the normal aging process in mammals, and to the premature aging and mental retardation in Down syndrome.</description><identifier>ISSN: 0169-328X</identifier><identifier>EISSN: 1872-6941</identifier><identifier>DOI: 10.1016/0169-328X(92)90025-7</identifier><identifier>PMID: 1593944</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Aging ; Aging - metabolism ; Alzheimer's disease ; Animals ; Animals, Newborn - metabolism ; Antioxidant enzyme ; Biological and medical sciences ; Brain - enzymology ; Catalase ; Catalase - metabolism ; Chromosome aberrations ; Down syndrome ; Enzyme Induction ; Free radical ; Free Radicals ; Glutathione peroxidase ; Glutathione Peroxidase - metabolism ; Hydrogen Peroxide - metabolism ; Lipid Peroxidation ; Medical genetics ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Oxygen - metabolism ; Parkinson's disease ; RNA, Messenger - biosynthesis ; Superoxide Dismutase - metabolism</subject><ispartof>Brain research. Molecular brain research., 1992-04, Vol.13 (3), p.179-187</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-f098700dc17d0505aee5d0e4865a17498d21885861f279a9668b9ecbd1e312913</citedby><cites>FETCH-LOGICAL-c332t-f098700dc17d0505aee5d0e4865a17498d21885861f279a9668b9ecbd1e312913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5168625$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1593944$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Haan, Judy B.</creatorcontrib><creatorcontrib>Newman, Julie D.</creatorcontrib><creatorcontrib>Kola, Ismail</creatorcontrib><title>Cu/Zn superoxide dismutase mRNA and enzyme activity, and susceptibility to lipid peroxidation, increases with aging in murine brains</title><title>Brain research. Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>To protect against reactive oxygen species, prokaryotic and eukaryotic cells have developed an antioxidant defence mechanism where
O
2
− is converted to H
2O
2 by superoxide dismutase (
Sod), and in a second step, H
2O
2 is converted to H
2O by catalase (
Cat) and/or glutathione peroxidase (
Gpx). If
Sod levels are increased without a concomitant
Gpx increase, then the intermediate H
2O
2 accumulates. This intermediate could undergo the Fenton's reaction, generating hydroxyl radicals which may lead to lipid peroxidation in cells. In this study, we investigate the expression of
Sod1,
Gpx1 and susceptibility to lipid peroxidation during the aging process in mouse brains. We demonstrate that the mRNA levels and enzyme activity of
Sod1 are higher in brains from adult mice compared to neonatal mice. Furthermore, we show that a linear increase in
Sod1 mRNA and enzyme activity occurs with aging (1–100 weeks). On the contrary, we find that the mRNA and enzyme activity for
Gpx1 does not increase with aging in mouse brains. In addition, our results demonstrate that the susceptibility of murine brains to lipid peroxidation increases with aging. The data in this study are consistent with the notion that reactive oxygen species may contribute to the aging process in mammalian brains. These results are discussed in relation to the normal aging process in mammals, and to the premature aging and mental retardation in Down syndrome.</description><subject>Aging</subject><subject>Aging - metabolism</subject><subject>Alzheimer's disease</subject><subject>Animals</subject><subject>Animals, Newborn - metabolism</subject><subject>Antioxidant enzyme</subject><subject>Biological and medical sciences</subject><subject>Brain - enzymology</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Chromosome aberrations</subject><subject>Down syndrome</subject><subject>Enzyme Induction</subject><subject>Free radical</subject><subject>Free Radicals</subject><subject>Glutathione peroxidase</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Lipid Peroxidation</subject><subject>Medical genetics</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Oxygen - metabolism</subject><subject>Parkinson's disease</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Superoxide Dismutase - metabolism</subject><issn>0169-328X</issn><issn>1872-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rFDEUxYModa1-A4U8iCh0bJKZySQvQlnqHygKoiC-hExyt16ZyYxJpnZ99oOb7S71TR9C4NzfOdzkEPKYs5eccXlajq5qob481-KFZky0VXeHrLjqRCV1w--S1S1ynzxI6TtjjCvOj8gRb3Wtm2ZFfq-X06-BpmWGOF2jB-oxjUu2Cej48f0ZtcFTCL-2I1DrMl5h3p7ciGlJDuaMPQ5Fo3miA87o6SHIZpzCCcXgIpSwRH9i_kbtJYbLItJxiRiA9tFiSA_JvY0dEjw63Mfk8-vzT-u31cWHN-_WZxeVq2uRqw3TqmPMO9551rLWArSeQaNka3nXaOUFV6pVkm9Ep62WUvUaXO851FxoXh-TZ_vcOU4_FkjZjFgeMQw2wLQk04ni4Ur_F-SykUrUO7DZgy5OKUXYmDniaOPWcGZ2LZldBWZXgdHC3LRkumJ7cshf-hH8X9O-ljJ_epjb5OywiTY4TLdYy6WSoi3Yqz0G5dOuEKJJDiE48BjBZeMn_PcefwBVu69Y</recordid><startdate>199204</startdate><enddate>199204</enddate><creator>de Haan, Judy B.</creator><creator>Newman, Julie D.</creator><creator>Kola, Ismail</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199204</creationdate><title>Cu/Zn superoxide dismutase mRNA and enzyme activity, and susceptibility to lipid peroxidation, increases with aging in murine brains</title><author>de Haan, Judy B. ; Newman, Julie D. ; Kola, Ismail</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-f098700dc17d0505aee5d0e4865a17498d21885861f279a9668b9ecbd1e312913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Aging</topic><topic>Aging - metabolism</topic><topic>Alzheimer's disease</topic><topic>Animals</topic><topic>Animals, Newborn - metabolism</topic><topic>Antioxidant enzyme</topic><topic>Biological and medical sciences</topic><topic>Brain - enzymology</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>Chromosome aberrations</topic><topic>Down syndrome</topic><topic>Enzyme Induction</topic><topic>Free radical</topic><topic>Free Radicals</topic><topic>Glutathione peroxidase</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Lipid Peroxidation</topic><topic>Medical genetics</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>Oxygen - metabolism</topic><topic>Parkinson's disease</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Superoxide Dismutase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Haan, Judy B.</creatorcontrib><creatorcontrib>Newman, Julie D.</creatorcontrib><creatorcontrib>Kola, Ismail</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Molecular brain research.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Haan, Judy B.</au><au>Newman, Julie D.</au><au>Kola, Ismail</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cu/Zn superoxide dismutase mRNA and enzyme activity, and susceptibility to lipid peroxidation, increases with aging in murine brains</atitle><jtitle>Brain research. Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>1992-04</date><risdate>1992</risdate><volume>13</volume><issue>3</issue><spage>179</spage><epage>187</epage><pages>179-187</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>To protect against reactive oxygen species, prokaryotic and eukaryotic cells have developed an antioxidant defence mechanism where
O
2
− is converted to H
2O
2 by superoxide dismutase (
Sod), and in a second step, H
2O
2 is converted to H
2O by catalase (
Cat) and/or glutathione peroxidase (
Gpx). If
Sod levels are increased without a concomitant
Gpx increase, then the intermediate H
2O
2 accumulates. This intermediate could undergo the Fenton's reaction, generating hydroxyl radicals which may lead to lipid peroxidation in cells. In this study, we investigate the expression of
Sod1,
Gpx1 and susceptibility to lipid peroxidation during the aging process in mouse brains. We demonstrate that the mRNA levels and enzyme activity of
Sod1 are higher in brains from adult mice compared to neonatal mice. Furthermore, we show that a linear increase in
Sod1 mRNA and enzyme activity occurs with aging (1–100 weeks). On the contrary, we find that the mRNA and enzyme activity for
Gpx1 does not increase with aging in mouse brains. In addition, our results demonstrate that the susceptibility of murine brains to lipid peroxidation increases with aging. The data in this study are consistent with the notion that reactive oxygen species may contribute to the aging process in mammalian brains. These results are discussed in relation to the normal aging process in mammals, and to the premature aging and mental retardation in Down syndrome.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1593944</pmid><doi>10.1016/0169-328X(92)90025-7</doi><tpages>9</tpages></addata></record> |
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| ispartof | Brain research. Molecular brain research., 1992-04, Vol.13 (3), p.179-187 |
| issn | 0169-328X 1872-6941 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72966189 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Aging Aging - metabolism Alzheimer's disease Animals Animals, Newborn - metabolism Antioxidant enzyme Biological and medical sciences Brain - enzymology Catalase Catalase - metabolism Chromosome aberrations Down syndrome Enzyme Induction Free radical Free Radicals Glutathione peroxidase Glutathione Peroxidase - metabolism Hydrogen Peroxide - metabolism Lipid Peroxidation Medical genetics Medical sciences Mice Mice, Inbred C57BL Mice, Inbred CBA Oxygen - metabolism Parkinson's disease RNA, Messenger - biosynthesis Superoxide Dismutase - metabolism |
| title | Cu/Zn superoxide dismutase mRNA and enzyme activity, and susceptibility to lipid peroxidation, increases with aging in murine brains |
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