Thermodynamics of ribonuclease T1 denaturation

Differential scanning calorimetry has been used to investigate the thermodynamics of denaturation of ribonuclease T1 as a function of pH over the pH range 2-10, and as a function of NaCl and MgCl2 concentration. At pH 7 in 30 mM PIPES buffer, the thermodynamic parameters are as follows: melting temp...

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Veröffentlicht in:	Biochemistry (Easton) 1992-05, Vol.31 (20), p.4876-4882
Hauptverfasser:	Hu, Cui Qing, Sturtevant, Julian M, Thomson, James A, Erickson, Rick E, Pace, C. Nick
Format:	Artikel
Sprache:	eng
Schlagworte:	Calorimetry, Differential Scanning Enzyme Stability Hydrogen-Ion Concentration Protein Conformation Protein Denaturation Ribonuclease T1 - chemistry Spectrometry, Fluorescence Thermodynamics
Online-Zugang:	Volltext
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