Specific MR imaging of human lymphocytes by monoclonal antibody-guided dextran-magnetite particles
Human lymphocytes were labeled with biotinylated anti‐lymphocyte‐directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran‐magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2...
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| Veröffentlicht in: | Magnetic resonance in medicine 1992-05, Vol.25 (1), p.148-157 |
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| container_title | Magnetic resonance in medicine |
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| creator | Bulte, Jeff W. M. Hoekstra, Yke Kamman, Richard L. Magin, Richard L. Webb, Andrew G. Briggs, Richard W. Gwan Go, K. Hulstaert, Caesar E. Miltenyi, Stefan Hauw The, T. De Leij, Lou |
| description | Human lymphocytes were labeled with biotinylated anti‐lymphocyte‐directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran‐magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R2. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging. |
| doi_str_mv | 10.1002/mrm.1910250115 |
| format | Article |
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M. ; Hoekstra, Yke ; Kamman, Richard L. ; Magin, Richard L. ; Webb, Andrew G. ; Briggs, Richard W. ; Gwan Go, K. ; Hulstaert, Caesar E. ; Miltenyi, Stefan ; Hauw The, T. ; De Leij, Lou</creator><creatorcontrib>Bulte, Jeff W. M. ; Hoekstra, Yke ; Kamman, Richard L. ; Magin, Richard L. ; Webb, Andrew G. ; Briggs, Richard W. ; Gwan Go, K. ; Hulstaert, Caesar E. ; Miltenyi, Stefan ; Hauw The, T. ; De Leij, Lou</creatorcontrib><description>Human lymphocytes were labeled with biotinylated anti‐lymphocyte‐directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran‐magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R2. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.</description><identifier>ISSN: 0740-3194</identifier><identifier>EISSN: 1522-2594</identifier><identifier>DOI: 10.1002/mrm.1910250115</identifier><identifier>PMID: 1375703</identifier><language>eng</language><publisher>Baltimore: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Antibodies, Monoclonal ; Bacterial Proteins ; Contrast Media ; Dextrans ; Ferrosoferric Oxide ; Humans ; In Vitro Techniques ; Iron ; Lymphocytes - pathology ; Magnetic Resonance Imaging - methods ; Oxides ; Streptavidin</subject><ispartof>Magnetic resonance in medicine, 1992-05, Vol.25 (1), p.148-157</ispartof><rights>Copyright © 1992 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4445-822e3c56c7278b4be7d3cc2db9eaa6a92f56106ab6b1f967b5a67a64cfc09c6b3</citedby><cites>FETCH-LOGICAL-c4445-822e3c56c7278b4be7d3cc2db9eaa6a92f56106ab6b1f967b5a67a64cfc09c6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrm.1910250115$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrm.1910250115$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1375703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bulte, Jeff W. 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This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R2. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.</description><subject>Antibodies, Monoclonal</subject><subject>Bacterial Proteins</subject><subject>Contrast Media</subject><subject>Dextrans</subject><subject>Ferrosoferric Oxide</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Iron</subject><subject>Lymphocytes - pathology</subject><subject>Magnetic Resonance Imaging - methods</subject><subject>Oxides</subject><subject>Streptavidin</subject><issn>0740-3194</issn><issn>1522-2594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAURa2qFR1ot-yQvOouU9vxB16iEQwVM61EqVhatvMyuCRxsBOV_PumCirqqqu3uPce6R2ETilZU0LY5za1a6opYYJQKt6gFRWMFUxo_hatiOKkKKnm79Fxzj8JIVorfoSOaKmEIuUKue89-FAHj_e3OLT2ELoDjjV-GFvb4WZq-4fopwEydhNuYxd9EzvbYNsNwcVqKg5jqKDCFTwPyXbFTOhgCAPg3qYh-AbyB_Sutk2Gjy_3BP24urzbXBe7b9svm4td4TnnojhnDEovpFdMnTvuQFWl96xyGqyVVrNaSEqkddLRWkvlhJXKSu5rT7SXrjxBnxZun-LTCHkwbcgemsZ2EMdsFNOSSyrm4nop-hRzTlCbPs2vp8lQYv5INbNU8yp1Hpy9kEfXQvVaXyzOuV7yX6GB6T80s7_d_8Mulm3IAzz_3dr0aKSa-eb-69ZsyO7uRpF7sy1_AxT5lEo</recordid><startdate>199205</startdate><enddate>199205</enddate><creator>Bulte, Jeff W. 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M.</creatorcontrib><creatorcontrib>Hoekstra, Yke</creatorcontrib><creatorcontrib>Kamman, Richard L.</creatorcontrib><creatorcontrib>Magin, Richard L.</creatorcontrib><creatorcontrib>Webb, Andrew G.</creatorcontrib><creatorcontrib>Briggs, Richard W.</creatorcontrib><creatorcontrib>Gwan Go, K.</creatorcontrib><creatorcontrib>Hulstaert, Caesar E.</creatorcontrib><creatorcontrib>Miltenyi, Stefan</creatorcontrib><creatorcontrib>Hauw The, T.</creatorcontrib><creatorcontrib>De Leij, Lou</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Magnetic resonance in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bulte, Jeff W. M.</au><au>Hoekstra, Yke</au><au>Kamman, Richard L.</au><au>Magin, Richard L.</au><au>Webb, Andrew G.</au><au>Briggs, Richard W.</au><au>Gwan Go, K.</au><au>Hulstaert, Caesar E.</au><au>Miltenyi, Stefan</au><au>Hauw The, T.</au><au>De Leij, Lou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific MR imaging of human lymphocytes by monoclonal antibody-guided dextran-magnetite particles</atitle><jtitle>Magnetic resonance in medicine</jtitle><addtitle>Magn. Reson. Med</addtitle><date>1992-05</date><risdate>1992</risdate><volume>25</volume><issue>1</issue><spage>148</spage><epage>157</epage><pages>148-157</pages><issn>0740-3194</issn><eissn>1522-2594</eissn><abstract>Human lymphocytes were labeled with biotinylated anti‐lymphocyte‐directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran‐magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R2. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.</abstract><cop>Baltimore</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>1375703</pmid><doi>10.1002/mrm.1910250115</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0740-3194 |
| ispartof | Magnetic resonance in medicine, 1992-05, Vol.25 (1), p.148-157 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Antibodies, Monoclonal Bacterial Proteins Contrast Media Dextrans Ferrosoferric Oxide Humans In Vitro Techniques Iron Lymphocytes - pathology Magnetic Resonance Imaging - methods Oxides Streptavidin |
| title | Specific MR imaging of human lymphocytes by monoclonal antibody-guided dextran-magnetite particles |
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