Alteration of Lipids, G Proteins, and PKC in Cell Membranes of Elderly Hypertensives
ABSTRACT—In this study, we quantified the levels of lipids and signaling proteins in erythrocyte membranes from elderly normotensive and hypertensive subjects. In hypertensive subjects, the cholesterol/phospholipid ratio increased significantly in erythrocyte membranes, owing to the reduction of pho...
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| Veröffentlicht in: | Hypertension (Dallas, Tex. 1979) Tex. 1979), 2003-01, Vol.41 (1), p.176-182 |
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| container_title | Hypertension (Dallas, Tex. 1979) |
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| creator | Escribá, Pablo V Sánchez-Dominguez, José M Alemany, Regina Perona, Javier S Ruiz-Gutiérrez, Valentina |
| description | ABSTRACT—In this study, we quantified the levels of lipids and signaling proteins in erythrocyte membranes from elderly normotensive and hypertensive subjects. In hypertensive subjects, the cholesterol/phospholipid ratio increased significantly in erythrocyte membranes, owing to the reduction of phospholipid levels concomitant with a rise in the levels of cholesterol. In addition, differences were also found in the amount of fatty acids in both phospholipid and cholesterol esters. Erythrocyte membranes from hypertensive subjects contained higher levels of monounsaturated and lower levels of polyunsaturated fatty acids. On the other hand, signaling proteins such as G proteins and protein kinase C have been implicated in the control of blood pressure. Previous studies have shown that the cellular localization and the activity of these proteins are modulated by the type and the abundance of membrane lipids. For this reason, we assessed the levels of these signaling molecules in the membrane. We found that the levels of membrane-associated (active/preactive) G proteins (Gαi, Gαo, and Gβ) and protein kinase C were significantly reduced in hypertensive subjects. We believe that these alterations could be related to the etiopathology of hypertension in elderly subjects or alternatively may correspond to adaptive compensatory mechanisms. |
| doi_str_mv | 10.1161/01.HYP.0000047647.72162.A8 |
| format | Article |
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In hypertensive subjects, the cholesterol/phospholipid ratio increased significantly in erythrocyte membranes, owing to the reduction of phospholipid levels concomitant with a rise in the levels of cholesterol. In addition, differences were also found in the amount of fatty acids in both phospholipid and cholesterol esters. Erythrocyte membranes from hypertensive subjects contained higher levels of monounsaturated and lower levels of polyunsaturated fatty acids. On the other hand, signaling proteins such as G proteins and protein kinase C have been implicated in the control of blood pressure. Previous studies have shown that the cellular localization and the activity of these proteins are modulated by the type and the abundance of membrane lipids. For this reason, we assessed the levels of these signaling molecules in the membrane. We found that the levels of membrane-associated (active/preactive) G proteins (Gαi, Gαo, and Gβ) and protein kinase C were significantly reduced in hypertensive subjects. We believe that these alterations could be related to the etiopathology of hypertension in elderly subjects or alternatively may correspond to adaptive compensatory mechanisms.</description><identifier>ISSN: 0194-911X</identifier><identifier>EISSN: 1524-4563</identifier><identifier>DOI: 10.1161/01.HYP.0000047647.72162.A8</identifier><identifier>PMID: 12511549</identifier><identifier>CODEN: HPRTDN</identifier><language>eng</language><publisher>Philadelphia, PA: American Heart Association, Inc</publisher><subject>Aged ; Aged, 80 and over ; Arterial hypertension. Arterial hypotension ; Biological and medical sciences ; Blood and lymphatic vessels ; Cardiology. Vascular system ; Clinical manifestations. Epidemiology. Investigative techniques. Etiology ; Erythrocyte Membrane - chemistry ; Erythrocyte Membrane - enzymology ; Female ; Heterotrimeric GTP-Binding Proteins - analysis ; Humans ; Hypertension - blood ; Hypertension - enzymology ; Hypertension - metabolism ; Isoenzymes - analysis ; Lipoproteins - blood ; Male ; Medical sciences ; Membrane Lipids - analysis ; Protein Kinase C - analysis ; Protein Kinase C-alpha ; Protein Subunits</subject><ispartof>Hypertension (Dallas, Tex. 1979), 2003-01, Vol.41 (1), p.176-182</ispartof><rights>2003 American Heart Association, Inc.</rights><rights>2003 INIST-CNRS</rights><rights>Copyright American Heart Association, Inc. 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In hypertensive subjects, the cholesterol/phospholipid ratio increased significantly in erythrocyte membranes, owing to the reduction of phospholipid levels concomitant with a rise in the levels of cholesterol. In addition, differences were also found in the amount of fatty acids in both phospholipid and cholesterol esters. Erythrocyte membranes from hypertensive subjects contained higher levels of monounsaturated and lower levels of polyunsaturated fatty acids. On the other hand, signaling proteins such as G proteins and protein kinase C have been implicated in the control of blood pressure. Previous studies have shown that the cellular localization and the activity of these proteins are modulated by the type and the abundance of membrane lipids. For this reason, we assessed the levels of these signaling molecules in the membrane. We found that the levels of membrane-associated (active/preactive) G proteins (Gαi, Gαo, and Gβ) and protein kinase C were significantly reduced in hypertensive subjects. We believe that these alterations could be related to the etiopathology of hypertension in elderly subjects or alternatively may correspond to adaptive compensatory mechanisms.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Arterial hypertension. Arterial hypotension</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Cardiology. Vascular system</subject><subject>Clinical manifestations. Epidemiology. Investigative techniques. Etiology</subject><subject>Erythrocyte Membrane - chemistry</subject><subject>Erythrocyte Membrane - enzymology</subject><subject>Female</subject><subject>Heterotrimeric GTP-Binding Proteins - analysis</subject><subject>Humans</subject><subject>Hypertension - blood</subject><subject>Hypertension - enzymology</subject><subject>Hypertension - metabolism</subject><subject>Isoenzymes - analysis</subject><subject>Lipoproteins - blood</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Lipids - analysis</subject><subject>Protein Kinase C - analysis</subject><subject>Protein Kinase C-alpha</subject><subject>Protein Subunits</subject><issn>0194-911X</issn><issn>1524-4563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV-LEzEUxYMobl39ChIW9MkZc_NnkvhWyroVK_ZhBX0KmZkMmzWdqcmMS7-9mbZQMBByE34n9-QEoRsgJUAFHwmU61_bksyDy4rLUlKoaLlUz9ACBOUFFxV7jhYENC80wM8r9CqlR0KAcy5foiugAkBwvUD3yzC6aEc_9Hjo8MbvfZs-4Du8jcPofJ9r27d4-3WFfY9XLgT8ze3qaHuXZsFtaF0MB7w-7F0cXZ_8X5deoxedDcm9Oa_X6Mfn2_vVuth8v_uyWm6KRshKF1oxpbmtW0VstqNqYEI1su7alilrOas621EKsuaM58JKLlU-raxy-YHArtH70737OPyZXBrNzqcme8zuhikZSbWArM_gzX_g4zDFPnszlAgqJegZ-nSCmjikFF1n9tHvbDwYIGYO3hAwOXhzCd4cgzdLlcVvzx2meufai_ScdAbenQGbGhu6nGDj04XjvJLi-CZ-4p6G-WfS7zA9uWgenA3jw7E1p5UqKCGMQN4VeVLN_gFcyZip</recordid><startdate>200301</startdate><enddate>200301</enddate><creator>Escribá, Pablo V</creator><creator>Sánchez-Dominguez, José M</creator><creator>Alemany, Regina</creator><creator>Perona, Javier S</creator><creator>Ruiz-Gutiérrez, Valentina</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>200301</creationdate><title>Alteration of Lipids, G Proteins, and PKC in Cell Membranes of Elderly Hypertensives</title><author>Escribá, Pablo V ; Sánchez-Dominguez, José M ; Alemany, Regina ; Perona, Javier S ; Ruiz-Gutiérrez, Valentina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5769-983894abd80a1158b1358c7bfdd38aa436faf2217b434f22a74784366a8e56313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Arterial hypertension. Arterial hypotension</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Cardiology. Vascular system</topic><topic>Clinical manifestations. Epidemiology. Investigative techniques. Etiology</topic><topic>Erythrocyte Membrane - chemistry</topic><topic>Erythrocyte Membrane - enzymology</topic><topic>Female</topic><topic>Heterotrimeric GTP-Binding Proteins - analysis</topic><topic>Humans</topic><topic>Hypertension - blood</topic><topic>Hypertension - enzymology</topic><topic>Hypertension - metabolism</topic><topic>Isoenzymes - analysis</topic><topic>Lipoproteins - blood</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Lipids - analysis</topic><topic>Protein Kinase C - analysis</topic><topic>Protein Kinase C-alpha</topic><topic>Protein Subunits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Escribá, Pablo V</creatorcontrib><creatorcontrib>Sánchez-Dominguez, José M</creatorcontrib><creatorcontrib>Alemany, Regina</creatorcontrib><creatorcontrib>Perona, Javier S</creatorcontrib><creatorcontrib>Ruiz-Gutiérrez, Valentina</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Hypertension (Dallas, Tex. 1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Escribá, Pablo V</au><au>Sánchez-Dominguez, José M</au><au>Alemany, Regina</au><au>Perona, Javier S</au><au>Ruiz-Gutiérrez, Valentina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alteration of Lipids, G Proteins, and PKC in Cell Membranes of Elderly Hypertensives</atitle><jtitle>Hypertension (Dallas, Tex. 1979)</jtitle><addtitle>Hypertension</addtitle><date>2003-01</date><risdate>2003</risdate><volume>41</volume><issue>1</issue><spage>176</spage><epage>182</epage><pages>176-182</pages><issn>0194-911X</issn><eissn>1524-4563</eissn><coden>HPRTDN</coden><abstract>ABSTRACT—In this study, we quantified the levels of lipids and signaling proteins in erythrocyte membranes from elderly normotensive and hypertensive subjects. In hypertensive subjects, the cholesterol/phospholipid ratio increased significantly in erythrocyte membranes, owing to the reduction of phospholipid levels concomitant with a rise in the levels of cholesterol. In addition, differences were also found in the amount of fatty acids in both phospholipid and cholesterol esters. Erythrocyte membranes from hypertensive subjects contained higher levels of monounsaturated and lower levels of polyunsaturated fatty acids. On the other hand, signaling proteins such as G proteins and protein kinase C have been implicated in the control of blood pressure. Previous studies have shown that the cellular localization and the activity of these proteins are modulated by the type and the abundance of membrane lipids. For this reason, we assessed the levels of these signaling molecules in the membrane. We found that the levels of membrane-associated (active/preactive) G proteins (Gαi, Gαo, and Gβ) and protein kinase C were significantly reduced in hypertensive subjects. We believe that these alterations could be related to the etiopathology of hypertension in elderly subjects or alternatively may correspond to adaptive compensatory mechanisms.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>12511549</pmid><doi>10.1161/01.HYP.0000047647.72162.A8</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Aged, 80 and over Arterial hypertension. Arterial hypotension Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Clinical manifestations. Epidemiology. Investigative techniques. Etiology Erythrocyte Membrane - chemistry Erythrocyte Membrane - enzymology Female Heterotrimeric GTP-Binding Proteins - analysis Humans Hypertension - blood Hypertension - enzymology Hypertension - metabolism Isoenzymes - analysis Lipoproteins - blood Male Medical sciences Membrane Lipids - analysis Protein Kinase C - analysis Protein Kinase C-alpha Protein Subunits |
| title | Alteration of Lipids, G Proteins, and PKC in Cell Membranes of Elderly Hypertensives |
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