Co-translational incorporation of trans-4-hydroxyproline into recombinant proteins in bacteria

Trans-4-hydroxyproline (Hyp) in eukaryotic proteins arises from post-translational modification of proline residues. Because the modification enzyme is not present in prokaryotes, no natural means exists to incorporate Hyp into proteins synthesized in Escherichia coli. We show here that under approp...

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Veröffentlicht in:	The Journal of biological chemistry 2003-01, Vol.278 (1), p.645-650
Hauptverfasser:	Buechter, Douglas D, Paolella, David N, Leslie, Bridget S, Brown, Melissa S, Mehos, Karen A, Gruskin, Elliott A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acyl-tRNA Synthetases - genetics Amino Acyl-tRNA Synthetases - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Circular Dichroism Codon Collagen Type I - genetics Collagen Type I - metabolism Escherichia coli - genetics Escherichia coli - metabolism Genes, Bacterial Glutathione Transferase - genetics Glutathione Transferase - metabolism Humans Hydroxyproline - chemistry Hydroxyproline - genetics Hydroxyproline - metabolism Molecular Sequence Data Proline - metabolism Protein Biosynthesis Recombinant Proteins - genetics Recombinant Proteins - metabolism
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