Chromosomal abnormalities in embryos derived from testicular sperm extraction
To compare the rate of chromosome abnormalities in embryos obtained from karyotypically normal patients with nonobstructive azoospermia undergoing testicular sperm extraction (TESE) to those from patients undergoing intracytoplasmic sperm injection (ICSI) with ejaculated sperm. Retrospective analysi...
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| Veröffentlicht in: | Fertility and sterility 2003, Vol.79 (1), p.30-38 |
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| creator | Silber, Sherman Escudero, Tomas Lenahan, Kathleen Abdelhadi, Iman Kilani, Zaid Munné, Santiago |
| description | To compare the rate of chromosome abnormalities in embryos obtained from karyotypically normal patients with nonobstructive azoospermia undergoing testicular sperm extraction (TESE) to those from patients undergoing intracytoplasmic sperm injection (ICSI) with ejaculated sperm.
Retrospective analysis.
IVF centers.
Male partners had either nonobstructive zoospermia or oligospermia.
Preimplantation genetic diagnosis. Chromosome enumeration was performed by fluorescence in situ hybridization (FISH). Embryos classified as abnormal were reanalyzed to study mosaicism.
Chromosome abnormalities in embryos.
Embryos from ICSI cycles with ejaculated sperm (group 1) were 41.8% normal, 26.2% aneuploid, and 26.5% mosaic. In contrast, the embryos from ICSI cycles with TESE for nonobstructive azoospermia (group 2) were 22% normal, 17% aneuploid, and 53% mosaic. The difference in mosaicism rate between the two groups of embryos was highly significant.
The present study results indicate a high incidence of mosaicism in embryos derived from TESE in men with a severe deficit in spermatogenesis. Sperm derived from TESE for nonobstructive azoospermia may have a higher rate of compromised or immature centrosome structures leading to mosaicism in the embryo. |
| doi_str_mv | 10.1016/S0015-0282(02)04407-2 |
| format | Article |
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Retrospective analysis.
IVF centers.
Male partners had either nonobstructive zoospermia or oligospermia.
Preimplantation genetic diagnosis. Chromosome enumeration was performed by fluorescence in situ hybridization (FISH). Embryos classified as abnormal were reanalyzed to study mosaicism.
Chromosome abnormalities in embryos.
Embryos from ICSI cycles with ejaculated sperm (group 1) were 41.8% normal, 26.2% aneuploid, and 26.5% mosaic. In contrast, the embryos from ICSI cycles with TESE for nonobstructive azoospermia (group 2) were 22% normal, 17% aneuploid, and 53% mosaic. The difference in mosaicism rate between the two groups of embryos was highly significant.
The present study results indicate a high incidence of mosaicism in embryos derived from TESE in men with a severe deficit in spermatogenesis. Sperm derived from TESE for nonobstructive azoospermia may have a higher rate of compromised or immature centrosome structures leading to mosaicism in the embryo.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(02)04407-2</identifier><identifier>PMID: 12524060</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Aneuploidy ; Biological and medical sciences ; chaotic ; Chromosome Aberrations ; Humans ; In Situ Hybridization, Fluorescence ; Male ; Medical genetics ; Medical sciences ; Mosaicism ; Oligospermia - therapy ; PGD ; Preimplantation Diagnosis ; preimplantation genetic diagnosis ; Retrospective Studies ; Sperm Injections, Intracytoplasmic ; Spermatozoa ; Testis - cytology ; Tissue and Organ Harvesting - methods</subject><ispartof>Fertility and sterility, 2003, Vol.79 (1), p.30-38</ispartof><rights>2003 American Society for Reproductive Medicine</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-b18e6074f35c6073795db41ea2e63c2e9ee5ba8ed04fe05da51d4341c760a2233</citedby><cites>FETCH-LOGICAL-c490t-b18e6074f35c6073795db41ea2e63c2e9ee5ba8ed04fe05da51d4341c760a2233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0015-0282(02)04407-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4022,27921,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14474509$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12524060$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silber, Sherman</creatorcontrib><creatorcontrib>Escudero, Tomas</creatorcontrib><creatorcontrib>Lenahan, Kathleen</creatorcontrib><creatorcontrib>Abdelhadi, Iman</creatorcontrib><creatorcontrib>Kilani, Zaid</creatorcontrib><creatorcontrib>Munné, Santiago</creatorcontrib><title>Chromosomal abnormalities in embryos derived from testicular sperm extraction</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>To compare the rate of chromosome abnormalities in embryos obtained from karyotypically normal patients with nonobstructive azoospermia undergoing testicular sperm extraction (TESE) to those from patients undergoing intracytoplasmic sperm injection (ICSI) with ejaculated sperm.
Retrospective analysis.
IVF centers.
Male partners had either nonobstructive zoospermia or oligospermia.
Preimplantation genetic diagnosis. Chromosome enumeration was performed by fluorescence in situ hybridization (FISH). Embryos classified as abnormal were reanalyzed to study mosaicism.
Chromosome abnormalities in embryos.
Embryos from ICSI cycles with ejaculated sperm (group 1) were 41.8% normal, 26.2% aneuploid, and 26.5% mosaic. In contrast, the embryos from ICSI cycles with TESE for nonobstructive azoospermia (group 2) were 22% normal, 17% aneuploid, and 53% mosaic. The difference in mosaicism rate between the two groups of embryos was highly significant.
The present study results indicate a high incidence of mosaicism in embryos derived from TESE in men with a severe deficit in spermatogenesis. Sperm derived from TESE for nonobstructive azoospermia may have a higher rate of compromised or immature centrosome structures leading to mosaicism in the embryo.</description><subject>Aneuploidy</subject><subject>Biological and medical sciences</subject><subject>chaotic</subject><subject>Chromosome Aberrations</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Male</subject><subject>Medical genetics</subject><subject>Medical sciences</subject><subject>Mosaicism</subject><subject>Oligospermia - therapy</subject><subject>PGD</subject><subject>Preimplantation Diagnosis</subject><subject>preimplantation genetic diagnosis</subject><subject>Retrospective Studies</subject><subject>Sperm Injections, Intracytoplasmic</subject><subject>Spermatozoa</subject><subject>Testis - cytology</subject><subject>Tissue and Organ Harvesting - methods</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PwzAMhiMEYmPwE0C9gOBQcNKkXU8ITXxJQxyAc5Qmrghqm5F0E_v3ZB9iRy62D4_tVw8hpxSuKdD85g2AihTYmF0CuwLOoUjZHhlSIfJU5CLbJ8M_ZECOQvgCgJwW7JAMKBOMQw5D8jL59K51wbWqSVTVOR8H21sMie0SbCu_dCEx6O0CTVJHNukx9FbPG-WTMEPfJvjTe6V767pjclCrJuDJto_Ix8P9--Qpnb4-Pk_upqnmJfRpRceYQ8HrTOjYs6IUpuIUFcM80wxLRFGpMRrgNYIwSlDDM051kYNiLMtG5GJzd-bd9zzmka0NGptGdejmQRas5JEVERQbUHsXgsdazrxtlV9KCnLlUa49ypWkWOTao2Rx72z7YF61aHZbW3ERON8CKmjV1F512oYdx3nBBZSRu91wGHUsLHoZtMVOo7EedS-Ns_9E-QV_kI-w</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Silber, Sherman</creator><creator>Escudero, Tomas</creator><creator>Lenahan, Kathleen</creator><creator>Abdelhadi, Iman</creator><creator>Kilani, Zaid</creator><creator>Munné, Santiago</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Chromosomal abnormalities in embryos derived from testicular sperm extraction</title><author>Silber, Sherman ; Escudero, Tomas ; Lenahan, Kathleen ; Abdelhadi, Iman ; Kilani, Zaid ; Munné, Santiago</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-b18e6074f35c6073795db41ea2e63c2e9ee5ba8ed04fe05da51d4341c760a2233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aneuploidy</topic><topic>Biological and medical sciences</topic><topic>chaotic</topic><topic>Chromosome Aberrations</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Male</topic><topic>Medical genetics</topic><topic>Medical sciences</topic><topic>Mosaicism</topic><topic>Oligospermia - therapy</topic><topic>PGD</topic><topic>Preimplantation Diagnosis</topic><topic>preimplantation genetic diagnosis</topic><topic>Retrospective Studies</topic><topic>Sperm Injections, Intracytoplasmic</topic><topic>Spermatozoa</topic><topic>Testis - cytology</topic><topic>Tissue and Organ Harvesting - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silber, Sherman</creatorcontrib><creatorcontrib>Escudero, Tomas</creatorcontrib><creatorcontrib>Lenahan, Kathleen</creatorcontrib><creatorcontrib>Abdelhadi, Iman</creatorcontrib><creatorcontrib>Kilani, Zaid</creatorcontrib><creatorcontrib>Munné, Santiago</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silber, Sherman</au><au>Escudero, Tomas</au><au>Lenahan, Kathleen</au><au>Abdelhadi, Iman</au><au>Kilani, Zaid</au><au>Munné, Santiago</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromosomal abnormalities in embryos derived from testicular sperm extraction</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2003</date><risdate>2003</risdate><volume>79</volume><issue>1</issue><spage>30</spage><epage>38</epage><pages>30-38</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>To compare the rate of chromosome abnormalities in embryos obtained from karyotypically normal patients with nonobstructive azoospermia undergoing testicular sperm extraction (TESE) to those from patients undergoing intracytoplasmic sperm injection (ICSI) with ejaculated sperm.
Retrospective analysis.
IVF centers.
Male partners had either nonobstructive zoospermia or oligospermia.
Preimplantation genetic diagnosis. Chromosome enumeration was performed by fluorescence in situ hybridization (FISH). Embryos classified as abnormal were reanalyzed to study mosaicism.
Chromosome abnormalities in embryos.
Embryos from ICSI cycles with ejaculated sperm (group 1) were 41.8% normal, 26.2% aneuploid, and 26.5% mosaic. In contrast, the embryos from ICSI cycles with TESE for nonobstructive azoospermia (group 2) were 22% normal, 17% aneuploid, and 53% mosaic. The difference in mosaicism rate between the two groups of embryos was highly significant.
The present study results indicate a high incidence of mosaicism in embryos derived from TESE in men with a severe deficit in spermatogenesis. Sperm derived from TESE for nonobstructive azoospermia may have a higher rate of compromised or immature centrosome structures leading to mosaicism in the embryo.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12524060</pmid><doi>10.1016/S0015-0282(02)04407-2</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aneuploidy Biological and medical sciences chaotic Chromosome Aberrations Humans In Situ Hybridization, Fluorescence Male Medical genetics Medical sciences Mosaicism Oligospermia - therapy PGD Preimplantation Diagnosis preimplantation genetic diagnosis Retrospective Studies Sperm Injections, Intracytoplasmic Spermatozoa Testis - cytology Tissue and Organ Harvesting - methods |
| title | Chromosomal abnormalities in embryos derived from testicular sperm extraction |
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