Comparison of TaqMan™ real-time PCR and p24 Elisa for quantification of in vitro HIV-1 replication

In this study, TaqMan™ PCR was used to assess viral replication of HIV-1 infected cells in vitro. This PCR technique was compared with p24 ELISA as a standard method to monitor HIV-1 replication in cell culture. Hut78 T-lymphoblastoid cells were infected with different titres of HIV-1 IIIb (MOI 0.05...

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Veröffentlicht in:	Journal of virological methods 2003-02, Vol.107 (2), p.169-175
Hauptverfasser:	Klein, Stefan A, Karsten, Susanne, Rüster, Brigitte, Klebba, Christian, Pape, Martine, Ottmann, Oliver G, Hoelzer, Dieter, Roth, Willi K
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell Line Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Gene Products, gag - metabolism HIV Core Protein p24 - metabolism HIV-1 - genetics HIV-1 - pathogenicity HIV-1 - physiology HIV-1 quantitation Humans Microbiology p24 ELISA Polymerase Chain Reaction - methods Real-time PCR RNA, Viral - analysis RT-PCR Sensitivity and Specificity Taq Polymerase - metabolism Techniques used in virology Virology Virus Replication
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