Recovery kinetics after chemotherapy and circulating mononuclear cells expressing the CD34 antigen in pediatric cancer patients
Hematopoietic progenitor cells collected from the peripheral blood are capable of restoring hematopoiesis after myeloablative therapy. The numbers of circulating mononuclear cells expressing the CD34 antigen were calculated and the colony-forming capacity was determined in 26 blood samples, which we...
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| Veröffentlicht in: | Annals of hematology 1992-04, Vol.64 (4), p.181-184 |
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| container_title | Annals of hematology |
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| creator | EMMINGER, W FRITSCH, G EMMINGER-SCHMIDMEIER, W BUCHINGER, P PRINTZ, D KUNDI, M GADNER, H |
| description | Hematopoietic progenitor cells collected from the peripheral blood are capable of restoring hematopoiesis after myeloablative therapy. The numbers of circulating mononuclear cells expressing the CD34 antigen were calculated and the colony-forming capacity was determined in 26 blood samples, which were drawn during rapid rise of leukocytes after chemotherapy cycles that were followed by aplasia. Culture assay after 14 days revealed a median 507 (210-2029) myeloid progenitors (CFU-GEMM/GM) per 10(5) nucleated cells (NC) in 13 CD34-positive samples, and only a median 76 (9-224) in 13 CD34-negative ones (p less than 0.001). Median 343 (175-2450) erythroid burst-forming units (BFU-E) per 10(5) NC were detected in the CD34-positive samples, whereas only 72 (10-315) per 10(5) NC were found in the negative ones (p less than 0.01). The percentage of CD34-positive cells clearly correlated with the growth of CFU-GEMM/GM and BFU-E (p less than 0.01). The content of CD34-positive cells in circulation was determined within 120 min by FACS analysis and predicted colony-forming capacity of circulating mononuclear cells. These observations will help to select the optimal individual days for leukaphereses. |
| doi_str_mv | 10.1007/BF01696220 |
| format | Article |
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The numbers of circulating mononuclear cells expressing the CD34 antigen were calculated and the colony-forming capacity was determined in 26 blood samples, which were drawn during rapid rise of leukocytes after chemotherapy cycles that were followed by aplasia. Culture assay after 14 days revealed a median 507 (210-2029) myeloid progenitors (CFU-GEMM/GM) per 10(5) nucleated cells (NC) in 13 CD34-positive samples, and only a median 76 (9-224) in 13 CD34-negative ones (p less than 0.001). Median 343 (175-2450) erythroid burst-forming units (BFU-E) per 10(5) NC were detected in the CD34-positive samples, whereas only 72 (10-315) per 10(5) NC were found in the negative ones (p less than 0.01). The percentage of CD34-positive cells clearly correlated with the growth of CFU-GEMM/GM and BFU-E (p less than 0.01). The content of CD34-positive cells in circulation was determined within 120 min by FACS analysis and predicted colony-forming capacity of circulating mononuclear cells. These observations will help to select the optimal individual days for leukaphereses.</description><identifier>ISSN: 0939-5555</identifier><identifier>EISSN: 1432-0584</identifier><identifier>DOI: 10.1007/BF01696220</identifier><identifier>PMID: 1374649</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Adolescent ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Antigens, CD - analysis ; Antigens, CD34 ; Biological and medical sciences ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; Child ; Child, Preschool ; Colony-Forming Units Assay ; Flow Cytometry ; Humans ; Infant ; Kinetics ; Leukapheresis ; Leukemia - blood ; Leukemia - drug therapy ; Leukocytes, Mononuclear - immunology ; Medical sciences ; Neuroblastoma - blood ; Neuroblastoma - drug therapy ; Regression Analysis ; Sarcoma, Ewing - blood ; Sarcoma, Ewing - drug therapy ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><ispartof>Annals of hematology, 1992-04, Vol.64 (4), p.181-184</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-fb557b5b7af3afd775d55a9dc4ac89ed754fd62cbab3cc9461d007f4f507d36f3</citedby><cites>FETCH-LOGICAL-c311t-fb557b5b7af3afd775d55a9dc4ac89ed754fd62cbab3cc9461d007f4f507d36f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5614495$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1374649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>EMMINGER, W</creatorcontrib><creatorcontrib>FRITSCH, G</creatorcontrib><creatorcontrib>EMMINGER-SCHMIDMEIER, W</creatorcontrib><creatorcontrib>BUCHINGER, P</creatorcontrib><creatorcontrib>PRINTZ, D</creatorcontrib><creatorcontrib>KUNDI, M</creatorcontrib><creatorcontrib>GADNER, H</creatorcontrib><title>Recovery kinetics after chemotherapy and circulating mononuclear cells expressing the CD34 antigen in pediatric cancer patients</title><title>Annals of hematology</title><addtitle>Ann Hematol</addtitle><description>Hematopoietic progenitor cells collected from the peripheral blood are capable of restoring hematopoiesis after myeloablative therapy. The numbers of circulating mononuclear cells expressing the CD34 antigen were calculated and the colony-forming capacity was determined in 26 blood samples, which were drawn during rapid rise of leukocytes after chemotherapy cycles that were followed by aplasia. Culture assay after 14 days revealed a median 507 (210-2029) myeloid progenitors (CFU-GEMM/GM) per 10(5) nucleated cells (NC) in 13 CD34-positive samples, and only a median 76 (9-224) in 13 CD34-negative ones (p less than 0.001). Median 343 (175-2450) erythroid burst-forming units (BFU-E) per 10(5) NC were detected in the CD34-positive samples, whereas only 72 (10-315) per 10(5) NC were found in the negative ones (p less than 0.01). The percentage of CD34-positive cells clearly correlated with the growth of CFU-GEMM/GM and BFU-E (p less than 0.01). The content of CD34-positive cells in circulation was determined within 120 min by FACS analysis and predicted colony-forming capacity of circulating mononuclear cells. These observations will help to select the optimal individual days for leukaphereses.</description><subject>Adolescent</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Antigens, CD - analysis</subject><subject>Antigens, CD34</subject><subject>Biological and medical sciences</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Colony-Forming Units Assay</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Infant</subject><subject>Kinetics</subject><subject>Leukapheresis</subject><subject>Leukemia - blood</subject><subject>Leukemia - drug therapy</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Medical sciences</subject><subject>Neuroblastoma - blood</subject><subject>Neuroblastoma - drug therapy</subject><subject>Regression Analysis</subject><subject>Sarcoma, Ewing - blood</subject><subject>Sarcoma, Ewing - drug therapy</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0939-5555</issn><issn>1432-0584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEFv1DAQhS1EVZbChTuSD4gDUqgd2_H6SBcKSJUqIThHznjcGhIn2A7qnvjreLUrOpc5vO89zTxCXnH2njOmL6-uGe9M17bsCdlwKdqGqa18SjbMCNOoOs_I85x_MsbbrWzPyTkXWnbSbMjfbwjzH0x7-itELAEytb5gonCP01zuMdllT210FEKCdbQlxDs6zXGOK4xoK4jjmCk-LAlzPojVRHcfhayuEu4w0hDpgi7YkgJQsBFq_FKDMJb8gpx5O2Z8edoX5Mf1p--7L83N7eevuw83DQjOS-MHpfSgBm29sN5prZxS1jiQFrYGnVbSu66FwQ4CwMiOu1qMl14x7UTnxQV5e8xd0vx7xVz6KeTD6TbivOZet0a2nRAVfHcEIc05J_T9ksJk077nrD-03T-2XeHXp9R1mNA9osd6q_7mpNsMdvSpPh_yf0x1XEqjxD-ic4mh</recordid><startdate>19920401</startdate><enddate>19920401</enddate><creator>EMMINGER, W</creator><creator>FRITSCH, G</creator><creator>EMMINGER-SCHMIDMEIER, W</creator><creator>BUCHINGER, P</creator><creator>PRINTZ, D</creator><creator>KUNDI, M</creator><creator>GADNER, H</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920401</creationdate><title>Recovery kinetics after chemotherapy and circulating mononuclear cells expressing the CD34 antigen in pediatric cancer patients</title><author>EMMINGER, W ; FRITSCH, G ; EMMINGER-SCHMIDMEIER, W ; BUCHINGER, P ; PRINTZ, D ; KUNDI, M ; GADNER, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-fb557b5b7af3afd775d55a9dc4ac89ed754fd62cbab3cc9461d007f4f507d36f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adolescent</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Antigens, CD - analysis</topic><topic>Antigens, CD34</topic><topic>Biological and medical sciences</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Colony-Forming Units Assay</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Infant</topic><topic>Kinetics</topic><topic>Leukapheresis</topic><topic>Leukemia - blood</topic><topic>Leukemia - drug therapy</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Medical sciences</topic><topic>Neuroblastoma - blood</topic><topic>Neuroblastoma - drug therapy</topic><topic>Regression Analysis</topic><topic>Sarcoma, Ewing - blood</topic><topic>Sarcoma, Ewing - drug therapy</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>EMMINGER, W</creatorcontrib><creatorcontrib>FRITSCH, G</creatorcontrib><creatorcontrib>EMMINGER-SCHMIDMEIER, W</creatorcontrib><creatorcontrib>BUCHINGER, P</creatorcontrib><creatorcontrib>PRINTZ, D</creatorcontrib><creatorcontrib>KUNDI, M</creatorcontrib><creatorcontrib>GADNER, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of hematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>EMMINGER, W</au><au>FRITSCH, G</au><au>EMMINGER-SCHMIDMEIER, W</au><au>BUCHINGER, P</au><au>PRINTZ, D</au><au>KUNDI, M</au><au>GADNER, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recovery kinetics after chemotherapy and circulating mononuclear cells expressing the CD34 antigen in pediatric cancer patients</atitle><jtitle>Annals of hematology</jtitle><addtitle>Ann Hematol</addtitle><date>1992-04-01</date><risdate>1992</risdate><volume>64</volume><issue>4</issue><spage>181</spage><epage>184</epage><pages>181-184</pages><issn>0939-5555</issn><eissn>1432-0584</eissn><abstract>Hematopoietic progenitor cells collected from the peripheral blood are capable of restoring hematopoiesis after myeloablative therapy. The numbers of circulating mononuclear cells expressing the CD34 antigen were calculated and the colony-forming capacity was determined in 26 blood samples, which were drawn during rapid rise of leukocytes after chemotherapy cycles that were followed by aplasia. Culture assay after 14 days revealed a median 507 (210-2029) myeloid progenitors (CFU-GEMM/GM) per 10(5) nucleated cells (NC) in 13 CD34-positive samples, and only a median 76 (9-224) in 13 CD34-negative ones (p less than 0.001). Median 343 (175-2450) erythroid burst-forming units (BFU-E) per 10(5) NC were detected in the CD34-positive samples, whereas only 72 (10-315) per 10(5) NC were found in the negative ones (p less than 0.01). The percentage of CD34-positive cells clearly correlated with the growth of CFU-GEMM/GM and BFU-E (p less than 0.01). The content of CD34-positive cells in circulation was determined within 120 min by FACS analysis and predicted colony-forming capacity of circulating mononuclear cells. These observations will help to select the optimal individual days for leukaphereses.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>1374649</pmid><doi>10.1007/BF01696220</doi><tpages>4</tpages></addata></record> |
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| subjects | Adolescent Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Antigens, CD - analysis Antigens, CD34 Biological and medical sciences Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Child Child, Preschool Colony-Forming Units Assay Flow Cytometry Humans Infant Kinetics Leukapheresis Leukemia - blood Leukemia - drug therapy Leukocytes, Mononuclear - immunology Medical sciences Neuroblastoma - blood Neuroblastoma - drug therapy Regression Analysis Sarcoma, Ewing - blood Sarcoma, Ewing - drug therapy Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | Recovery kinetics after chemotherapy and circulating mononuclear cells expressing the CD34 antigen in pediatric cancer patients |
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