Biological activities of osteoblasts on poly(methyl methacrylate)/silica hybrid containing calcium salt
The biological activity of osteoblasts on the newly developed bioactive poly(methyl methacrylate) (PMMA)/silica hybrid containing calcium salt was investigated. The attachment, proliferation, and differentiation of primary cultured mouse calvarial osteoblasts were evaluated by hexosaminase, MTT, and...
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| Veröffentlicht in: | Biomaterials 2003-03, Vol.24 (6), p.901-906 |
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| creator | Rhee, Sang-Hoon Hwang, Mi-Hye Si, Hyun-Jung Choi, Je-Yong |
| description | The biological activity of osteoblasts on the newly developed bioactive poly(methyl methacrylate) (PMMA)/silica hybrid containing calcium salt was investigated. The attachment, proliferation, and differentiation of primary cultured mouse calvarial osteoblasts were evaluated by hexosaminase, MTT, and alkaline phosphatase activity assays, respectively. The PMMA/silica hybrid showed higher biological activities than those of pure PMMA with regard to all three parameters. Besides, the calcium phosphate layer, determined by scanning electron microscopy with energy dispersive spectroscopy, occurred only on the PMMA/silica hybrid. Better biological activities on the PMMA/silica hybrid than those on the PMMA were explained by the role of calcium phosphate layer formed on the PMMA/silica hybrid and the released calcium and silicon ions from it during the cell culture. These results suggest that the PMMA/silica hybrid might be useful as a bone substitute or filler. |
| doi_str_mv | 10.1016/S0142-9612(02)00421-0 |
| format | Article |
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The attachment, proliferation, and differentiation of primary cultured mouse calvarial osteoblasts were evaluated by hexosaminase, MTT, and alkaline phosphatase activity assays, respectively. The PMMA/silica hybrid showed higher biological activities than those of pure PMMA with regard to all three parameters. Besides, the calcium phosphate layer, determined by scanning electron microscopy with energy dispersive spectroscopy, occurred only on the PMMA/silica hybrid. Better biological activities on the PMMA/silica hybrid than those on the PMMA were explained by the role of calcium phosphate layer formed on the PMMA/silica hybrid and the released calcium and silicon ions from it during the cell culture. 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The attachment, proliferation, and differentiation of primary cultured mouse calvarial osteoblasts were evaluated by hexosaminase, MTT, and alkaline phosphatase activity assays, respectively. The PMMA/silica hybrid showed higher biological activities than those of pure PMMA with regard to all three parameters. Besides, the calcium phosphate layer, determined by scanning electron microscopy with energy dispersive spectroscopy, occurred only on the PMMA/silica hybrid. Better biological activities on the PMMA/silica hybrid than those on the PMMA were explained by the role of calcium phosphate layer formed on the PMMA/silica hybrid and the released calcium and silicon ions from it during the cell culture. These results suggest that the PMMA/silica hybrid might be useful as a bone substitute or filler.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Alkaline phosphatase activity</subject><subject>Animals</subject><subject>Attachment</subject><subject>beta-N-Acetylhexosaminidases - metabolism</subject><subject>Calcium Phosphates</subject><subject>Cell Adhesion</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Cell Membrane - ultrastructure</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>Osteoblast</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - physiology</subject><subject>Osteoblasts - ultrastructure</subject><subject>Poly(methyl methacrylate)/silica</subject><subject>Polymethyl Methacrylate</subject><subject>Proliferation</subject><subject>Silicon Dioxide</subject><subject>Skull - cytology</subject><subject>Skull - physiology</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2LFDEQhoMo7rj6E5ScZPfQbuVrOjmJLn7Bggf1HNKZ6tlIujMmmYX-92acQY8jBIoiz1sF9RDyksEbBmx98w2Y5J1ZM34F_BpActbBI7JiutedMqAek9Vf5II8K-UntL5xT8kF4wqkYrAi2_chxbQN3kXqfA0PoQYsNI00lYppiK7U1s50l-JyNWG9XyI9FOfzEl3F65sSYovT-2XIYUN9mqsLc5i3tM30YT_R4mJ9Tp6MLhZ8caqX5MfHD99vP3d3Xz99uX1313nFoXZ88MqtDZdr6b0ZtWZaKj2Ac7xn3HAx6EGiYUI7znrje-VM-0SDXrTIKC7J6-PcXU6_9liqnULxGKObMe2L7bmRrBfqLMh7DUox9l-gFFKcBZsZIYzgDVRH0OdUSsbR7nKYXF4sA3uQa__ItQdzFto7yLXQcq9OC_bDhJt_qZPNBrw9Atgu_BAw2-IDzh43IaOvdpPCmRW_AboBtAY</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Rhee, Sang-Hoon</creator><creator>Hwang, Mi-Hye</creator><creator>Si, Hyun-Jung</creator><creator>Choi, Je-Yong</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>F28</scope><scope>JG9</scope><scope>7QQ</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Biological activities of osteoblasts on poly(methyl methacrylate)/silica hybrid containing calcium salt</title><author>Rhee, Sang-Hoon ; 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The attachment, proliferation, and differentiation of primary cultured mouse calvarial osteoblasts were evaluated by hexosaminase, MTT, and alkaline phosphatase activity assays, respectively. The PMMA/silica hybrid showed higher biological activities than those of pure PMMA with regard to all three parameters. Besides, the calcium phosphate layer, determined by scanning electron microscopy with energy dispersive spectroscopy, occurred only on the PMMA/silica hybrid. Better biological activities on the PMMA/silica hybrid than those on the PMMA were explained by the role of calcium phosphate layer formed on the PMMA/silica hybrid and the released calcium and silicon ions from it during the cell culture. These results suggest that the PMMA/silica hybrid might be useful as a bone substitute or filler.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>12504510</pmid><doi>10.1016/S0142-9612(02)00421-0</doi><tpages>6</tpages></addata></record> |
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| language | eng |
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| subjects | Alkaline Phosphatase - metabolism Alkaline phosphatase activity Animals Attachment beta-N-Acetylhexosaminidases - metabolism Calcium Phosphates Cell Adhesion Cell Culture Techniques - methods Cell Differentiation Cell Membrane - ultrastructure Mice Microscopy, Electron, Scanning Osteoblast Osteoblasts - cytology Osteoblasts - physiology Osteoblasts - ultrastructure Poly(methyl methacrylate)/silica Polymethyl Methacrylate Proliferation Silicon Dioxide Skull - cytology Skull - physiology |
| title | Biological activities of osteoblasts on poly(methyl methacrylate)/silica hybrid containing calcium salt |
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