Antibodies Elicited by a Cryptococcus neoformans-Tetanus Toxoid Conjugate Vaccine Have the Same Specificity as Those Elicited in Infection
The antibody responses of BALB/c mice to serotype A Cryptococcus neoformans capsular polysaccharide (CNPS) were compared after cryptococcal infection and immunization with a serotype A glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT). Infection rarely resulted in a rise of serum antibody titer...
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| Veröffentlicht in: | The Journal of infectious diseases 1992-06, Vol.165 (6), p.1086-1093 |
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| container_title | The Journal of infectious diseases |
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| creator | Casadevall, Arturo Mukherjee, Jean Devi, Sarvamangala J. N. Schneerson, Rachel Robbins, John B. Scharff, Matthew D. |
| description | The antibody responses of BALB/c mice to serotype A Cryptococcus neoformans capsular polysaccharide (CNPS) were compared after cryptococcal infection and immunization with a serotype A glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT). Infection rarely resulted in a rise of serum antibody titer to CNPS. In contrast, mice immunized with GXM-TT produced serum IgM and IgG to CNPS. Six IgM and one IgG1 monoclonal antibodies (MAbs) were generated from the spleen of one infected mouse. Nine IgM, 1 IgG3, 16 IgGI, and 7 IgA MAbs were generated from the spleen of one GXM-TT-immunized mouse. All MAbs generated from both mice bound to the GXM fraction of the capsular polysaccharide. For some MAbs, de-O-acetylation of serotype A GXM abolished or greatly reduced MAb binding compared with the native GXM. All MAbs reacted with CNPS from C. neoformans serotypes A-D. MAbs generated from the infected mouse competitively inhibited the binding of MAbs generated from the GXM-TT-immunized mouse. These results indicate that some antibodies elicited by infection with C. neoformans or by immunization with GXM-TT bind to the same antigenic determinant in the GXM. |
| doi_str_mv | 10.1093/infdis/165.6.1086 |
| format | Article |
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For some MAbs, de-O-acetylation of serotype A GXM abolished or greatly reduced MAb binding compared with the native GXM. All MAbs reacted with CNPS from C. neoformans serotypes A-D. MAbs generated from the infected mouse competitively inhibited the binding of MAbs generated from the GXM-TT-immunized mouse. These results indicate that some antibodies elicited by infection with C. neoformans or by immunization with GXM-TT bind to the same antigenic determinant in the GXM.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/165.6.1086</identifier><identifier>PMID: 1583327</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>AIDS ; Animals ; Antibodies ; Antibodies, Fungal - biosynthesis ; Antibodies, Fungal - blood ; Antibodies, Monoclonal - immunology ; Antibody Specificity ; Antigens, Fungal - immunology ; Binding, Competitive ; Biological and medical sciences ; Clostridium tetani ; Cryptococcosis - immunology ; Cryptococcus neoformans ; Cryptococcus neoformans - immunology ; Enzyme linked immunosorbent assay ; Epitopes ; Fungal Vaccines - immunology ; Human mycoses ; Hybridomas ; Immunization ; Immunoglobulin G - biosynthesis ; Immunoglobulin G - blood ; Immunoglobulin M - biosynthesis ; Immunoglobulin M - blood ; Infections ; Infectious diseases ; Major Articles ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Miscellaneous mycoses ; Monoclonal antibodies ; Mycoses ; Polysaccharides ; Polysaccharides - immunology ; Tetanus Toxoid - immunology ; Vaccines, Synthetic - immunology</subject><ispartof>The Journal of infectious diseases, 1992-06, Vol.165 (6), p.1086-1093</ispartof><rights>Copyright 1992 The University of Chicago</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-23daaa7f792a9be445cdc2af131c9d0a3ca1324b6655b1bcd5ae7f8b63f55bb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30112194$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30112194$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5497617$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1583327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Casadevall, Arturo</creatorcontrib><creatorcontrib>Mukherjee, Jean</creatorcontrib><creatorcontrib>Devi, Sarvamangala J. N.</creatorcontrib><creatorcontrib>Schneerson, Rachel</creatorcontrib><creatorcontrib>Robbins, John B.</creatorcontrib><creatorcontrib>Scharff, Matthew D.</creatorcontrib><title>Antibodies Elicited by a Cryptococcus neoformans-Tetanus Toxoid Conjugate Vaccine Have the Same Specificity as Those Elicited in Infection</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>The antibody responses of BALB/c mice to serotype A Cryptococcus neoformans capsular polysaccharide (CNPS) were compared after cryptococcal infection and immunization with a serotype A glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT). Infection rarely resulted in a rise of serum antibody titer to CNPS. In contrast, mice immunized with GXM-TT produced serum IgM and IgG to CNPS. Six IgM and one IgG1 monoclonal antibodies (MAbs) were generated from the spleen of one infected mouse. Nine IgM, 1 IgG3, 16 IgGI, and 7 IgA MAbs were generated from the spleen of one GXM-TT-immunized mouse. All MAbs generated from both mice bound to the GXM fraction of the capsular polysaccharide. For some MAbs, de-O-acetylation of serotype A GXM abolished or greatly reduced MAb binding compared with the native GXM. All MAbs reacted with CNPS from C. neoformans serotypes A-D. MAbs generated from the infected mouse competitively inhibited the binding of MAbs generated from the GXM-TT-immunized mouse. These results indicate that some antibodies elicited by infection with C. neoformans or by immunization with GXM-TT bind to the same antigenic determinant in the GXM.</description><subject>AIDS</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Fungal - biosynthesis</subject><subject>Antibodies, Fungal - blood</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>Antigens, Fungal - immunology</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Clostridium tetani</subject><subject>Cryptococcosis - immunology</subject><subject>Cryptococcus neoformans</subject><subject>Cryptococcus neoformans - immunology</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Epitopes</subject><subject>Fungal Vaccines - immunology</subject><subject>Human mycoses</subject><subject>Hybridomas</subject><subject>Immunization</subject><subject>Immunoglobulin G - biosynthesis</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin M - biosynthesis</subject><subject>Immunoglobulin M - blood</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Major Articles</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Miscellaneous mycoses</subject><subject>Monoclonal antibodies</subject><subject>Mycoses</subject><subject>Polysaccharides</subject><subject>Polysaccharides - immunology</subject><subject>Tetanus Toxoid - immunology</subject><subject>Vaccines, Synthetic - immunology</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAURSMEKkPhA1ggeYHYpY3t2E6WZShMUSUWHaGqG-vFsamHxB5sB3V-oV9djzJ0lmxs6d17z7N1i-I9rs5w1dJz60xv4znm7IznScNfFAvMqCg5x_RlsagqQkrctO3r4k2Mm6qqasrFSXGCWUMpEYvi8cIl2_ne6oguB6ts0j3qdgjQMuy2ySuv1BSR0974MIKL5VoncHm09g_e9mjp3Wb6BUmjn6CUdRqt4K9G6V6jGxjzsdXKmj04Q3Pq3kd93GQdunJGq2S9e1u8MjBE_e5wnxbrr5fr5aq8_vHtanlxXaq6IakktAcAYURLoO10XTPVKwIGU6zavgKqAFNSd5wz1uFO9Qy0ME3HqcmDjp4Wn2bsNvg_k45JjjYqPQyQPzlFKUhLGRfiv0bMKa25qLMRz0YVfIxBG7kNdoSwk7iS-57k3FNOMMnlvqec-XCAT92o-2NiLibrHw86RAWDCeBUJvyzsboVHIsjZhOTD88yrTAmuN0_rZx1G5N-eNYh_JZcUMHk6vZOfrldsbub77X8TJ8AuoO4CQ</recordid><startdate>19920601</startdate><enddate>19920601</enddate><creator>Casadevall, Arturo</creator><creator>Mukherjee, Jean</creator><creator>Devi, Sarvamangala J. 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N. ; Schneerson, Rachel ; Robbins, John B. ; Scharff, Matthew D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-23daaa7f792a9be445cdc2af131c9d0a3ca1324b6655b1bcd5ae7f8b63f55bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>AIDS</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Fungal - biosynthesis</topic><topic>Antibodies, Fungal - blood</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity</topic><topic>Antigens, Fungal - immunology</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Clostridium tetani</topic><topic>Cryptococcosis - immunology</topic><topic>Cryptococcus neoformans</topic><topic>Cryptococcus neoformans - immunology</topic><topic>Enzyme linked immunosorbent assay</topic><topic>Epitopes</topic><topic>Fungal Vaccines - immunology</topic><topic>Human mycoses</topic><topic>Hybridomas</topic><topic>Immunization</topic><topic>Immunoglobulin G - biosynthesis</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin M - biosynthesis</topic><topic>Immunoglobulin M - blood</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Major Articles</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Miscellaneous mycoses</topic><topic>Monoclonal antibodies</topic><topic>Mycoses</topic><topic>Polysaccharides</topic><topic>Polysaccharides - immunology</topic><topic>Tetanus Toxoid - immunology</topic><topic>Vaccines, Synthetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casadevall, Arturo</creatorcontrib><creatorcontrib>Mukherjee, Jean</creatorcontrib><creatorcontrib>Devi, Sarvamangala J. N.</creatorcontrib><creatorcontrib>Schneerson, Rachel</creatorcontrib><creatorcontrib>Robbins, John B.</creatorcontrib><creatorcontrib>Scharff, Matthew D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casadevall, Arturo</au><au>Mukherjee, Jean</au><au>Devi, Sarvamangala J. N.</au><au>Schneerson, Rachel</au><au>Robbins, John B.</au><au>Scharff, Matthew D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibodies Elicited by a Cryptococcus neoformans-Tetanus Toxoid Conjugate Vaccine Have the Same Specificity as Those Elicited in Infection</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1992-06-01</date><risdate>1992</risdate><volume>165</volume><issue>6</issue><spage>1086</spage><epage>1093</epage><pages>1086-1093</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>The antibody responses of BALB/c mice to serotype A Cryptococcus neoformans capsular polysaccharide (CNPS) were compared after cryptococcal infection and immunization with a serotype A glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT). Infection rarely resulted in a rise of serum antibody titer to CNPS. In contrast, mice immunized with GXM-TT produced serum IgM and IgG to CNPS. Six IgM and one IgG1 monoclonal antibodies (MAbs) were generated from the spleen of one infected mouse. Nine IgM, 1 IgG3, 16 IgGI, and 7 IgA MAbs were generated from the spleen of one GXM-TT-immunized mouse. All MAbs generated from both mice bound to the GXM fraction of the capsular polysaccharide. For some MAbs, de-O-acetylation of serotype A GXM abolished or greatly reduced MAb binding compared with the native GXM. All MAbs reacted with CNPS from C. neoformans serotypes A-D. MAbs generated from the infected mouse competitively inhibited the binding of MAbs generated from the GXM-TT-immunized mouse. These results indicate that some antibodies elicited by infection with C. neoformans or by immunization with GXM-TT bind to the same antigenic determinant in the GXM.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>1583327</pmid><doi>10.1093/infdis/165.6.1086</doi><tpages>8</tpages></addata></record> |
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| ispartof | The Journal of infectious diseases, 1992-06, Vol.165 (6), p.1086-1093 |
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| subjects | AIDS Animals Antibodies Antibodies, Fungal - biosynthesis Antibodies, Fungal - blood Antibodies, Monoclonal - immunology Antibody Specificity Antigens, Fungal - immunology Binding, Competitive Biological and medical sciences Clostridium tetani Cryptococcosis - immunology Cryptococcus neoformans Cryptococcus neoformans - immunology Enzyme linked immunosorbent assay Epitopes Fungal Vaccines - immunology Human mycoses Hybridomas Immunization Immunoglobulin G - biosynthesis Immunoglobulin G - blood Immunoglobulin M - biosynthesis Immunoglobulin M - blood Infections Infectious diseases Major Articles Medical sciences Mice Mice, Inbred BALB C Miscellaneous mycoses Monoclonal antibodies Mycoses Polysaccharides Polysaccharides - immunology Tetanus Toxoid - immunology Vaccines, Synthetic - immunology |
| title | Antibodies Elicited by a Cryptococcus neoformans-Tetanus Toxoid Conjugate Vaccine Have the Same Specificity as Those Elicited in Infection |
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