Purification and properties of a phytase from Candida krusei WZ-001

A phytase from Candida krusei WZ-001 isolated from soil was purified to electrophoretic homogeneity by ion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration. The phytase is composed of two different subunits with molecular masses of 116 kDa and 31 kDa on SDS-PAGE (o...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2002, Vol.94 (5), p.419-425
Hauptverfasser:	Quan, Chun-Shan, Fan, Sheng-Di, Zhang, Ling-Hua, Wang, Yun-Ji, Ohta, Yoshiyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology CANDIDA Candida krusei Enzyme engineering Fundamental and applied biological sciences. Psychology Improved methods for extraction and purification of enzymes Methods. Procedures. Technologies microbial phytase phosphate PHYTASE phytate degradation PHYTATES PURIFICATION
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