Identification and isolation of the Leishmania transferrin receptor

In a previous report, we have presented several lines of evidence, derived from widely different methodologies, suggesting that Leishmania has specific receptors for transferrin with a Kd similar to the mammalian transferrin receptor. This paper describes the identification, purification, and bioche...

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Veröffentlicht in:The Journal of biological chemistry 1992-05, Vol.267 (13), p.9112-9117
Hauptverfasser: VOYIATZAKI, C. S, SOTERIADOU, K. P
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container_title The Journal of biological chemistry
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creator VOYIATZAKI, C. S
SOTERIADOU, K. P
description In a previous report, we have presented several lines of evidence, derived from widely different methodologies, suggesting that Leishmania has specific receptors for transferrin with a Kd similar to the mammalian transferrin receptor. This paper describes the identification, purification, and biochemical characterization of Leishmania transferrin receptor. The Leishmania transferrin receptor, detected on intact parasites by immunoperoxidase staining, was first identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western blot analysis, using 125I-transferrin, as a 70-kDa protein. It has been isolated initially from Leishmania infantum promastigotes using affinity chromatography on a transferrin-Sepharose column and, subsequently, from Leishmania major promastigotes. The use of polyclonal antisera to the purified 70-kDa Leishmania transferrin receptor and to the purified rat transferrin receptor showed that the two receptors are antigenically distinct. The 70-kDa Leishmania transferrin receptor was subsequently characterized as an integral membrane glycoprotein. The monomeric state of the Leishmania transferrin receptor was demonstrated by gel filtration of purified receptor complexed with 125I-transferrin. Thus, the Leishmania transferrin receptor, unlike the mammalian receptor, is not a disulfide-linked dimer but a single 70-kDa polypeptide.
doi_str_mv 10.1016/s0021-9258(19)50396-9
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source MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Animals
Biological and medical sciences
Blotting, Western
Cell receptors
Cell structures and functions
Chromatography, Gel
Cross-Linking Reagents
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Humans
identification
Immunoenzyme Techniques
isolation
Leishmania
Leishmania - chemistry
Leishmania donovani - chemistry
membrane proteins
Miscellaneous
Molecular and cellular biology
Radioimmunoassay
Rats
receptors
Receptors, Transferrin - isolation & purification
transferrin
title Identification and isolation of the Leishmania transferrin receptor
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