Distribution of intracellular pH in the rat brain cortex after global ischemia as measured by color film histophotometry of neutral red
Tissue acidosis is an important determinant of cell viability following cerebral ischemia. Because of the heterogeneity of tissue response to metabolic stress, a method for measuring intracellular pH (pH i) that preserves spatil information would be desirable. Histophotometry of the pH indicator dye...
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| Veröffentlicht in: | Brain research 1992-02, Vol.573 (1), p.1-7 |
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| creator | Griffith, J. Keven Cordisco, Boris R. Lin, Chii-Wann LaManna, Joseph C. |
| description | Tissue acidosis is an important determinant of cell viability following cerebral ischemia. Because of the heterogeneity of tissue response to metabolic stress, a method for measuring intracellular pH (pH
i) that preserves spatil information would be desirable. Histophotometry of the pH indicator dye Neutral red offers such a possibility. The purpose of our study was to determine the distribution of pH
i following complete irreversible ischemia and show the correlation of mean pH
i measured by Neutral red and [
31P]NMR in the same brain. Three rats were studied in the anesthetized state. A pH
i range was obtained by total cerebral ischemia at various pre-arrest plasma glucose concentrations. The data show that mean pH
i calculated by Neutral red was strongly correlated to pH
i determined from [
31P]NMR (slope:0.99 ± 0.08;
P < 0.001, r
2 = 0.96). Within each brain, 80–110 discrete samples were analyzed by histophotometry. The pH
i distribution of those samples broadened in those rat brains with greater acidosis, suggesting a heterogeneity of response by the tissue to ischemia and the presence of multiple pH
i pools. Our results demonstrate the need to use methods which maintain spatial resolution such as is available with histophotometry. |
| doi_str_mv | 10.1016/0006-8993(92)90108-L |
| format | Article |
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i) that preserves spatil information would be desirable. Histophotometry of the pH indicator dye Neutral red offers such a possibility. The purpose of our study was to determine the distribution of pH
i following complete irreversible ischemia and show the correlation of mean pH
i measured by Neutral red and [
31P]NMR in the same brain. Three rats were studied in the anesthetized state. A pH
i range was obtained by total cerebral ischemia at various pre-arrest plasma glucose concentrations. The data show that mean pH
i calculated by Neutral red was strongly correlated to pH
i determined from [
31P]NMR (slope:0.99 ± 0.08;
P < 0.001, r
2 = 0.96). Within each brain, 80–110 discrete samples were analyzed by histophotometry. The pH
i distribution of those samples broadened in those rat brains with greater acidosis, suggesting a heterogeneity of response by the tissue to ischemia and the presence of multiple pH
i pools. Our results demonstrate the need to use methods which maintain spatial resolution such as is available with histophotometry.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(92)90108-L</identifier><identifier>PMID: 1576526</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>[ 31P]NMR ; Animals ; Biological and medical sciences ; Blood Glucose - metabolism ; Brain - pathology ; Brain Ischemia - metabolism ; Brain Ischemia - pathology ; Cerebral cortex ; Cerebral Cortex - metabolism ; Cerebral Cortex - pathology ; Histocytochemistry ; Hydrogen-Ion Concentration ; Intracellular pH ; Magnetic Resonance Spectroscopy ; Male ; Medical sciences ; Nervous system involvement in other diseases. Miscellaneous ; Neurology ; Neutral Red ; pH i ; Rats ; Rats, Inbred Strains ; Total cerebral ischemia</subject><ispartof>Brain research, 1992-02, Vol.573 (1), p.1-7</ispartof><rights>1992 Elsevier Science Publishers B.V. All rights reserved</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-e22df002b7b6185cedd24adb92165db0c7782e6b65b71dd1c8f600db8259d31b3</citedby><cites>FETCH-LOGICAL-c417t-e22df002b7b6185cedd24adb92165db0c7782e6b65b71dd1c8f600db8259d31b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-8993(92)90108-L$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5110070$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1576526$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Griffith, J. Keven</creatorcontrib><creatorcontrib>Cordisco, Boris R.</creatorcontrib><creatorcontrib>Lin, Chii-Wann</creatorcontrib><creatorcontrib>LaManna, Joseph C.</creatorcontrib><title>Distribution of intracellular pH in the rat brain cortex after global ischemia as measured by color film histophotometry of neutral red</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Tissue acidosis is an important determinant of cell viability following cerebral ischemia. Because of the heterogeneity of tissue response to metabolic stress, a method for measuring intracellular pH (pH
i) that preserves spatil information would be desirable. Histophotometry of the pH indicator dye Neutral red offers such a possibility. The purpose of our study was to determine the distribution of pH
i following complete irreversible ischemia and show the correlation of mean pH
i measured by Neutral red and [
31P]NMR in the same brain. Three rats were studied in the anesthetized state. A pH
i range was obtained by total cerebral ischemia at various pre-arrest plasma glucose concentrations. The data show that mean pH
i calculated by Neutral red was strongly correlated to pH
i determined from [
31P]NMR (slope:0.99 ± 0.08;
P < 0.001, r
2 = 0.96). Within each brain, 80–110 discrete samples were analyzed by histophotometry. The pH
i distribution of those samples broadened in those rat brains with greater acidosis, suggesting a heterogeneity of response by the tissue to ischemia and the presence of multiple pH
i pools. Our results demonstrate the need to use methods which maintain spatial resolution such as is available with histophotometry.</description><subject>[ 31P]NMR</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - metabolism</subject><subject>Brain - pathology</subject><subject>Brain Ischemia - metabolism</subject><subject>Brain Ischemia - pathology</subject><subject>Cerebral cortex</subject><subject>Cerebral Cortex - metabolism</subject><subject>Cerebral Cortex - pathology</subject><subject>Histocytochemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intracellular pH</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Nervous system involvement in other diseases. Miscellaneous</subject><subject>Neurology</subject><subject>Neutral Red</subject><subject>pH i</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Total cerebral ischemia</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-O1DAMxiMEWmYX3gCkHBCCQyHOtGlzQULLwiKNxAXOUf64TFDaDEmKmCfgtUmZ0XKDU-T4Z3-2P0KeAHsFDMRrxphoBim3LyR_KRmwodndIxsYet4I3rL7ZHOHPCSXOX-r4XYr2QW5gK4XHRcb8uudzyV5sxQfZxpH6ueStMUQlqATPdzWD1r2SJMu1CRdIxtTwZ9UjwUT_Rqi0YH6bPc4eU11phPqvCR01BwrG2Kiow8T3VeheNjHEics6bhqzbhUsUAr_Ig8GHXI-Pj8XpEv728-X982u08fPl6_3TW2hb40yLkbGeOmNwKGzqJzvNXOSA6ic4bZvh84CiM604NzYIdRMObMwDvptmC2V-T5qe8hxe8L5qKmOntdV88Yl6x6LjkfgP0XBAEShlZWsD2BNsWcE47qkPyk01EBU6tRanVBrS4oydUfo9Sulj0991_MhO5v0cmZmn92zutsdRiTnq3Pd1gHwFi_jvnmhGE92g-PSWXrca6H8QltUS76f8_xG9hlsVI</recordid><startdate>19920221</startdate><enddate>19920221</enddate><creator>Griffith, J. Keven</creator><creator>Cordisco, Boris R.</creator><creator>Lin, Chii-Wann</creator><creator>LaManna, Joseph C.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19920221</creationdate><title>Distribution of intracellular pH in the rat brain cortex after global ischemia as measured by color film histophotometry of neutral red</title><author>Griffith, J. Keven ; Cordisco, Boris R. ; Lin, Chii-Wann ; LaManna, Joseph C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-e22df002b7b6185cedd24adb92165db0c7782e6b65b71dd1c8f600db8259d31b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>[ 31P]NMR</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - metabolism</topic><topic>Brain - pathology</topic><topic>Brain Ischemia - metabolism</topic><topic>Brain Ischemia - pathology</topic><topic>Cerebral cortex</topic><topic>Cerebral Cortex - metabolism</topic><topic>Cerebral Cortex - pathology</topic><topic>Histocytochemistry</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intracellular pH</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Nervous system involvement in other diseases. Miscellaneous</topic><topic>Neurology</topic><topic>Neutral Red</topic><topic>pH i</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Total cerebral ischemia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Griffith, J. Keven</creatorcontrib><creatorcontrib>Cordisco, Boris R.</creatorcontrib><creatorcontrib>Lin, Chii-Wann</creatorcontrib><creatorcontrib>LaManna, Joseph C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Griffith, J. Keven</au><au>Cordisco, Boris R.</au><au>Lin, Chii-Wann</au><au>LaManna, Joseph C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of intracellular pH in the rat brain cortex after global ischemia as measured by color film histophotometry of neutral red</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1992-02-21</date><risdate>1992</risdate><volume>573</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Tissue acidosis is an important determinant of cell viability following cerebral ischemia. Because of the heterogeneity of tissue response to metabolic stress, a method for measuring intracellular pH (pH
i) that preserves spatil information would be desirable. Histophotometry of the pH indicator dye Neutral red offers such a possibility. The purpose of our study was to determine the distribution of pH
i following complete irreversible ischemia and show the correlation of mean pH
i measured by Neutral red and [
31P]NMR in the same brain. Three rats were studied in the anesthetized state. A pH
i range was obtained by total cerebral ischemia at various pre-arrest plasma glucose concentrations. The data show that mean pH
i calculated by Neutral red was strongly correlated to pH
i determined from [
31P]NMR (slope:0.99 ± 0.08;
P < 0.001, r
2 = 0.96). Within each brain, 80–110 discrete samples were analyzed by histophotometry. The pH
i distribution of those samples broadened in those rat brains with greater acidosis, suggesting a heterogeneity of response by the tissue to ischemia and the presence of multiple pH
i pools. Our results demonstrate the need to use methods which maintain spatial resolution such as is available with histophotometry.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>1576526</pmid><doi>10.1016/0006-8993(92)90108-L</doi><tpages>7</tpages></addata></record> |
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| subjects | [ 31P]NMR Animals Biological and medical sciences Blood Glucose - metabolism Brain - pathology Brain Ischemia - metabolism Brain Ischemia - pathology Cerebral cortex Cerebral Cortex - metabolism Cerebral Cortex - pathology Histocytochemistry Hydrogen-Ion Concentration Intracellular pH Magnetic Resonance Spectroscopy Male Medical sciences Nervous system involvement in other diseases. Miscellaneous Neurology Neutral Red pH i Rats Rats, Inbred Strains Total cerebral ischemia |
| title | Distribution of intracellular pH in the rat brain cortex after global ischemia as measured by color film histophotometry of neutral red |
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