Tangential flow filtration technology applicable to large scale recovery of diphtheria toxin
The commercial preparation of purified diphtheria toxoid primarily depends on the effective separation of the toxin from a large culture volume of Corynebacterium diphtheriae vaccine strain. Tangential flow or cross-flow filtration is gaining momentum in the processing of biologicals. This study dea...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2002, Vol.94 (2), p.93-98 |
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| container_end_page | 98 |
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| container_issue | 2 |
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| container_title | Journal of bioscience and bioengineering |
| container_volume | 94 |
| creator | Sundaran, Bheeman Palaniappan, Chitrambalam Rao, Yarlagadda Udaya Bhaskara Boopathy, Ratnam Rao Bhau, Lakshman Narasingha |
| description | The commercial preparation of purified diphtheria toxoid primarily depends on the effective separation of the toxin from a large culture volume of
Corynebacterium diphtheriae vaccine strain. Tangential flow or cross-flow filtration is gaining momentum in the processing of biologicals. This study deals with the separation of toxins using a microporous membrane (0.45-μm pore size) with operational variables like transmembrane pressure (TMP), cross flow rate and flux. Under the best conditions, ⪢98% recovery was achieved. The data and conclusions presented here have been drawn from process development work employing available equipment. |
| doi_str_mv | 10.1016/S1389-1723(02)80126-8 |
| format | Article |
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| identifier | ISSN: 1389-1723 |
| ispartof | Journal of bioscience and bioengineering, 2002, Vol.94 (2), p.93-98 |
| issn | 1389-1723 1347-4421 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Biological and medical sciences Biotechnology diphtheria toxin filter aid flux Fundamental and applied biological sciences. Psychology metafiltration Methods. Procedures. Technologies microfiltration module Others tangential flow filtration toxicity assay Various methods and equipments |
| title | Tangential flow filtration technology applicable to large scale recovery of diphtheria toxin |
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