Expression of human ferredoxin in Saccharomyces cerevisiae: Mitochondrial import of the protein and assembly of the [2Fe-2S] center

Vertebrate ferredoxins function in the transfer of reducing equivalents from NADPH:ferredoxin oxidoreductase to cytochrome P450 enzymes involved in steroid metabolism. We report here the expression of human mitochondrial ferredoxin in the yeast Saccharomyces cerevisiae. The full-length ferredoxin pr...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1992-05, Vol.294 (2), p.603-608
Hauptverfasser:	Seaton, Brent L., Vickery, Larry E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Biological and medical sciences complexes Electron Transport Complex IV - biosynthesis Electron Transport Complex IV - genetics Electron Transport Complex IV - isolation & purification Electrophoresis, Polyacrylamide Gel expression ferredoxin Ferredoxins - biosynthesis Ferredoxins - genetics Ferredoxins - isolation & purification formation Fundamental and applied biological sciences. Psychology Genes, Fungal Humans iron Kinetics Metalloproteins mitochondria Mitochondria - metabolism Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides Other metalloproteins Plasmids Promoter Regions, Genetic Protein Sorting Signals - genetics Proteins Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - isolation & purification Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Spectrophotometry uptake
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