A direct dot-enzyme immunoassay to detect human ovulation
This paper describes an original dot-enzyme-linked immunosorbent assay (ELISA) for predicting ovulation in women, based on the detection of the pre-ovulatory estrogen peak in urine. A monoclonal anti-estrogen antibody is used which recognizes not only free estrogens but also some of their urinary me...
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| Veröffentlicht in: | The Journal of steroid biochemistry and molecular biology 1992-04, Vol.42 (2), p.223-228 |
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| container_issue | 2 |
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| container_title | The Journal of steroid biochemistry and molecular biology |
| container_volume | 42 |
| creator | DE LAUZON, S DESFOSSES, B CHRISTEFF, N HANQUEZ, C CITTANOVA, N |
| description | This paper describes an original dot-enzyme-linked immunosorbent assay (ELISA) for predicting ovulation in women, based on the detection of the pre-ovulatory estrogen peak in urine. A monoclonal anti-estrogen antibody is used which recognizes not only free estrogens but also some of their urinary metabolites (17-glucuro- and sulfo-conjugates) allowing a direct assay on early morning urines. Antigen is immobilized as a spot on a nitrocellulose membrane which is immersed in urine in the presence of this antibody. A peroxidase-labeled second antibody allows the detection of the first antibody bound to the membrane. Antigen and anti-estrogen antibody concentrations are chosen to obtain a maximal enzymatic coloration of spots corresponding to basal urinary estrogen levels and no coloration corresponding to the pre-ovulatory surge. Six menstrual cycles were studied, comparing dot-ELISA results with patterns of: (1) urinary estrogens measured by RIA either directly or after hydrolysis and extraction, and (2) basal body temperatures. The validity of the pre-ovulatory signal obtained and the requirements for an adaptation of this methodology to a reliable home kit are discussed. |
| doi_str_mv | 10.1016/0960-0760(92)90031-D |
| format | Article |
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A monoclonal anti-estrogen antibody is used which recognizes not only free estrogens but also some of their urinary metabolites (17-glucuro- and sulfo-conjugates) allowing a direct assay on early morning urines. Antigen is immobilized as a spot on a nitrocellulose membrane which is immersed in urine in the presence of this antibody. A peroxidase-labeled second antibody allows the detection of the first antibody bound to the membrane. Antigen and anti-estrogen antibody concentrations are chosen to obtain a maximal enzymatic coloration of spots corresponding to basal urinary estrogen levels and no coloration corresponding to the pre-ovulatory surge. Six menstrual cycles were studied, comparing dot-ELISA results with patterns of: (1) urinary estrogens measured by RIA either directly or after hydrolysis and extraction, and (2) basal body temperatures. The validity of the pre-ovulatory signal obtained and the requirements for an adaptation of this methodology to a reliable home kit are discussed.</description><identifier>ISSN: 0960-0760</identifier><identifier>EISSN: 1879-1220</identifier><identifier>DOI: 10.1016/0960-0760(92)90031-D</identifier><identifier>PMID: 1567785</identifier><language>eng</language><publisher>Oxford: Elsevier Science</publisher><subject>Antibodies, Monoclonal ; Antibody Affinity ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay ; Estrogens - immunology ; Estrogens - urine ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrolysis ; Mammalian female genital system ; Morphology. 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A monoclonal anti-estrogen antibody is used which recognizes not only free estrogens but also some of their urinary metabolites (17-glucuro- and sulfo-conjugates) allowing a direct assay on early morning urines. Antigen is immobilized as a spot on a nitrocellulose membrane which is immersed in urine in the presence of this antibody. A peroxidase-labeled second antibody allows the detection of the first antibody bound to the membrane. Antigen and anti-estrogen antibody concentrations are chosen to obtain a maximal enzymatic coloration of spots corresponding to basal urinary estrogen levels and no coloration corresponding to the pre-ovulatory surge. Six menstrual cycles were studied, comparing dot-ELISA results with patterns of: (1) urinary estrogens measured by RIA either directly or after hydrolysis and extraction, and (2) basal body temperatures. The validity of the pre-ovulatory signal obtained and the requirements for an adaptation of this methodology to a reliable home kit are discussed.</description><subject>Antibodies, Monoclonal</subject><subject>Antibody Affinity</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Estrogens - immunology</subject><subject>Estrogens - urine</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Mammalian female genital system</subject><subject>Morphology. Physiology</subject><subject>Ovulation Detection</subject><subject>Radioimmunoassay</subject><subject>Reagent Kits, Diagnostic</subject><subject>Vertebrates: reproduction</subject><issn>0960-0760</issn><issn>1879-1220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLw0AUhQdRaq3-A4UsRHQRvfPITGdZWl9QcKPr4XYyg5EkUzOJUH-9iS116eouzncO3I-Qcwq3FKi8Ay0hBSXhWrMbDcBpujggYzpVOqWMwSEZ75FjchLjB_QQp2pERjSTSk2zMdGzJC8aZ9skD23q6u9N5ZKiqro6YIy4SdqQ5K4dgPeuwjoJX12JbRHqU3LksYzubHcn5O3h_nX-lC5fHp_ns2VquWRtmuVecBRae2qt9FNtPQjrpOOQ8Qyl5kzmHEGAtlLhisupFqxHhWeY5cgn5Gq7u27CZ-dia6oiWleWWLvQRaOYBkX7z_4DqeQgmMp6UGxB24QYG-fNuikqbDaGghnUmsGbGbwZzcyvWrPoaxe7_W5VufyvtHXZ55e7HKPF0jdY2yLusf5bSjPBfwCxHX_M</recordid><startdate>19920401</startdate><enddate>19920401</enddate><creator>DE LAUZON, S</creator><creator>DESFOSSES, B</creator><creator>CHRISTEFF, N</creator><creator>HANQUEZ, C</creator><creator>CITTANOVA, N</creator><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19920401</creationdate><title>A direct dot-enzyme immunoassay to detect human ovulation</title><author>DE LAUZON, S ; DESFOSSES, B ; CHRISTEFF, N ; HANQUEZ, C ; CITTANOVA, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-5df43a499f1cc6f89cf04ce6e30535a69326d3a0409c67ab368942cc64f2a5da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Antibodies, Monoclonal</topic><topic>Antibody Affinity</topic><topic>Biological and medical sciences</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Estrogens - immunology</topic><topic>Estrogens - urine</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Mammalian female genital system</topic><topic>Morphology. Physiology</topic><topic>Ovulation Detection</topic><topic>Radioimmunoassay</topic><topic>Reagent Kits, Diagnostic</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DE LAUZON, S</creatorcontrib><creatorcontrib>DESFOSSES, B</creatorcontrib><creatorcontrib>CHRISTEFF, N</creatorcontrib><creatorcontrib>HANQUEZ, C</creatorcontrib><creatorcontrib>CITTANOVA, N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DE LAUZON, S</au><au>DESFOSSES, B</au><au>CHRISTEFF, N</au><au>HANQUEZ, C</au><au>CITTANOVA, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A direct dot-enzyme immunoassay to detect human ovulation</atitle><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle><addtitle>J Steroid Biochem Mol Biol</addtitle><date>1992-04-01</date><risdate>1992</risdate><volume>42</volume><issue>2</issue><spage>223</spage><epage>228</epage><pages>223-228</pages><issn>0960-0760</issn><eissn>1879-1220</eissn><abstract>This paper describes an original dot-enzyme-linked immunosorbent assay (ELISA) for predicting ovulation in women, based on the detection of the pre-ovulatory estrogen peak in urine. A monoclonal anti-estrogen antibody is used which recognizes not only free estrogens but also some of their urinary metabolites (17-glucuro- and sulfo-conjugates) allowing a direct assay on early morning urines. Antigen is immobilized as a spot on a nitrocellulose membrane which is immersed in urine in the presence of this antibody. A peroxidase-labeled second antibody allows the detection of the first antibody bound to the membrane. Antigen and anti-estrogen antibody concentrations are chosen to obtain a maximal enzymatic coloration of spots corresponding to basal urinary estrogen levels and no coloration corresponding to the pre-ovulatory surge. Six menstrual cycles were studied, comparing dot-ELISA results with patterns of: (1) urinary estrogens measured by RIA either directly or after hydrolysis and extraction, and (2) basal body temperatures. The validity of the pre-ovulatory signal obtained and the requirements for an adaptation of this methodology to a reliable home kit are discussed.</abstract><cop>Oxford</cop><pub>Elsevier Science</pub><pmid>1567785</pmid><doi>10.1016/0960-0760(92)90031-D</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-0760 |
| ispartof | The Journal of steroid biochemistry and molecular biology, 1992-04, Vol.42 (2), p.223-228 |
| issn | 0960-0760 1879-1220 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antibodies, Monoclonal Antibody Affinity Biological and medical sciences Enzyme-Linked Immunosorbent Assay Estrogens - immunology Estrogens - urine Female Fundamental and applied biological sciences. Psychology Humans Hydrolysis Mammalian female genital system Morphology. Physiology Ovulation Detection Radioimmunoassay Reagent Kits, Diagnostic Vertebrates: reproduction |
| title | A direct dot-enzyme immunoassay to detect human ovulation |
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