Analysis of anti-keyhole limpet haemocyanin antibody in Brazilians supports its use for the diagnosis of acute schistosomiasis mansoni
Antibody (immunoglobulin (Ig) G) to the haemocyanin of the keyhole limpet (KLH) ( Megathura crenulata), which shares a well defined carbohydrate epitope with the surface of schistosomula of Schistosoma mansoni, was determined by enzyme-linked immunosorbent assay ( elisa) in the sera of Brazilians wi...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 1992, Vol.86 (1), p.53-56 |
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| creator | Alves-Brito, C.F. Simpson, A.J.G. Bahia-Oliveira, L.M.G. Rabello, A.L.T. Rocha, R.S. Lambertucci, J.R. Gazzinelli, G. Katz, N. Correa-Oliveira, R. |
| description | Antibody (immunoglobulin (Ig) G) to the haemocyanin of the keyhole limpet (KLH) (
Megathura crenulata), which shares a well defined carbohydrate epitope with the surface of schistosomula of
Schistosoma mansoni, was determined by enzyme-linked immunosorbent assay (
elisa) in the sera of Brazilians with acute schistosomiasis. Of 53 such individuals tested, 51 had a level of KLH reactivity in excess of the mean +2 standard deviations of that exhibited by chronically infected individuals. This difference in reactivity allowed the acute cases to be readily identified by visual inspection of
elisa plates. The levels of IgG in patients with hepatointestinal and hepatosplenic schistosomiasis, as well as in non-infected, seropositive residents of endemic areas and infected children from endemic areas, were not statistically different from those of intestinal patients. Significant levels of anti-KLH IgG were not detected in patients with leishmaniasis, Chagas disease, ancylostomiasis or ascariasis. The results support the use of KLH as a means of rapidly and easily identifying individuals with acute schistosomiasis. |
| doi_str_mv | 10.1016/0035-9203(92)90439-J |
| format | Article |
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Megathura crenulata), which shares a well defined carbohydrate epitope with the surface of schistosomula of
Schistosoma mansoni, was determined by enzyme-linked immunosorbent assay (
elisa) in the sera of Brazilians with acute schistosomiasis. Of 53 such individuals tested, 51 had a level of KLH reactivity in excess of the mean +2 standard deviations of that exhibited by chronically infected individuals. This difference in reactivity allowed the acute cases to be readily identified by visual inspection of
elisa plates. The levels of IgG in patients with hepatointestinal and hepatosplenic schistosomiasis, as well as in non-infected, seropositive residents of endemic areas and infected children from endemic areas, were not statistically different from those of intestinal patients. Significant levels of anti-KLH IgG were not detected in patients with leishmaniasis, Chagas disease, ancylostomiasis or ascariasis. The results support the use of KLH as a means of rapidly and easily identifying individuals with acute schistosomiasis.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1016/0035-9203(92)90439-J</identifier><identifier>PMID: 1566306</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Animals ; Antibodies, Helminth - immunology ; Brazil ; Child ; Hemocyanins - immunology ; Humans ; Immunoglobulin G - analysis ; Megathura crenulata ; Middle Aged ; Mollusca - immunology ; Schistosoma mansoni ; Schistosomiasis mansoni - diagnosis ; Schistosomiasis mansoni - immunology</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 1992, Vol.86 (1), p.53-56</ispartof><rights>1992</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-893f49e1c302965d49ce55094327435b287753dda9c7823ebd585f92091f21353</citedby><cites>FETCH-LOGICAL-c436t-893f49e1c302965d49ce55094327435b287753dda9c7823ebd585f92091f21353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4011,27905,27906,27907</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1566306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alves-Brito, C.F.</creatorcontrib><creatorcontrib>Simpson, A.J.G.</creatorcontrib><creatorcontrib>Bahia-Oliveira, L.M.G.</creatorcontrib><creatorcontrib>Rabello, A.L.T.</creatorcontrib><creatorcontrib>Rocha, R.S.</creatorcontrib><creatorcontrib>Lambertucci, J.R.</creatorcontrib><creatorcontrib>Gazzinelli, G.</creatorcontrib><creatorcontrib>Katz, N.</creatorcontrib><creatorcontrib>Correa-Oliveira, R.</creatorcontrib><title>Analysis of anti-keyhole limpet haemocyanin antibody in Brazilians supports its use for the diagnosis of acute schistosomiasis mansoni</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>Antibody (immunoglobulin (Ig) G) to the haemocyanin of the keyhole limpet (KLH) (
Megathura crenulata), which shares a well defined carbohydrate epitope with the surface of schistosomula of
Schistosoma mansoni, was determined by enzyme-linked immunosorbent assay (
elisa) in the sera of Brazilians with acute schistosomiasis. Of 53 such individuals tested, 51 had a level of KLH reactivity in excess of the mean +2 standard deviations of that exhibited by chronically infected individuals. This difference in reactivity allowed the acute cases to be readily identified by visual inspection of
elisa plates. The levels of IgG in patients with hepatointestinal and hepatosplenic schistosomiasis, as well as in non-infected, seropositive residents of endemic areas and infected children from endemic areas, were not statistically different from those of intestinal patients. Significant levels of anti-KLH IgG were not detected in patients with leishmaniasis, Chagas disease, ancylostomiasis or ascariasis. The results support the use of KLH as a means of rapidly and easily identifying individuals with acute schistosomiasis.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Antibodies, Helminth - immunology</subject><subject>Brazil</subject><subject>Child</subject><subject>Hemocyanins - immunology</subject><subject>Humans</subject><subject>Immunoglobulin G - analysis</subject><subject>Megathura crenulata</subject><subject>Middle Aged</subject><subject>Mollusca - immunology</subject><subject>Schistosoma mansoni</subject><subject>Schistosomiasis mansoni - diagnosis</subject><subject>Schistosomiasis mansoni - immunology</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAUhC0EKreFNwDJK0QXAf_EcbxBKhVQypWQoEgVG8vXOeGaJnGwHUR4AJ4bpyllBwv_SDNndDQfQo8oeUYJrZ4TwkWhGOFPFTtWpOSqOL-DNrSWdcEF4XfR5tZyHx3G-JUQJqhQB-iAiqripNqgXyeD6eboIvYtNkNyxRXMe98B7lw_QsJ7A723sxnccK3vfDPj_H8ZzE_XOTNEHKdx9CFF7PKZIuDWB5z2gBtnvgz-T7idEuBo9y4mH33vzCL0OcAP7gG615ouwsOb9wh9ev3q4vSs2L5_8_b0ZFvYklepqBVvSwXUcsJUJZpSWRCCqJIzWXKxY7WUgjeNUVbWjMOuEbVocwGKtoxywY_QkzV3DP7bBDHp3kULXWcG8FPUkilCZE3_a6RVqZgUS2K5Gm3wMQZo9Rhcb8KsKdELJ71A0AuEfOlrTvo8jz2-yZ92PTR_h1YwWS9WPbcFP25lE650JbkU-uzys6aXF_Tdxy3RH7L_xeqH3N53B0FH62Cw0LgANunGu38v9Bt7vrKv</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Alves-Brito, C.F.</creator><creator>Simpson, A.J.G.</creator><creator>Bahia-Oliveira, L.M.G.</creator><creator>Rabello, A.L.T.</creator><creator>Rocha, R.S.</creator><creator>Lambertucci, J.R.</creator><creator>Gazzinelli, G.</creator><creator>Katz, N.</creator><creator>Correa-Oliveira, R.</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>Analysis of anti-keyhole limpet haemocyanin antibody in Brazilians supports its use for the diagnosis of acute schistosomiasis mansoni</title><author>Alves-Brito, C.F. ; Simpson, A.J.G. ; Bahia-Oliveira, L.M.G. ; Rabello, A.L.T. ; Rocha, R.S. ; Lambertucci, J.R. ; Gazzinelli, G. ; Katz, N. ; Correa-Oliveira, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-893f49e1c302965d49ce55094327435b287753dda9c7823ebd585f92091f21353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Antibodies, Helminth - immunology</topic><topic>Brazil</topic><topic>Child</topic><topic>Hemocyanins - immunology</topic><topic>Humans</topic><topic>Immunoglobulin G - analysis</topic><topic>Megathura crenulata</topic><topic>Middle Aged</topic><topic>Mollusca - immunology</topic><topic>Schistosoma mansoni</topic><topic>Schistosomiasis mansoni - diagnosis</topic><topic>Schistosomiasis mansoni - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alves-Brito, C.F.</creatorcontrib><creatorcontrib>Simpson, A.J.G.</creatorcontrib><creatorcontrib>Bahia-Oliveira, L.M.G.</creatorcontrib><creatorcontrib>Rabello, A.L.T.</creatorcontrib><creatorcontrib>Rocha, R.S.</creatorcontrib><creatorcontrib>Lambertucci, J.R.</creatorcontrib><creatorcontrib>Gazzinelli, G.</creatorcontrib><creatorcontrib>Katz, N.</creatorcontrib><creatorcontrib>Correa-Oliveira, R.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alves-Brito, C.F.</au><au>Simpson, A.J.G.</au><au>Bahia-Oliveira, L.M.G.</au><au>Rabello, A.L.T.</au><au>Rocha, R.S.</au><au>Lambertucci, J.R.</au><au>Gazzinelli, G.</au><au>Katz, N.</au><au>Correa-Oliveira, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of anti-keyhole limpet haemocyanin antibody in Brazilians supports its use for the diagnosis of acute schistosomiasis mansoni</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>1992</date><risdate>1992</risdate><volume>86</volume><issue>1</issue><spage>53</spage><epage>56</epage><pages>53-56</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><abstract>Antibody (immunoglobulin (Ig) G) to the haemocyanin of the keyhole limpet (KLH) (
Megathura crenulata), which shares a well defined carbohydrate epitope with the surface of schistosomula of
Schistosoma mansoni, was determined by enzyme-linked immunosorbent assay (
elisa) in the sera of Brazilians with acute schistosomiasis. Of 53 such individuals tested, 51 had a level of KLH reactivity in excess of the mean +2 standard deviations of that exhibited by chronically infected individuals. This difference in reactivity allowed the acute cases to be readily identified by visual inspection of
elisa plates. The levels of IgG in patients with hepatointestinal and hepatosplenic schistosomiasis, as well as in non-infected, seropositive residents of endemic areas and infected children from endemic areas, were not statistically different from those of intestinal patients. Significant levels of anti-KLH IgG were not detected in patients with leishmaniasis, Chagas disease, ancylostomiasis or ascariasis. The results support the use of KLH as a means of rapidly and easily identifying individuals with acute schistosomiasis.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>1566306</pmid><doi>10.1016/0035-9203(92)90439-J</doi><tpages>4</tpages></addata></record> |
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| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 1992, Vol.86 (1), p.53-56 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adolescent Adult Aged Animals Antibodies, Helminth - immunology Brazil Child Hemocyanins - immunology Humans Immunoglobulin G - analysis Megathura crenulata Middle Aged Mollusca - immunology Schistosoma mansoni Schistosomiasis mansoni - diagnosis Schistosomiasis mansoni - immunology |
| title | Analysis of anti-keyhole limpet haemocyanin antibody in Brazilians supports its use for the diagnosis of acute schistosomiasis mansoni |
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