The reversible effect of glucose on the energy metabolism of Schistosoma mansoni cercariae and schistosomula
This study on isolated cercarial bodies demonstrates that the biological transformation from cercaria to schistosomulum and the biochemical transition from an aerobic to an anaerobic energy metabolism are separate processes, which are not necessarily linked. The metabolic transition depends on the e...
Gespeichert in:
| Veröffentlicht in: | Molecular and biochemical parasitology 1992-03, Vol.51 (1), p.73-79 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 79 |
|---|---|
| container_issue | 1 |
| container_start_page | 73 |
| container_title | Molecular and biochemical parasitology |
| container_volume | 51 |
| creator | Horemans, Anja M.C. Tielens, Aloysius G.M. van den Bergh, Simon G. |
| description | This study on isolated cercarial bodies demonstrates that the biological transformation from cercaria to schistosomulum and the biochemical transition from an aerobic to an anaerobic energy metabolism are separate processes, which are not necessarily linked. The metabolic transition depends on the external glucose concentration and is fully reversible. In the presence of only a tracer amount of [6-
14C]glucose, carbon dioxide was the major end product, but at higher glucose concentrations mainly lactate was formed. This effect could be demonstrated in cercarial bodies in water as well as in fully transformed schistosomula. In non-transformed cercariae a change towards a more anaerobic energy metabolism could be induced by an increase in the external glucose concentration, which demonstrated that the biochemical transition can occur in the absence of the biological transformation. Furthermore, the biological transformation can occur without a concomitant biochemical transition: in the presence of 5 mM glucose, lactate production by cercarial bodies during transformation was increased 50-fold, whereas in the presence of only a tracer amount of glucose the metabolic profile remained that of cercaraie. Also, in fully transformed schistosomula, this transition to a more anaerobic energy metabolism was induced by increased glucose concentrations, but at low glucose concentrations carbon dioxide was the major end product, as in cercariae. The effect of external glucose on the metabolism was fully reversible. After a high glucose concentration had induced a more anaerobic metabolism in cercariae in water, the metabolism returned to an aerobic one upon removal of the glucose. Likewise, the metabolism in schistosomula switched back and forth between anaerobic and aerobic patterns, following successive changes in the glucose concentration. The possible relevance of this exceptional glycolytic system in the metabolism of
Schistosoma mansoni is discussed. |
| doi_str_mv | 10.1016/0166-6851(92)90202-U |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72894294</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>016668519290202U</els_id><sourcerecordid>72894294</sourcerecordid><originalsourceid>FETCH-LOGICAL-c437t-6a5933e114bd8e1d27ef7adb05ca65ab47b1250e8542e4dd535174f344810543</originalsourceid><addsrcrecordid>eNp9kUGL1TAQx4Mo69vVb6CQg4geqkmaNO1FkMVVYcGDb89hmkx3I2mzZtqF_fa2vsfz5mGYw_z-w_Abxl5J8UEK2Xxcq6ma1sh3nXrfCSVUdfOE7WRrVdVp1T5luxPynJ0T_RJCGNs0Z-xMmsZILXYs7e-QF3zAQrFPyHEY0M88D_w2LT4T8jzxeWVwwnL7yEecoc8p0rgxP_1dpDlTHoGPMFGeIvdYPJQIyGEKnE7EkuAFezZAInx57Bdsf_Vlf_mtuv7x9fvl5-vK69rOVQOmq2uUUvehRRmUxcFC6IXx0Bjote2lMgJboxXqEExtpNVDrXUrhdH1BXt7WHtf8u8FaXZjJI8pwYR5IWdVu_rpNlAfQF8yUcHB3Zc4Qnl0UrjNsdsEuk2g65T769jdrLHXx_1LP2L4FzpIXedvjnMgD2koMPlIJ8woYU2tVuzTAcNVxUPE4shHnDyGWNYfuJDj_-_4Ayv7mRE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72894294</pqid></control><display><type>article</type><title>The reversible effect of glucose on the energy metabolism of Schistosoma mansoni cercariae and schistosomula</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Horemans, Anja M.C. ; Tielens, Aloysius G.M. ; van den Bergh, Simon G.</creator><creatorcontrib>Horemans, Anja M.C. ; Tielens, Aloysius G.M. ; van den Bergh, Simon G.</creatorcontrib><description>This study on isolated cercarial bodies demonstrates that the biological transformation from cercaria to schistosomulum and the biochemical transition from an aerobic to an anaerobic energy metabolism are separate processes, which are not necessarily linked. The metabolic transition depends on the external glucose concentration and is fully reversible. In the presence of only a tracer amount of [6-
14C]glucose, carbon dioxide was the major end product, but at higher glucose concentrations mainly lactate was formed. This effect could be demonstrated in cercarial bodies in water as well as in fully transformed schistosomula. In non-transformed cercariae a change towards a more anaerobic energy metabolism could be induced by an increase in the external glucose concentration, which demonstrated that the biochemical transition can occur in the absence of the biological transformation. Furthermore, the biological transformation can occur without a concomitant biochemical transition: in the presence of 5 mM glucose, lactate production by cercarial bodies during transformation was increased 50-fold, whereas in the presence of only a tracer amount of glucose the metabolic profile remained that of cercaraie. Also, in fully transformed schistosomula, this transition to a more anaerobic energy metabolism was induced by increased glucose concentrations, but at low glucose concentrations carbon dioxide was the major end product, as in cercariae. The effect of external glucose on the metabolism was fully reversible. After a high glucose concentration had induced a more anaerobic metabolism in cercariae in water, the metabolism returned to an aerobic one upon removal of the glucose. Likewise, the metabolism in schistosomula switched back and forth between anaerobic and aerobic patterns, following successive changes in the glucose concentration. The possible relevance of this exceptional glycolytic system in the metabolism of
Schistosoma mansoni is discussed.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/0166-6851(92)90202-U</identifier><identifier>PMID: 1565140</identifier><identifier>CODEN: MBIPDP</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Aerobic/anaerobic transition ; Anaerobiosis ; Animals ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; Energy Metabolism ; Fundamental and applied biological sciences. Psychology ; Glucose - metabolism ; Glycolysis ; Invertebrates ; Lactate production ; Lactates - metabolism ; Nemathelminthia. Plathelmintha ; Physiology. Development ; Schistosoma mansoni ; Schistosoma mansoni - growth & development ; Schistosoma mansoni - metabolism ; Transformation ; Water</subject><ispartof>Molecular and biochemical parasitology, 1992-03, Vol.51 (1), p.73-79</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-6a5933e114bd8e1d27ef7adb05ca65ab47b1250e8542e4dd535174f344810543</citedby><cites>FETCH-LOGICAL-c437t-6a5933e114bd8e1d27ef7adb05ca65ab47b1250e8542e4dd535174f344810543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0166-6851(92)90202-U$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5207532$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1565140$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Horemans, Anja M.C.</creatorcontrib><creatorcontrib>Tielens, Aloysius G.M.</creatorcontrib><creatorcontrib>van den Bergh, Simon G.</creatorcontrib><title>The reversible effect of glucose on the energy metabolism of Schistosoma mansoni cercariae and schistosomula</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>This study on isolated cercarial bodies demonstrates that the biological transformation from cercaria to schistosomulum and the biochemical transition from an aerobic to an anaerobic energy metabolism are separate processes, which are not necessarily linked. The metabolic transition depends on the external glucose concentration and is fully reversible. In the presence of only a tracer amount of [6-
14C]glucose, carbon dioxide was the major end product, but at higher glucose concentrations mainly lactate was formed. This effect could be demonstrated in cercarial bodies in water as well as in fully transformed schistosomula. In non-transformed cercariae a change towards a more anaerobic energy metabolism could be induced by an increase in the external glucose concentration, which demonstrated that the biochemical transition can occur in the absence of the biological transformation. Furthermore, the biological transformation can occur without a concomitant biochemical transition: in the presence of 5 mM glucose, lactate production by cercarial bodies during transformation was increased 50-fold, whereas in the presence of only a tracer amount of glucose the metabolic profile remained that of cercaraie. Also, in fully transformed schistosomula, this transition to a more anaerobic energy metabolism was induced by increased glucose concentrations, but at low glucose concentrations carbon dioxide was the major end product, as in cercariae. The effect of external glucose on the metabolism was fully reversible. After a high glucose concentration had induced a more anaerobic metabolism in cercariae in water, the metabolism returned to an aerobic one upon removal of the glucose. Likewise, the metabolism in schistosomula switched back and forth between anaerobic and aerobic patterns, following successive changes in the glucose concentration. The possible relevance of this exceptional glycolytic system in the metabolism of
Schistosoma mansoni is discussed.</description><subject>Aerobic/anaerobic transition</subject><subject>Anaerobiosis</subject><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>Energy Metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>Glycolysis</subject><subject>Invertebrates</subject><subject>Lactate production</subject><subject>Lactates - metabolism</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>Physiology. Development</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - growth & development</subject><subject>Schistosoma mansoni - metabolism</subject><subject>Transformation</subject><subject>Water</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUGL1TAQx4Mo69vVb6CQg4geqkmaNO1FkMVVYcGDb89hmkx3I2mzZtqF_fa2vsfz5mGYw_z-w_Abxl5J8UEK2Xxcq6ma1sh3nXrfCSVUdfOE7WRrVdVp1T5luxPynJ0T_RJCGNs0Z-xMmsZILXYs7e-QF3zAQrFPyHEY0M88D_w2LT4T8jzxeWVwwnL7yEecoc8p0rgxP_1dpDlTHoGPMFGeIvdYPJQIyGEKnE7EkuAFezZAInx57Bdsf_Vlf_mtuv7x9fvl5-vK69rOVQOmq2uUUvehRRmUxcFC6IXx0Bjote2lMgJboxXqEExtpNVDrXUrhdH1BXt7WHtf8u8FaXZjJI8pwYR5IWdVu_rpNlAfQF8yUcHB3Zc4Qnl0UrjNsdsEuk2g65T769jdrLHXx_1LP2L4FzpIXedvjnMgD2koMPlIJ8woYU2tVuzTAcNVxUPE4shHnDyGWNYfuJDj_-_4Ayv7mRE</recordid><startdate>19920301</startdate><enddate>19920301</enddate><creator>Horemans, Anja M.C.</creator><creator>Tielens, Aloysius G.M.</creator><creator>van den Bergh, Simon G.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920301</creationdate><title>The reversible effect of glucose on the energy metabolism of Schistosoma mansoni cercariae and schistosomula</title><author>Horemans, Anja M.C. ; Tielens, Aloysius G.M. ; van den Bergh, Simon G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-6a5933e114bd8e1d27ef7adb05ca65ab47b1250e8542e4dd535174f344810543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Aerobic/anaerobic transition</topic><topic>Anaerobiosis</topic><topic>Animals</topic><topic>Biochemistry. Physiology. Immunology. Molecular biology</topic><topic>Biological and medical sciences</topic><topic>Energy Metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>Glycolysis</topic><topic>Invertebrates</topic><topic>Lactate production</topic><topic>Lactates - metabolism</topic><topic>Nemathelminthia. Plathelmintha</topic><topic>Physiology. Development</topic><topic>Schistosoma mansoni</topic><topic>Schistosoma mansoni - growth & development</topic><topic>Schistosoma mansoni - metabolism</topic><topic>Transformation</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Horemans, Anja M.C.</creatorcontrib><creatorcontrib>Tielens, Aloysius G.M.</creatorcontrib><creatorcontrib>van den Bergh, Simon G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Horemans, Anja M.C.</au><au>Tielens, Aloysius G.M.</au><au>van den Bergh, Simon G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The reversible effect of glucose on the energy metabolism of Schistosoma mansoni cercariae and schistosomula</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1992-03-01</date><risdate>1992</risdate><volume>51</volume><issue>1</issue><spage>73</spage><epage>79</epage><pages>73-79</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><coden>MBIPDP</coden><abstract>This study on isolated cercarial bodies demonstrates that the biological transformation from cercaria to schistosomulum and the biochemical transition from an aerobic to an anaerobic energy metabolism are separate processes, which are not necessarily linked. The metabolic transition depends on the external glucose concentration and is fully reversible. In the presence of only a tracer amount of [6-
14C]glucose, carbon dioxide was the major end product, but at higher glucose concentrations mainly lactate was formed. This effect could be demonstrated in cercarial bodies in water as well as in fully transformed schistosomula. In non-transformed cercariae a change towards a more anaerobic energy metabolism could be induced by an increase in the external glucose concentration, which demonstrated that the biochemical transition can occur in the absence of the biological transformation. Furthermore, the biological transformation can occur without a concomitant biochemical transition: in the presence of 5 mM glucose, lactate production by cercarial bodies during transformation was increased 50-fold, whereas in the presence of only a tracer amount of glucose the metabolic profile remained that of cercaraie. Also, in fully transformed schistosomula, this transition to a more anaerobic energy metabolism was induced by increased glucose concentrations, but at low glucose concentrations carbon dioxide was the major end product, as in cercariae. The effect of external glucose on the metabolism was fully reversible. After a high glucose concentration had induced a more anaerobic metabolism in cercariae in water, the metabolism returned to an aerobic one upon removal of the glucose. Likewise, the metabolism in schistosomula switched back and forth between anaerobic and aerobic patterns, following successive changes in the glucose concentration. The possible relevance of this exceptional glycolytic system in the metabolism of
Schistosoma mansoni is discussed.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>1565140</pmid><doi>10.1016/0166-6851(92)90202-U</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-6851 |
| ispartof | Molecular and biochemical parasitology, 1992-03, Vol.51 (1), p.73-79 |
| issn | 0166-6851 1872-9428 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72894294 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Aerobic/anaerobic transition Anaerobiosis Animals Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Energy Metabolism Fundamental and applied biological sciences. Psychology Glucose - metabolism Glycolysis Invertebrates Lactate production Lactates - metabolism Nemathelminthia. Plathelmintha Physiology. Development Schistosoma mansoni Schistosoma mansoni - growth & development Schistosoma mansoni - metabolism Transformation Water |
| title | The reversible effect of glucose on the energy metabolism of Schistosoma mansoni cercariae and schistosomula |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T13%3A27%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20reversible%20effect%20of%20glucose%20on%20the%20energy%20metabolism%20of%20Schistosoma%20mansoni%20cercariae%20and%20schistosomula&rft.jtitle=Molecular%20and%20biochemical%20parasitology&rft.au=Horemans,%20Anja%20M.C.&rft.date=1992-03-01&rft.volume=51&rft.issue=1&rft.spage=73&rft.epage=79&rft.pages=73-79&rft.issn=0166-6851&rft.eissn=1872-9428&rft.coden=MBIPDP&rft_id=info:doi/10.1016/0166-6851(92)90202-U&rft_dat=%3Cproquest_cross%3E72894294%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72894294&rft_id=info:pmid/1565140&rft_els_id=016668519290202U&rfr_iscdi=true |