Isolation of cold agglutinins in Eperythrozoon suis-infected pigs

A simple and reliable procedure is described for isolating the cold agglutinins associated with Eperythrozoon suis-infection in swine. After initiating microagglutination by cooling the blood samples, the erythrocytes were separated by density-gradient centrifugation using Ficoll-Paque®, resuspended...

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Veröffentlicht in:	Veterinary immunology and immunopathology 1992-02, Vol.31 (1), p.195-201
Hauptverfasser:	Schmidt, P., Kaspers, B., Jüngling, A., Heinritzi, K., Lösch, U.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agglutination - immunology AGGLUTININE AGGLUTININS Agglutinins - isolation & purification AGLUTININAS Animal bacterial diseases Animals Bacterial diseases Biological and medical sciences CENTRIFUGACION CENTRIFUGATION Centrifugation, Density Gradient - veterinary CENTRIFUGING CERDO Electrophoresis, Polyacrylamide Gel - veterinary EPERYTHROZOON Immunoblotting - veterinary Immunodiffusion - veterinary IMMUNODIFFUSION TESTS Immunoglobulin M - analysis Infectious diseases Medical sciences METHODE METHODS METODOS Mycoplasma - immunology Mycoplasma Infections - immunology Mycoplasma Infections - veterinary PORCIN SWINE Swine Diseases - immunology TECHNIQUE D'IMMUNODIFFUSION TECNICAS DE INMUNODIFUSION
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