Isolation of cold agglutinins in Eperythrozoon suis-infected pigs
A simple and reliable procedure is described for isolating the cold agglutinins associated with Eperythrozoon suis-infection in swine. After initiating microagglutination by cooling the blood samples, the erythrocytes were separated by density-gradient centrifugation using Ficoll-Paque®, resuspended...
Gespeichert in:
| Veröffentlicht in: | Veterinary immunology and immunopathology 1992-02, Vol.31 (1), p.195-201 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 201 |
|---|---|
| container_issue | 1 |
| container_start_page | 195 |
| container_title | Veterinary immunology and immunopathology |
| container_volume | 31 |
| creator | Schmidt, P. Kaspers, B. Jüngling, A. Heinritzi, K. Lösch, U. |
| description | A simple and reliable procedure is described for isolating the cold agglutinins associated with
Eperythrozoon suis-infection in swine. After initiating microagglutination by cooling the blood samples, the erythrocytes were separated by density-gradient centrifugation using Ficoll-Paque®, resuspended in RPMI-medium and warmed to 40°C. The cold agglutinins could be removed from the supernatant by a subsequent centrifugation. Double immunodiffusion, polyacrylamide gel electrophoresis and immunoblotting showed that the cold agglutinins isolated consisted of IgM antibodies exclusively. Their ability to induce agglutination in cooled erythrocytes from healthy pigs confirmed that they were genuine cold agglutinins. The method paves the way for more detailed investigation into the mechanisms of this agglutinating disease. |
| doi_str_mv | 10.1016/0165-2427(92)90097-A |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72891900</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>016524279290097A</els_id><sourcerecordid>72891900</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-69f24fcd8e81cb16014f384059a0030fbe78d1a198f8ebf57b7c9b8f9c130b433</originalsourceid><addsrcrecordid>eNp9kMFq3DAQhkVo2G7SvEBowYdSkoObGVleSZfAEpJ0YUkuyVnIsrRV8VpbyQ6kT19tvKS3HoY5_N8_DB8hnxG-I-DiKk9dUkb5haSXEkDycnlE5ih4VdK6Yh_I_B35SE5S-gUAtRRiRmZYc1gImJPlKoVODz70RXCFCV1b6M2mGwff-z4Vvi9udza-Dj9j-BMylEafSt87awbbFju_SZ_IsdNdsmeHfUqe726fbn6U68f71c1yXRrGcSgX0lHmTCusQNPgApC5SrD8kAaowDWWixY1SuGEbVzNG25kI5w0WEHDquqUfJvu7mL4Pdo0qK1Pxnad7m0Yk-JUSMwaMsgm0MSQUrRO7aLf6viqENTenNprUXstSlL1Zk4tc-3L4f7YbG37rzSpyvnXQ66T0Z2Lujc-vWM1BckQM3Y-YU4HpTcxIw9rSQE5pTm8nkKbRb14G1Uy3vbGtj5mo6oN_v9P_gUQVJGp</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72891900</pqid></control><display><type>article</type><title>Isolation of cold agglutinins in Eperythrozoon suis-infected pigs</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Schmidt, P. ; Kaspers, B. ; Jüngling, A. ; Heinritzi, K. ; Lösch, U.</creator><creatorcontrib>Schmidt, P. ; Kaspers, B. ; Jüngling, A. ; Heinritzi, K. ; Lösch, U.</creatorcontrib><description>A simple and reliable procedure is described for isolating the cold agglutinins associated with
Eperythrozoon suis-infection in swine. After initiating microagglutination by cooling the blood samples, the erythrocytes were separated by density-gradient centrifugation using Ficoll-Paque®, resuspended in RPMI-medium and warmed to 40°C. The cold agglutinins could be removed from the supernatant by a subsequent centrifugation. Double immunodiffusion, polyacrylamide gel electrophoresis and immunoblotting showed that the cold agglutinins isolated consisted of IgM antibodies exclusively. Their ability to induce agglutination in cooled erythrocytes from healthy pigs confirmed that they were genuine cold agglutinins. The method paves the way for more detailed investigation into the mechanisms of this agglutinating disease.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/0165-2427(92)90097-A</identifier><identifier>PMID: 1570680</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Agglutination - immunology ; AGGLUTININE ; AGGLUTININS ; Agglutinins - isolation & purification ; AGLUTININAS ; Animal bacterial diseases ; Animals ; Bacterial diseases ; Biological and medical sciences ; CENTRIFUGACION ; CENTRIFUGATION ; Centrifugation, Density Gradient - veterinary ; CENTRIFUGING ; CERDO ; Electrophoresis, Polyacrylamide Gel - veterinary ; EPERYTHROZOON ; Immunoblotting - veterinary ; Immunodiffusion - veterinary ; IMMUNODIFFUSION TESTS ; Immunoglobulin M - analysis ; Infectious diseases ; Medical sciences ; METHODE ; METHODS ; METODOS ; Mycoplasma - immunology ; Mycoplasma Infections - immunology ; Mycoplasma Infections - veterinary ; PORCIN ; SWINE ; Swine Diseases - immunology ; TECHNIQUE D'IMMUNODIFFUSION ; TECNICAS DE INMUNODIFUSION</subject><ispartof>Veterinary immunology and immunopathology, 1992-02, Vol.31 (1), p.195-201</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-69f24fcd8e81cb16014f384059a0030fbe78d1a198f8ebf57b7c9b8f9c130b433</citedby><cites>FETCH-LOGICAL-c471t-69f24fcd8e81cb16014f384059a0030fbe78d1a198f8ebf57b7c9b8f9c130b433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/016524279290097A$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5209411$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1570680$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schmidt, P.</creatorcontrib><creatorcontrib>Kaspers, B.</creatorcontrib><creatorcontrib>Jüngling, A.</creatorcontrib><creatorcontrib>Heinritzi, K.</creatorcontrib><creatorcontrib>Lösch, U.</creatorcontrib><title>Isolation of cold agglutinins in Eperythrozoon suis-infected pigs</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>A simple and reliable procedure is described for isolating the cold agglutinins associated with
Eperythrozoon suis-infection in swine. After initiating microagglutination by cooling the blood samples, the erythrocytes were separated by density-gradient centrifugation using Ficoll-Paque®, resuspended in RPMI-medium and warmed to 40°C. The cold agglutinins could be removed from the supernatant by a subsequent centrifugation. Double immunodiffusion, polyacrylamide gel electrophoresis and immunoblotting showed that the cold agglutinins isolated consisted of IgM antibodies exclusively. Their ability to induce agglutination in cooled erythrocytes from healthy pigs confirmed that they were genuine cold agglutinins. The method paves the way for more detailed investigation into the mechanisms of this agglutinating disease.</description><subject>Agglutination - immunology</subject><subject>AGGLUTININE</subject><subject>AGGLUTININS</subject><subject>Agglutinins - isolation & purification</subject><subject>AGLUTININAS</subject><subject>Animal bacterial diseases</subject><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>CENTRIFUGACION</subject><subject>CENTRIFUGATION</subject><subject>Centrifugation, Density Gradient - veterinary</subject><subject>CENTRIFUGING</subject><subject>CERDO</subject><subject>Electrophoresis, Polyacrylamide Gel - veterinary</subject><subject>EPERYTHROZOON</subject><subject>Immunoblotting - veterinary</subject><subject>Immunodiffusion - veterinary</subject><subject>IMMUNODIFFUSION TESTS</subject><subject>Immunoglobulin M - analysis</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>METHODE</subject><subject>METHODS</subject><subject>METODOS</subject><subject>Mycoplasma - immunology</subject><subject>Mycoplasma Infections - immunology</subject><subject>Mycoplasma Infections - veterinary</subject><subject>PORCIN</subject><subject>SWINE</subject><subject>Swine Diseases - immunology</subject><subject>TECHNIQUE D'IMMUNODIFFUSION</subject><subject>TECNICAS DE INMUNODIFUSION</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFq3DAQhkVo2G7SvEBowYdSkoObGVleSZfAEpJ0YUkuyVnIsrRV8VpbyQ6kT19tvKS3HoY5_N8_DB8hnxG-I-DiKk9dUkb5haSXEkDycnlE5ih4VdK6Yh_I_B35SE5S-gUAtRRiRmZYc1gImJPlKoVODz70RXCFCV1b6M2mGwff-z4Vvi9udza-Dj9j-BMylEafSt87awbbFju_SZ_IsdNdsmeHfUqe726fbn6U68f71c1yXRrGcSgX0lHmTCusQNPgApC5SrD8kAaowDWWixY1SuGEbVzNG25kI5w0WEHDquqUfJvu7mL4Pdo0qK1Pxnad7m0Yk-JUSMwaMsgm0MSQUrRO7aLf6viqENTenNprUXstSlL1Zk4tc-3L4f7YbG37rzSpyvnXQ66T0Z2Lujc-vWM1BckQM3Y-YU4HpTcxIw9rSQE5pTm8nkKbRb14G1Uy3vbGtj5mo6oN_v9P_gUQVJGp</recordid><startdate>19920215</startdate><enddate>19920215</enddate><creator>Schmidt, P.</creator><creator>Kaspers, B.</creator><creator>Jüngling, A.</creator><creator>Heinritzi, K.</creator><creator>Lösch, U.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920215</creationdate><title>Isolation of cold agglutinins in Eperythrozoon suis-infected pigs</title><author>Schmidt, P. ; Kaspers, B. ; Jüngling, A. ; Heinritzi, K. ; Lösch, U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-69f24fcd8e81cb16014f384059a0030fbe78d1a198f8ebf57b7c9b8f9c130b433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Agglutination - immunology</topic><topic>AGGLUTININE</topic><topic>AGGLUTININS</topic><topic>Agglutinins - isolation & purification</topic><topic>AGLUTININAS</topic><topic>Animal bacterial diseases</topic><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>CENTRIFUGACION</topic><topic>CENTRIFUGATION</topic><topic>Centrifugation, Density Gradient - veterinary</topic><topic>CENTRIFUGING</topic><topic>CERDO</topic><topic>Electrophoresis, Polyacrylamide Gel - veterinary</topic><topic>EPERYTHROZOON</topic><topic>Immunoblotting - veterinary</topic><topic>Immunodiffusion - veterinary</topic><topic>IMMUNODIFFUSION TESTS</topic><topic>Immunoglobulin M - analysis</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>METHODE</topic><topic>METHODS</topic><topic>METODOS</topic><topic>Mycoplasma - immunology</topic><topic>Mycoplasma Infections - immunology</topic><topic>Mycoplasma Infections - veterinary</topic><topic>PORCIN</topic><topic>SWINE</topic><topic>Swine Diseases - immunology</topic><topic>TECHNIQUE D'IMMUNODIFFUSION</topic><topic>TECNICAS DE INMUNODIFUSION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schmidt, P.</creatorcontrib><creatorcontrib>Kaspers, B.</creatorcontrib><creatorcontrib>Jüngling, A.</creatorcontrib><creatorcontrib>Heinritzi, K.</creatorcontrib><creatorcontrib>Lösch, U.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schmidt, P.</au><au>Kaspers, B.</au><au>Jüngling, A.</au><au>Heinritzi, K.</au><au>Lösch, U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of cold agglutinins in Eperythrozoon suis-infected pigs</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>1992-02-15</date><risdate>1992</risdate><volume>31</volume><issue>1</issue><spage>195</spage><epage>201</epage><pages>195-201</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>A simple and reliable procedure is described for isolating the cold agglutinins associated with
Eperythrozoon suis-infection in swine. After initiating microagglutination by cooling the blood samples, the erythrocytes were separated by density-gradient centrifugation using Ficoll-Paque®, resuspended in RPMI-medium and warmed to 40°C. The cold agglutinins could be removed from the supernatant by a subsequent centrifugation. Double immunodiffusion, polyacrylamide gel electrophoresis and immunoblotting showed that the cold agglutinins isolated consisted of IgM antibodies exclusively. Their ability to induce agglutination in cooled erythrocytes from healthy pigs confirmed that they were genuine cold agglutinins. The method paves the way for more detailed investigation into the mechanisms of this agglutinating disease.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1570680</pmid><doi>10.1016/0165-2427(92)90097-A</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary immunology and immunopathology, 1992-02, Vol.31 (1), p.195-201 |
| issn | 0165-2427 1873-2534 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72891900 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agglutination - immunology AGGLUTININE AGGLUTININS Agglutinins - isolation & purification AGLUTININAS Animal bacterial diseases Animals Bacterial diseases Biological and medical sciences CENTRIFUGACION CENTRIFUGATION Centrifugation, Density Gradient - veterinary CENTRIFUGING CERDO Electrophoresis, Polyacrylamide Gel - veterinary EPERYTHROZOON Immunoblotting - veterinary Immunodiffusion - veterinary IMMUNODIFFUSION TESTS Immunoglobulin M - analysis Infectious diseases Medical sciences METHODE METHODS METODOS Mycoplasma - immunology Mycoplasma Infections - immunology Mycoplasma Infections - veterinary PORCIN SWINE Swine Diseases - immunology TECHNIQUE D'IMMUNODIFFUSION TECNICAS DE INMUNODIFUSION |
| title | Isolation of cold agglutinins in Eperythrozoon suis-infected pigs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T08%3A36%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20of%20cold%20agglutinins%20in%20Eperythrozoon%20suis-infected%20pigs&rft.jtitle=Veterinary%20immunology%20and%20immunopathology&rft.au=Schmidt,%20P.&rft.date=1992-02-15&rft.volume=31&rft.issue=1&rft.spage=195&rft.epage=201&rft.pages=195-201&rft.issn=0165-2427&rft.eissn=1873-2534&rft_id=info:doi/10.1016/0165-2427(92)90097-A&rft_dat=%3Cproquest_cross%3E72891900%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72891900&rft_id=info:pmid/1570680&rft_els_id=016524279290097A&rfr_iscdi=true |