Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle
Several studies have shown that both quiescent and proliferating somatic donor cells can be fully reprogrammed after nuclear transfer (NT) and result in viable offspring. So far, however, no comparative study has conclusively demonstrated the relative importance of donor cell cycle stage on nuclear...
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| Veröffentlicht in: | Theriogenology 2003, Vol.59 (1), p.45-59 |
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| container_title | Theriogenology |
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| creator | Wells, D.N Laible, G Tucker, F.C Miller, A.L Oliver, J.E Xiang, T Forsyth, J.T Berg, M.C Cockrem, K L’Huillier, P.J Tervit, H.R Oback, B |
| description | Several studies have shown that both quiescent and proliferating somatic donor cells can be fully reprogrammed after nuclear transfer (NT) and result in viable offspring. So far, however, no comparative study has conclusively demonstrated the relative importance of donor cell cycle stage on nuclear cloning efficiency. Here, we compare two different types of bovine fetal fibroblasts (BFFs) that were synchronized in G
0, G
1, and different phases within G
1. We show that for non-transgenic (non-TG) fibroblasts, serum starvation into G
0 results in a significantly higher percentage of viable calves at term than synchronization in early G
1 or late G
1. For transgenic fibroblasts, however, cells selected in G
1 show significantly higher development to calves at term and higher post-natal survival to weaning than cells in G
0. This suggests that it may be necessary to coordinate donor cell type and cell cycle stage to maximize overall cloning efficiency. |
| doi_str_mv | 10.1016/S0093-691X(02)01273-6 |
| format | Article |
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0, G
1, and different phases within G
1. We show that for non-transgenic (non-TG) fibroblasts, serum starvation into G
0 results in a significantly higher percentage of viable calves at term than synchronization in early G
1 or late G
1. For transgenic fibroblasts, however, cells selected in G
1 show significantly higher development to calves at term and higher post-natal survival to weaning than cells in G
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0, G
1, and different phases within G
1. We show that for non-transgenic (non-TG) fibroblasts, serum starvation into G
0 results in a significantly higher percentage of viable calves at term than synchronization in early G
1 or late G
1. For transgenic fibroblasts, however, cells selected in G
1 show significantly higher development to calves at term and higher post-natal survival to weaning than cells in G
0. This suggests that it may be necessary to coordinate donor cell type and cell cycle stage to maximize overall cloning efficiency.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cattle - embryology</subject><subject>Cattle - genetics</subject><subject>Cell Cycle</subject><subject>Cell type</subject><subject>Cloning</subject><subject>Cloning, Organism</subject><subject>Embryo Transfer - veterinary</subject><subject>Embryonic and Fetal Development</subject><subject>Female</subject><subject>Fibroblasts - ultrastructure</subject><subject>G1 Phase</subject><subject>G2 Phase</subject><subject>Mitosis</subject><subject>Nuclear transfer</subject><subject>Nuclear Transfer Techniques</subject><subject>Pregnancy</subject><subject>Resting Phase, Cell Cycle</subject><subject>Transgenic</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlqHDEQQEVIiMdOPsFGpxAfOi6pu9WtkzFDNjDkEAdyE1pKg0yPNJY0DvP36VlwjjkVVbzaHiGXDD4xYOLmJ4BsGyHZ74_Ar4HxYc5ekQUbB9m0vGWvyeIFOSPnpTwCQCsEe0vOGO-kBDYsyGqZUnYh6hpSpAbrH8RIXYopU4vTROtug1RHd8zszk5IS9UrpGG9yekZC43buahnfkoxxBVF74MNGO2OhkitrnXCd-SN11PB96d4QX59-fyw_Nbc__j6fXl339hukLVxKJl21qB3aIwdAMYOWAfGez_iaBj2ghs-Cg5u1EJL3YreC6P7Thjb9-0F-XCcO9_2tMVS1TqU_ek6YtoWNfBRghj5DPZH0OZUSkavNjmsdd4pBmpvWB0Mq70-BVwdDCsx912dFmzNGt2_rpPSGbg9Aji_-Rwwq3KQgS5ktFW5FP6z4i8RUY3i</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Wells, D.N</creator><creator>Laible, G</creator><creator>Tucker, F.C</creator><creator>Miller, A.L</creator><creator>Oliver, J.E</creator><creator>Xiang, T</creator><creator>Forsyth, J.T</creator><creator>Berg, M.C</creator><creator>Cockrem, K</creator><creator>L’Huillier, P.J</creator><creator>Tervit, H.R</creator><creator>Oback, B</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle</title><author>Wells, D.N ; Laible, G ; Tucker, F.C ; Miller, A.L ; Oliver, J.E ; Xiang, T ; Forsyth, J.T ; Berg, M.C ; Cockrem, K ; L’Huillier, P.J ; Tervit, H.R ; Oback, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-de91adcbefdebbc700840140bfff8e8b1e562b28620d8a6a9a365f6ba546bc553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Cattle - embryology</topic><topic>Cattle - genetics</topic><topic>Cell Cycle</topic><topic>Cell type</topic><topic>Cloning</topic><topic>Cloning, Organism</topic><topic>Embryo Transfer - veterinary</topic><topic>Embryonic and Fetal Development</topic><topic>Female</topic><topic>Fibroblasts - ultrastructure</topic><topic>G1 Phase</topic><topic>G2 Phase</topic><topic>Mitosis</topic><topic>Nuclear transfer</topic><topic>Nuclear Transfer Techniques</topic><topic>Pregnancy</topic><topic>Resting Phase, Cell Cycle</topic><topic>Transgenic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wells, D.N</creatorcontrib><creatorcontrib>Laible, G</creatorcontrib><creatorcontrib>Tucker, F.C</creatorcontrib><creatorcontrib>Miller, A.L</creatorcontrib><creatorcontrib>Oliver, J.E</creatorcontrib><creatorcontrib>Xiang, T</creatorcontrib><creatorcontrib>Forsyth, J.T</creatorcontrib><creatorcontrib>Berg, M.C</creatorcontrib><creatorcontrib>Cockrem, K</creatorcontrib><creatorcontrib>L’Huillier, P.J</creatorcontrib><creatorcontrib>Tervit, H.R</creatorcontrib><creatorcontrib>Oback, B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wells, D.N</au><au>Laible, G</au><au>Tucker, F.C</au><au>Miller, A.L</au><au>Oliver, J.E</au><au>Xiang, T</au><au>Forsyth, J.T</au><au>Berg, M.C</au><au>Cockrem, K</au><au>L’Huillier, P.J</au><au>Tervit, H.R</au><au>Oback, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2003</date><risdate>2003</risdate><volume>59</volume><issue>1</issue><spage>45</spage><epage>59</epage><pages>45-59</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>Several studies have shown that both quiescent and proliferating somatic donor cells can be fully reprogrammed after nuclear transfer (NT) and result in viable offspring. So far, however, no comparative study has conclusively demonstrated the relative importance of donor cell cycle stage on nuclear cloning efficiency. Here, we compare two different types of bovine fetal fibroblasts (BFFs) that were synchronized in G
0, G
1, and different phases within G
1. We show that for non-transgenic (non-TG) fibroblasts, serum starvation into G
0 results in a significantly higher percentage of viable calves at term than synchronization in early G
1 or late G
1. For transgenic fibroblasts, however, cells selected in G
1 show significantly higher development to calves at term and higher post-natal survival to weaning than cells in G
0. This suggests that it may be necessary to coordinate donor cell type and cell cycle stage to maximize overall cloning efficiency.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12499017</pmid><doi>10.1016/S0093-691X(02)01273-6</doi><tpages>15</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Cattle Cattle - embryology Cattle - genetics Cell Cycle Cell type Cloning Cloning, Organism Embryo Transfer - veterinary Embryonic and Fetal Development Female Fibroblasts - ultrastructure G1 Phase G2 Phase Mitosis Nuclear transfer Nuclear Transfer Techniques Pregnancy Resting Phase, Cell Cycle Transgenic |
| title | Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle |
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