The Antigen-Antibody Reaction in Immunohistochemistry
The problems of major concern in immunohistochemical practice are discussed in the following order: (a) the mechanism of the Ag-Ab reaction in fixed tissue as opposed to the in vitro reaction; (b) the chemistry of fixation and its influence on the final result of the immunohistochemical reaction; (c...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 2003-01, Vol.51 (1), p.1-4 |
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| creator | Montero, Claudio |
| description | The problems of major concern in immunohistochemical practice are discussed in the following order: (a) the mechanism of the Ag-Ab reaction in fixed tissue as opposed to the in vitro reaction; (b) the chemistry of fixation and its influence on the final result of the immunohistochemical reaction; (c) the various procedures used for antigen retrieval in formaldehyde-fixed tissue; and (d) the consideration of the possible mechanism underlying heat-induced antigen retrieval. Suggestions for further work to attempt a clarification of the mechanism involved in the Ag-Ab reaction in immunohistochemistry resorting to existing histochemical methods for the demonstration of protein side groups are presented, together with some examples already published. |
| doi_str_mv | 10.1177/002215540305100101 |
| format | Article |
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| source | SAGE Complete A-Z List; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antigen-Antibody Complex Antigens - metabolism Binding Sites, Antibody Fixatives Formaldehyde Hot Temperature Humans Immunohistochemistry - methods Proteins - chemistry Proteins - metabolism Tissue Fixation |
| title | The Antigen-Antibody Reaction in Immunohistochemistry |
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