A simple and rapid radioimmunoassay for human interleukin-6

Studies were undertaken to develop a sensitive radioimmunoassay for human IL-6 using commercially available reagents. The assay utilized a polyclonal rabbit anti-IL-6 binding to iodinated IL-6; the reaction was carried out in solution and the immune complexes were precipitated using anti-rabbit IgG...

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Veröffentlicht in:	Journal of immunological methods 1992, Vol.148 (1), p.23-28
Hauptverfasser:	Peppard, Jane V., Rediske, John J., Koehler, Julie A., Koehne, Charles F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of the immune response. Humoral and cellular immunity Biological and medical sciences Bucladesine - immunology Cells, Cultured Dose-Response Relationship, Immunologic Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Interleukin-1 - immunology Interleukin-6 Interleukin-6 - analysis Lipopolysaccharides - immunology Lymphokines, interleukins ( function, expression) Monocyte, human Monocytes - metabolism Radioimmunoassay Radioimmunoassay - methods Reference Values Regulatory factors and their cellular receptors Sensitivity and Specificity
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