A simple and rapid radioimmunoassay for human interleukin-6
Studies were undertaken to develop a sensitive radioimmunoassay for human IL-6 using commercially available reagents. The assay utilized a polyclonal rabbit anti-IL-6 binding to iodinated IL-6; the reaction was carried out in solution and the immune complexes were precipitated using anti-rabbit IgG...
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| Veröffentlicht in: | Journal of immunological methods 1992, Vol.148 (1), p.23-28 |
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| creator | Peppard, Jane V. Rediske, John J. Koehler, Julie A. Koehne, Charles F. |
| description | Studies were undertaken to develop a sensitive radioimmunoassay for human IL-6 using commercially available reagents. The assay utilized a polyclonal rabbit anti-IL-6 binding to iodinated IL-6; the reaction was carried out in solution and the immune complexes were precipitated using anti-rabbit IgG coupled to magnetic particles. Using a format where sample IL-6 was added in advance of the radiolabelled IL-6, the working range of the assay was found to fall between 0.1 and 10 ng/ml (IC
50 around 1 ng/ml) for a 50 μl sample volume, and was run overnight. However, the assay could be completed in 2 h, using a direct competitive format when less sensitivity is required (working range 1.5–25 ng/ml, IC
50 12 ng/ml). The RIA correlated directly with a standard functional assay for IL-6 (proliferation of the mouse hybridoma B9.9) for both recombinant IL-6 and natural IL-6 from human monocytes. The total assay variance was less than 12% and no reactivity with interleukins-1–5 was found. Using the RIA, IL-6 produced in culture by human monocytes in response to various stimuli (LPS, IL-1, dibutyryl cAMP) was measured. |
| doi_str_mv | 10.1016/0022-1759(92)90154-L |
| format | Article |
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50 around 1 ng/ml) for a 50 μl sample volume, and was run overnight. However, the assay could be completed in 2 h, using a direct competitive format when less sensitivity is required (working range 1.5–25 ng/ml, IC
50 12 ng/ml). The RIA correlated directly with a standard functional assay for IL-6 (proliferation of the mouse hybridoma B9.9) for both recombinant IL-6 and natural IL-6 from human monocytes. The total assay variance was less than 12% and no reactivity with interleukins-1–5 was found. Using the RIA, IL-6 produced in culture by human monocytes in response to various stimuli (LPS, IL-1, dibutyryl cAMP) was measured.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(92)90154-L</identifier><identifier>PMID: 1314274</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Biological and medical sciences ; Bucladesine - immunology ; Cells, Cultured ; Dose-Response Relationship, Immunologic ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunobiology ; Interleukin-1 - immunology ; Interleukin-6 ; Interleukin-6 - analysis ; Lipopolysaccharides - immunology ; Lymphokines, interleukins ( function, expression) ; Monocyte, human ; Monocytes - metabolism ; Radioimmunoassay ; Radioimmunoassay - methods ; Reference Values ; Regulatory factors and their cellular receptors ; Sensitivity and Specificity</subject><ispartof>Journal of immunological methods, 1992, Vol.148 (1), p.23-28</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-2d794ae0c2189f11c937120584cc56669c704c5bbd2468cba2a7a0fa6e2457dd3</citedby><cites>FETCH-LOGICAL-c417t-2d794ae0c2189f11c937120584cc56669c704c5bbd2468cba2a7a0fa6e2457dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/002217599290154L$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5167843$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1314274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peppard, Jane V.</creatorcontrib><creatorcontrib>Rediske, John J.</creatorcontrib><creatorcontrib>Koehler, Julie A.</creatorcontrib><creatorcontrib>Koehne, Charles F.</creatorcontrib><title>A simple and rapid radioimmunoassay for human interleukin-6</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>Studies were undertaken to develop a sensitive radioimmunoassay for human IL-6 using commercially available reagents. The assay utilized a polyclonal rabbit anti-IL-6 binding to iodinated IL-6; the reaction was carried out in solution and the immune complexes were precipitated using anti-rabbit IgG coupled to magnetic particles. Using a format where sample IL-6 was added in advance of the radiolabelled IL-6, the working range of the assay was found to fall between 0.1 and 10 ng/ml (IC
50 around 1 ng/ml) for a 50 μl sample volume, and was run overnight. However, the assay could be completed in 2 h, using a direct competitive format when less sensitivity is required (working range 1.5–25 ng/ml, IC
50 12 ng/ml). The RIA correlated directly with a standard functional assay for IL-6 (proliferation of the mouse hybridoma B9.9) for both recombinant IL-6 and natural IL-6 from human monocytes. The total assay variance was less than 12% and no reactivity with interleukins-1–5 was found. Using the RIA, IL-6 produced in culture by human monocytes in response to various stimuli (LPS, IL-1, dibutyryl cAMP) was measured.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Biological and medical sciences</subject><subject>Bucladesine - immunology</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Interleukin-1 - immunology</subject><subject>Interleukin-6</subject><subject>Interleukin-6 - analysis</subject><subject>Lipopolysaccharides - immunology</subject><subject>Lymphokines, interleukins ( function, expression)</subject><subject>Monocyte, human</subject><subject>Monocytes - metabolism</subject><subject>Radioimmunoassay</subject><subject>Radioimmunoassay - methods</subject><subject>Reference Values</subject><subject>Regulatory factors and their cellular receptors</subject><subject>Sensitivity and Specificity</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAQx4Mo6_r4Bgo9iOihmknzaBCEZfEFBS96DtkkxWgfa9IK--1t3WW96RxmDv_fDMMPoRPAV4CBX2NMSAqCyQtJLiUGRtNiB00hFyQVErNdNN0i--ggxneMMWCOJ2gCGVAi6BTdzJLo62XlEt3YJOilH7v1ra_rvml1jHqVlG1I3vpaN4lvOhcq13_4JuVHaK_UVXTHm3mIXu_vXuaPafH88DSfFamhILqUWCGpdtgQyGUJYGQmgGCWU2MY51wagalhi4UllOdmoYkWGpeaO0KZsDY7ROfru8vQfvYudqr20biq0o1r-6gEyYei-b8gcJIRztgA0jVoQhtjcKVaBl_rsFKA1ShXjebUaE5Jon7kqmJYO93c7xe1s79La5tDfrbJdTS6KoNujI9bjAEXOc0G7HaNuUHal3dBReNdY5z1wZlO2db__cc3dlST8A</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Peppard, Jane V.</creator><creator>Rediske, John J.</creator><creator>Koehler, Julie A.</creator><creator>Koehne, Charles F.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>A simple and rapid radioimmunoassay for human interleukin-6</title><author>Peppard, Jane V. ; Rediske, John J. ; Koehler, Julie A. ; Koehne, Charles F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-2d794ae0c2189f11c937120584cc56669c704c5bbd2468cba2a7a0fa6e2457dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Biological and medical sciences</topic><topic>Bucladesine - immunology</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Interleukin-1 - immunology</topic><topic>Interleukin-6</topic><topic>Interleukin-6 - analysis</topic><topic>Lipopolysaccharides - immunology</topic><topic>Lymphokines, interleukins ( function, expression)</topic><topic>Monocyte, human</topic><topic>Monocytes - metabolism</topic><topic>Radioimmunoassay</topic><topic>Radioimmunoassay - methods</topic><topic>Reference Values</topic><topic>Regulatory factors and their cellular receptors</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peppard, Jane V.</creatorcontrib><creatorcontrib>Rediske, John J.</creatorcontrib><creatorcontrib>Koehler, Julie A.</creatorcontrib><creatorcontrib>Koehne, Charles F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peppard, Jane V.</au><au>Rediske, John J.</au><au>Koehler, Julie A.</au><au>Koehne, Charles F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple and rapid radioimmunoassay for human interleukin-6</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1992</date><risdate>1992</risdate><volume>148</volume><issue>1</issue><spage>23</spage><epage>28</epage><pages>23-28</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>Studies were undertaken to develop a sensitive radioimmunoassay for human IL-6 using commercially available reagents. The assay utilized a polyclonal rabbit anti-IL-6 binding to iodinated IL-6; the reaction was carried out in solution and the immune complexes were precipitated using anti-rabbit IgG coupled to magnetic particles. Using a format where sample IL-6 was added in advance of the radiolabelled IL-6, the working range of the assay was found to fall between 0.1 and 10 ng/ml (IC
50 around 1 ng/ml) for a 50 μl sample volume, and was run overnight. However, the assay could be completed in 2 h, using a direct competitive format when less sensitivity is required (working range 1.5–25 ng/ml, IC
50 12 ng/ml). The RIA correlated directly with a standard functional assay for IL-6 (proliferation of the mouse hybridoma B9.9) for both recombinant IL-6 and natural IL-6 from human monocytes. The total assay variance was less than 12% and no reactivity with interleukins-1–5 was found. Using the RIA, IL-6 produced in culture by human monocytes in response to various stimuli (LPS, IL-1, dibutyryl cAMP) was measured.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1314274</pmid><doi>10.1016/0022-1759(92)90154-L</doi><tpages>6</tpages></addata></record> |
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| subjects | Analysis of the immune response. Humoral and cellular immunity Biological and medical sciences Bucladesine - immunology Cells, Cultured Dose-Response Relationship, Immunologic Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Interleukin-1 - immunology Interleukin-6 Interleukin-6 - analysis Lipopolysaccharides - immunology Lymphokines, interleukins ( function, expression) Monocyte, human Monocytes - metabolism Radioimmunoassay Radioimmunoassay - methods Reference Values Regulatory factors and their cellular receptors Sensitivity and Specificity |
| title | A simple and rapid radioimmunoassay for human interleukin-6 |
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