Secretion of a collectin-like protein in tunicates is enhanced during inflammatory responses
The sub-cellular and humoral concentrations of a collectin-like protein from the solitary tunicate, Styela plicata, were measured after in vivo challenge with the inflammatory agent, zymosan. Tunicates were injected with zymosan before hemocytes and serum were harvested, subjected to western blottin...
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| Veröffentlicht in: | Developmental and comparative immunology 2003, Vol.27 (1), p.3-9 |
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| creator | Green, Peter L Nair, Sham V Raftos, David A |
| description | The sub-cellular and humoral concentrations of a collectin-like protein from the solitary tunicate,
Styela plicata, were measured after in vivo challenge with the inflammatory agent, zymosan. Tunicates were injected with zymosan before hemocytes and serum were harvested, subjected to western blotting and immunostained with an anti-
S. plicata collectin antibody to determine the relative titers of collectin-like proteins. Concentrations of the predominant 43
kDa collectin polypeptide were found to decrease in hemocytes immediately after zymosan injection, before rising to levels that were six times higher than controls within 96
h. Similarly, immunohistochemistry showed that the frequency of collectin-positive hemocytes in the circulating hemolymph increased significantly within 96
h of injection. Levels of the 43
kDa polypeptide in serum mirrored those of hemocytes. Humoral collectin concentrations decreased immediately after zymosan injection before rising, within 96
h post-injection, to levels three times higher than controls. This response to an inflammatory stimulus resembles that of mammalian collectins like mannose-binding lectin. The data suggest that, like its mammalian counterparts, the tunicate collectin acts as an acute phase antigen recognition protein. |
| doi_str_mv | 10.1016/S0145-305X(02)00067-8 |
| format | Article |
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Styela plicata, were measured after in vivo challenge with the inflammatory agent, zymosan. Tunicates were injected with zymosan before hemocytes and serum were harvested, subjected to western blotting and immunostained with an anti-
S. plicata collectin antibody to determine the relative titers of collectin-like proteins. Concentrations of the predominant 43
kDa collectin polypeptide were found to decrease in hemocytes immediately after zymosan injection, before rising to levels that were six times higher than controls within 96
h. Similarly, immunohistochemistry showed that the frequency of collectin-positive hemocytes in the circulating hemolymph increased significantly within 96
h of injection. Levels of the 43
kDa polypeptide in serum mirrored those of hemocytes. Humoral collectin concentrations decreased immediately after zymosan injection before rising, within 96
h post-injection, to levels three times higher than controls. This response to an inflammatory stimulus resembles that of mammalian collectins like mannose-binding lectin. The data suggest that, like its mammalian counterparts, the tunicate collectin acts as an acute phase antigen recognition protein.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/S0145-305X(02)00067-8</identifier><identifier>PMID: 12477496</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Animals ; Ascidiacea ; Ascidian ; Collectin ; collectins ; Collectins - analysis ; Collectins - chemistry ; Collectins - metabolism ; Hemocytes - metabolism ; Hemolymph - immunology ; Hemolymph - metabolism ; Inflammation - chemically induced ; Inflammation - immunology ; Innate immunity ; Mannose-binding lectin ; Marine ; Molecular Weight ; Styela plicata ; Time Factors ; Tunicate ; Urochordata - immunology ; Urochordata - metabolism ; Zymosan</subject><ispartof>Developmental and comparative immunology, 2003, Vol.27 (1), p.3-9</ispartof><rights>2002 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-923b0b29e979779b57cdf8d45b341642b572e43875d9888fb5442e23c56a1c8e3</citedby><cites>FETCH-LOGICAL-c392t-923b0b29e979779b57cdf8d45b341642b572e43875d9888fb5442e23c56a1c8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0145-305X(02)00067-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,4023,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12477496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Green, Peter L</creatorcontrib><creatorcontrib>Nair, Sham V</creatorcontrib><creatorcontrib>Raftos, David A</creatorcontrib><title>Secretion of a collectin-like protein in tunicates is enhanced during inflammatory responses</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>The sub-cellular and humoral concentrations of a collectin-like protein from the solitary tunicate,
Styela plicata, were measured after in vivo challenge with the inflammatory agent, zymosan. Tunicates were injected with zymosan before hemocytes and serum were harvested, subjected to western blotting and immunostained with an anti-
S. plicata collectin antibody to determine the relative titers of collectin-like proteins. Concentrations of the predominant 43
kDa collectin polypeptide were found to decrease in hemocytes immediately after zymosan injection, before rising to levels that were six times higher than controls within 96
h. Similarly, immunohistochemistry showed that the frequency of collectin-positive hemocytes in the circulating hemolymph increased significantly within 96
h of injection. Levels of the 43
kDa polypeptide in serum mirrored those of hemocytes. Humoral collectin concentrations decreased immediately after zymosan injection before rising, within 96
h post-injection, to levels three times higher than controls. This response to an inflammatory stimulus resembles that of mammalian collectins like mannose-binding lectin. The data suggest that, like its mammalian counterparts, the tunicate collectin acts as an acute phase antigen recognition protein.</description><subject>Animals</subject><subject>Ascidiacea</subject><subject>Ascidian</subject><subject>Collectin</subject><subject>collectins</subject><subject>Collectins - analysis</subject><subject>Collectins - chemistry</subject><subject>Collectins - metabolism</subject><subject>Hemocytes - metabolism</subject><subject>Hemolymph - immunology</subject><subject>Hemolymph - metabolism</subject><subject>Inflammation - chemically induced</subject><subject>Inflammation - immunology</subject><subject>Innate immunity</subject><subject>Mannose-binding lectin</subject><subject>Marine</subject><subject>Molecular Weight</subject><subject>Styela plicata</subject><subject>Time Factors</subject><subject>Tunicate</subject><subject>Urochordata - immunology</subject><subject>Urochordata - metabolism</subject><subject>Zymosan</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLHTEUgEOx1Kv2J1SykrqYNskkk2Qlcqm2IHRhCy6EkMmc0ehMck1mCvffm_vALoUDB875zoMPoS-UfKOENt9vCeWiqom4-0rYOSGkkZX6gBZUSV0RovQBWrwhh-go56cCEUXJJ3RIGZeS62aB7m_BJZh8DDj22GIXhwHc5EM1-GfAqxQn8AGXmObgnZ0gY58xhEcbHHS4m5MPD6XfD3Yc7RTTGifIqxgy5BP0sbdDhs_7fIz-Xv34s_xZ3fy-_rW8vKlcrdlUaVa3pGUatNRS6lZI1_Wq46KtOW04KwUGvFZSdFop1beCcwasdqKx1Cmoj9HZbm9592WGPJnRZwfDYAPEORvJlCp76ndBqhrBCBUFFDvQpZhzgt6skh9tWhtKzMa_2fo3G7mGMLP1b1SZO90fmNsRuv9Te-EFuNgBUHz885BMdh42Kn0q3k0X_TsnXgEGkZV0</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Green, Peter L</creator><creator>Nair, Sham V</creator><creator>Raftos, David A</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Secretion of a collectin-like protein in tunicates is enhanced during inflammatory responses</title><author>Green, Peter L ; Nair, Sham V ; Raftos, David A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-923b0b29e979779b57cdf8d45b341642b572e43875d9888fb5442e23c56a1c8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Ascidiacea</topic><topic>Ascidian</topic><topic>Collectin</topic><topic>collectins</topic><topic>Collectins - analysis</topic><topic>Collectins - chemistry</topic><topic>Collectins - metabolism</topic><topic>Hemocytes - metabolism</topic><topic>Hemolymph - immunology</topic><topic>Hemolymph - metabolism</topic><topic>Inflammation - chemically induced</topic><topic>Inflammation - immunology</topic><topic>Innate immunity</topic><topic>Mannose-binding lectin</topic><topic>Marine</topic><topic>Molecular Weight</topic><topic>Styela plicata</topic><topic>Time Factors</topic><topic>Tunicate</topic><topic>Urochordata - immunology</topic><topic>Urochordata - metabolism</topic><topic>Zymosan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Green, Peter L</creatorcontrib><creatorcontrib>Nair, Sham V</creatorcontrib><creatorcontrib>Raftos, David A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Green, Peter L</au><au>Nair, Sham V</au><au>Raftos, David A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Secretion of a collectin-like protein in tunicates is enhanced during inflammatory responses</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2003</date><risdate>2003</risdate><volume>27</volume><issue>1</issue><spage>3</spage><epage>9</epage><pages>3-9</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>The sub-cellular and humoral concentrations of a collectin-like protein from the solitary tunicate,
Styela plicata, were measured after in vivo challenge with the inflammatory agent, zymosan. Tunicates were injected with zymosan before hemocytes and serum were harvested, subjected to western blotting and immunostained with an anti-
S. plicata collectin antibody to determine the relative titers of collectin-like proteins. Concentrations of the predominant 43
kDa collectin polypeptide were found to decrease in hemocytes immediately after zymosan injection, before rising to levels that were six times higher than controls within 96
h. Similarly, immunohistochemistry showed that the frequency of collectin-positive hemocytes in the circulating hemolymph increased significantly within 96
h of injection. Levels of the 43
kDa polypeptide in serum mirrored those of hemocytes. Humoral collectin concentrations decreased immediately after zymosan injection before rising, within 96
h post-injection, to levels three times higher than controls. This response to an inflammatory stimulus resembles that of mammalian collectins like mannose-binding lectin. The data suggest that, like its mammalian counterparts, the tunicate collectin acts as an acute phase antigen recognition protein.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>12477496</pmid><doi>10.1016/S0145-305X(02)00067-8</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Ascidiacea Ascidian Collectin collectins Collectins - analysis Collectins - chemistry Collectins - metabolism Hemocytes - metabolism Hemolymph - immunology Hemolymph - metabolism Inflammation - chemically induced Inflammation - immunology Innate immunity Mannose-binding lectin Marine Molecular Weight Styela plicata Time Factors Tunicate Urochordata - immunology Urochordata - metabolism Zymosan |
| title | Secretion of a collectin-like protein in tunicates is enhanced during inflammatory responses |
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