Molecular characterization of XVSAP1, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker
The strategy of 'complementation by functional sufficiency' was used to isolate a cDNA designated XVSAP1 from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Sout...
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| Veröffentlicht in: | Journal of experimental botany 2003-01, Vol.54 (381), p.191-201 |
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| description | The strategy of 'complementation by functional sufficiency' was used to isolate a cDNA designated XVSAP1 from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low-temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana. Expression of XVSAP1 in Escherichia coli (srl::Tn10) conferred osmotic stress tolerance when the cells were grown in 1 M sorbitol. Analysis of gene expression using semi-quantitative RT-PCR indicated that XVSAP1 is induced by dehydration, salt stress (100 mM), both low (4 °C) and high temperature (42 °C) and high light treatment (1500 μmol m-2 s-1). These results suggest that XVSAP1 may have a significant role to play in the response of X. viscosa to abiotic stresses. |
| doi_str_mv | 10.1093/jxb/54.381.191 |
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Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low-temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana. Expression of XVSAP1 in Escherichia coli (srl::Tn10) conferred osmotic stress tolerance when the cells were grown in 1 M sorbitol. Analysis of gene expression using semi-quantitative RT-PCR indicated that XVSAP1 is induced by dehydration, salt stress (100 mM), both low (4 °C) and high temperature (42 °C) and high light treatment (1500 μmol m-2 s-1). These results suggest that XVSAP1 may have a significant role to play in the response of X. viscosa to abiotic stresses.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/54.381.191</identifier><identifier>PMID: 12493847</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>Oxford: OXFORD UNIVERSITY PRESS</publisher><subject>Amino Acid Sequence ; Amino acids ; Animal and plant ecology ; Animal, plant and microbial ecology ; Autoecology ; Base Sequence ; Biological and medical sciences ; Blotting, Southern ; Cell membranes ; Complementary DNA ; Dehydration ; DNA ; DNA, Complementary ; DNA, Plant ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Plant ; Genes. Genome ; Magnoliopsida - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Oral rehydration ; Osmotic Pressure ; Plant physiology ; Plant Proteins - genetics ; Plants ; Plants and fungi ; Recombinant Proteins ; Research Papers: Cell and Molecular Biology, Biochemistry and Molecular Physiology ; Resurrection ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid</subject><ispartof>Journal of experimental botany, 2003-01, Vol.54 (381), p.191-201</ispartof><rights>Society for Experimental Biology 2003</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-34e94e8b4e83c0f8e090f0a002bb81d46e79e8fded4508c6b68f3de01f8fc7173</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23697744$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23697744$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14429811$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12493847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garwe, Dahlia</creatorcontrib><creatorcontrib>Thomson, Jennifer A.</creatorcontrib><creatorcontrib>Mundree, Sagadevan G.</creatorcontrib><title>Molecular characterization of XVSAP1, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker</title><title>Journal of experimental botany</title><addtitle>J Exp Bot</addtitle><description>The strategy of 'complementation by functional sufficiency' was used to isolate a cDNA designated XVSAP1 from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low-temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana. Expression of XVSAP1 in Escherichia coli (srl::Tn10) conferred osmotic stress tolerance when the cells were grown in 1 M sorbitol. Analysis of gene expression using semi-quantitative RT-PCR indicated that XVSAP1 is induced by dehydration, salt stress (100 mM), both low (4 °C) and high temperature (42 °C) and high light treatment (1500 μmol m-2 s-1). These results suggest that XVSAP1 may have a significant role to play in the response of X. viscosa to abiotic stresses.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Autoecology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Cell membranes</subject><subject>Complementary DNA</subject><subject>Dehydration</subject><subject>DNA</subject><subject>DNA, Complementary</subject><subject>DNA, Plant</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Genes. Genome</subject><subject>Magnoliopsida - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Oral rehydration</subject><subject>Osmotic Pressure</subject><subject>Plant physiology</subject><subject>Plant Proteins - genetics</subject><subject>Plants</subject><subject>Plants and fungi</subject><subject>Recombinant Proteins</subject><subject>Research Papers: Cell and Molecular Biology, Biochemistry and Molecular Physiology</subject><subject>Resurrection</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Homology, Amino Acid</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1P3DAQxa2qqCxbrr2BfIETWcYfSewjIL4kUCu1RdxWjjNms83GwXYQ9K9vxG7hyGFmDu83T2-GkG8MZgy0OF4-V8e5nAnFZkyzT2TCZAEZl4J9JhMAzjPQeblNdmJcAkAOef6FbDMutVCynJBw61u0Q2sCtQsTjE0Ymr8mNb6j3tH7u58nP9gRNTSmgDFmY-t9F5snpA_YIXXBr2haIB2FIQS0r5t9a7pE7zH4fvGSDH1qovXR0FPzB8NXsuVMG3F3M6fk98X5r7Or7Ob75fXZyU1mhchTJiRqiaoaS1hwCkGDAzPeVFWK1bLAUqNyNdYyB2WLqlBO1AjMKWdLVoopOVz79sE_DhjTfDXGwHbMhn6I85IrlTPJPwSZUhzg1XF_Aw7VCut5H5qVCS_z_-8cgYMNYKI1rQums01856TkWjE2cntrbhmTD286F4UuSynFP6_-jys</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>Garwe, Dahlia</creator><creator>Thomson, Jennifer A.</creator><creator>Mundree, Sagadevan G.</creator><general>OXFORD UNIVERSITY PRESS</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030101</creationdate><title>Molecular characterization of XVSAP1, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker</title><author>Garwe, Dahlia ; Thomson, Jennifer A. ; Mundree, Sagadevan G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-34e94e8b4e83c0f8e090f0a002bb81d46e79e8fded4508c6b68f3de01f8fc7173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Autoecology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Cell membranes</topic><topic>Complementary DNA</topic><topic>Dehydration</topic><topic>DNA</topic><topic>DNA, Complementary</topic><topic>DNA, Plant</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Genes. Genome</topic><topic>Magnoliopsida - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Oral rehydration</topic><topic>Osmotic Pressure</topic><topic>Plant physiology</topic><topic>Plant Proteins - genetics</topic><topic>Plants</topic><topic>Plants and fungi</topic><topic>Recombinant Proteins</topic><topic>Research Papers: Cell and Molecular Biology, Biochemistry and Molecular Physiology</topic><topic>Resurrection</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garwe, Dahlia</creatorcontrib><creatorcontrib>Thomson, Jennifer A.</creatorcontrib><creatorcontrib>Mundree, Sagadevan G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garwe, Dahlia</au><au>Thomson, Jennifer A.</au><au>Mundree, Sagadevan G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of XVSAP1, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker</atitle><jtitle>Journal of experimental botany</jtitle><addtitle>J Exp Bot</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>54</volume><issue>381</issue><spage>191</spage><epage>201</epage><pages>191-201</pages><issn>0022-0957</issn><eissn>1460-2431</eissn><coden>JEBOA6</coden><abstract>The strategy of 'complementation by functional sufficiency' was used to isolate a cDNA designated XVSAP1 from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low-temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana. Expression of XVSAP1 in Escherichia coli (srl::Tn10) conferred osmotic stress tolerance when the cells were grown in 1 M sorbitol. Analysis of gene expression using semi-quantitative RT-PCR indicated that XVSAP1 is induced by dehydration, salt stress (100 mM), both low (4 °C) and high temperature (42 °C) and high light treatment (1500 μmol m-2 s-1). These results suggest that XVSAP1 may have a significant role to play in the response of X. viscosa to abiotic stresses.</abstract><cop>Oxford</cop><pub>OXFORD UNIVERSITY PRESS</pub><pmid>12493847</pmid><doi>10.1093/jxb/54.381.191</doi><tpages>11</tpages></addata></record> |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Amino acids Animal and plant ecology Animal, plant and microbial ecology Autoecology Base Sequence Biological and medical sciences Blotting, Southern Cell membranes Complementary DNA Dehydration DNA DNA, Complementary DNA, Plant Escherichia coli Fundamental and applied biological sciences. Psychology Genes Genes, Plant Genes. Genome Magnoliopsida - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Oral rehydration Osmotic Pressure Plant physiology Plant Proteins - genetics Plants Plants and fungi Recombinant Proteins Research Papers: Cell and Molecular Biology, Biochemistry and Molecular Physiology Resurrection Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid |
| title | Molecular characterization of XVSAP1, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker |
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