Antisense CCAAT/enhancer-binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 preadipocytes
Previous studies suggest that the CCAAT/enhancer-binding protein (C/EBP) functions in the coordinate expression of adipocyte genes during differentiation of 3T3-L1 preadipocytes. We sought to block expression of C/EBP selectively using a bovine papilloma virus (BPV) vector to direct transcription of...
Gespeichert in:
| Veröffentlicht in: | Genes & development 1992-04, Vol.6 (4), p.533-544 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 544 |
|---|---|
| container_issue | 4 |
| container_start_page | 533 |
| container_title | Genes & development |
| container_volume | 6 |
| creator | FANG-TSYR LIN LANE, M. D |
| description | Previous studies suggest that the CCAAT/enhancer-binding protein (C/EBP) functions in the coordinate expression of adipocyte genes during differentiation of 3T3-L1 preadipocytes. We sought to block expression of C/EBP selectively using a bovine papilloma virus (BPV) vector to direct transcription of a approximately 0.4-kb segment of C/EBP cDNA (in antisense orientation) containing translated sequence 5' to that encoding the basic and leucine zipper regions of the protein. Vector-directed expression of antisense C/EBP RNA in 3T3-L1 preadipocytes inhibited expression of C/EBP mRNA and protein, as well as several adipose-specific mRNAs, and also prevented cytoplasmic triglyceride accumulation. Rescue of the "adipocyte phenotype" was accomplished by transfection of cells expressing antisense RNA with a modified BPV vector that directs transcription of the complementary sense C/EBP RNA. |
| doi_str_mv | 10.1101/gad.6.4.533 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72884572</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72884572</sourcerecordid><originalsourceid>FETCH-LOGICAL-c447t-8b731e89abd475346cf05253821337116ae10afce54f7325e0eac5ac45dc65a03</originalsourceid><addsrcrecordid>eNqFkU2LFDEQhoMo6-zqybOQg3hZejbpfHUfm8EvGBRkPDc1SWWM9KTbpBt2fo1_1Ywz6NFTQb0Pb1E8hLzibM054w8HcGu9lmslxBOy4kq2lZLGPCUr1rSsaoVun5PbnH8wxjTT-obccGEE52ZFfnVxDhljRrrZdN3uAeN3iBZTtQ_RhXigUxpnDJF-_dzRvExTwpwxUzuOqeQwIz1gRIqPf5IwRgrR0TmFw3AqPcEhBWuX4zLAfE7dks61LniPCcv1y3r0VOxEteXlIIIL02hPM-YX5JmHIePL67wj396_220-VtsvHz5tum1lpTRz1ezLP9i0sHfSKCG19UzVSjQ1F8JwrgE5A29RSW9ErZAhWAVWKme1AibuyNtLb3n354J57o8hWxwGiDguuTd100hl6v-CXDfKqObceH8BbRpzTuj7KYUjpFPPWX_21hdvve5lX7wV-vW1dtkf0f1jL6JK_uaaQ7Yw-FQkhfwXU1zItmC_AcCAov0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16857580</pqid></control><display><type>article</type><title>Antisense CCAAT/enhancer-binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 preadipocytes</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>FANG-TSYR LIN ; LANE, M. D</creator><creatorcontrib>FANG-TSYR LIN ; LANE, M. D</creatorcontrib><description>Previous studies suggest that the CCAAT/enhancer-binding protein (C/EBP) functions in the coordinate expression of adipocyte genes during differentiation of 3T3-L1 preadipocytes. We sought to block expression of C/EBP selectively using a bovine papilloma virus (BPV) vector to direct transcription of a approximately 0.4-kb segment of C/EBP cDNA (in antisense orientation) containing translated sequence 5' to that encoding the basic and leucine zipper regions of the protein. Vector-directed expression of antisense C/EBP RNA in 3T3-L1 preadipocytes inhibited expression of C/EBP mRNA and protein, as well as several adipose-specific mRNAs, and also prevented cytoplasmic triglyceride accumulation. Rescue of the "adipocyte phenotype" was accomplished by transfection of cells expressing antisense RNA with a modified BPV vector that directs transcription of the complementary sense C/EBP RNA.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.6.4.533</identifier><identifier>PMID: 1373117</identifier><identifier>CODEN: GEDEEP</identifier><language>eng</language><publisher>Cold Spring Harbor, NY: Cold Spring Harbor Laboratory</publisher><subject>3T3 Cells ; Adipose Tissue - cytology ; Adipose Tissue - metabolism ; Animals ; Biological and medical sciences ; Blotting, Southern ; Blotting, Western ; CCAAT-Enhancer-Binding Proteins ; Cell Differentiation ; DNA - genetics ; DNA-Binding Proteins - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genetic Vectors ; Leucine Zippers - genetics ; Mice ; Molecular and cellular biology ; Molecular genetics ; Nuclear Proteins - genetics ; RNA - genetics ; Transcription, Genetic ; Transfection ; Triglycerides - metabolism</subject><ispartof>Genes & development, 1992-04, Vol.6 (4), p.533-544</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-8b731e89abd475346cf05253821337116ae10afce54f7325e0eac5ac45dc65a03</citedby><cites>FETCH-LOGICAL-c447t-8b731e89abd475346cf05253821337116ae10afce54f7325e0eac5ac45dc65a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5134917$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1373117$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FANG-TSYR LIN</creatorcontrib><creatorcontrib>LANE, M. D</creatorcontrib><title>Antisense CCAAT/enhancer-binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 preadipocytes</title><title>Genes & development</title><addtitle>Genes Dev</addtitle><description>Previous studies suggest that the CCAAT/enhancer-binding protein (C/EBP) functions in the coordinate expression of adipocyte genes during differentiation of 3T3-L1 preadipocytes. We sought to block expression of C/EBP selectively using a bovine papilloma virus (BPV) vector to direct transcription of a approximately 0.4-kb segment of C/EBP cDNA (in antisense orientation) containing translated sequence 5' to that encoding the basic and leucine zipper regions of the protein. Vector-directed expression of antisense C/EBP RNA in 3T3-L1 preadipocytes inhibited expression of C/EBP mRNA and protein, as well as several adipose-specific mRNAs, and also prevented cytoplasmic triglyceride accumulation. Rescue of the "adipocyte phenotype" was accomplished by transfection of cells expressing antisense RNA with a modified BPV vector that directs transcription of the complementary sense C/EBP RNA.</description><subject>3T3 Cells</subject><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>CCAAT-Enhancer-Binding Proteins</subject><subject>Cell Differentiation</subject><subject>DNA - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genetic Vectors</subject><subject>Leucine Zippers - genetics</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Nuclear Proteins - genetics</subject><subject>RNA - genetics</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><subject>Triglycerides - metabolism</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo6-zqybOQg3hZejbpfHUfm8EvGBRkPDc1SWWM9KTbpBt2fo1_1Ywz6NFTQb0Pb1E8hLzibM054w8HcGu9lmslxBOy4kq2lZLGPCUr1rSsaoVun5PbnH8wxjTT-obccGEE52ZFfnVxDhljRrrZdN3uAeN3iBZTtQ_RhXigUxpnDJF-_dzRvExTwpwxUzuOqeQwIz1gRIqPf5IwRgrR0TmFw3AqPcEhBWuX4zLAfE7dks61LniPCcv1y3r0VOxEteXlIIIL02hPM-YX5JmHIePL67wj396_220-VtsvHz5tum1lpTRz1ezLP9i0sHfSKCG19UzVSjQ1F8JwrgE5A29RSW9ErZAhWAVWKme1AibuyNtLb3n354J57o8hWxwGiDguuTd100hl6v-CXDfKqObceH8BbRpzTuj7KYUjpFPPWX_21hdvve5lX7wV-vW1dtkf0f1jL6JK_uaaQ7Yw-FQkhfwXU1zItmC_AcCAov0</recordid><startdate>19920401</startdate><enddate>19920401</enddate><creator>FANG-TSYR LIN</creator><creator>LANE, M. D</creator><general>Cold Spring Harbor Laboratory</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19920401</creationdate><title>Antisense CCAAT/enhancer-binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 preadipocytes</title><author>FANG-TSYR LIN ; LANE, M. D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-8b731e89abd475346cf05253821337116ae10afce54f7325e0eac5ac45dc65a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>3T3 Cells</topic><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>CCAAT-Enhancer-Binding Proteins</topic><topic>Cell Differentiation</topic><topic>DNA - genetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genetic Vectors</topic><topic>Leucine Zippers - genetics</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nuclear Proteins - genetics</topic><topic>RNA - genetics</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><topic>Triglycerides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FANG-TSYR LIN</creatorcontrib><creatorcontrib>LANE, M. D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genes & development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FANG-TSYR LIN</au><au>LANE, M. D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antisense CCAAT/enhancer-binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 preadipocytes</atitle><jtitle>Genes & development</jtitle><addtitle>Genes Dev</addtitle><date>1992-04-01</date><risdate>1992</risdate><volume>6</volume><issue>4</issue><spage>533</spage><epage>544</epage><pages>533-544</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><coden>GEDEEP</coden><abstract>Previous studies suggest that the CCAAT/enhancer-binding protein (C/EBP) functions in the coordinate expression of adipocyte genes during differentiation of 3T3-L1 preadipocytes. We sought to block expression of C/EBP selectively using a bovine papilloma virus (BPV) vector to direct transcription of a approximately 0.4-kb segment of C/EBP cDNA (in antisense orientation) containing translated sequence 5' to that encoding the basic and leucine zipper regions of the protein. Vector-directed expression of antisense C/EBP RNA in 3T3-L1 preadipocytes inhibited expression of C/EBP mRNA and protein, as well as several adipose-specific mRNAs, and also prevented cytoplasmic triglyceride accumulation. Rescue of the "adipocyte phenotype" was accomplished by transfection of cells expressing antisense RNA with a modified BPV vector that directs transcription of the complementary sense C/EBP RNA.</abstract><cop>Cold Spring Harbor, NY</cop><pub>Cold Spring Harbor Laboratory</pub><pmid>1373117</pmid><doi>10.1101/gad.6.4.533</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0890-9369 |
| ispartof | Genes & development, 1992-04, Vol.6 (4), p.533-544 |
| issn | 0890-9369 1549-5477 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72884572 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 3T3 Cells Adipose Tissue - cytology Adipose Tissue - metabolism Animals Biological and medical sciences Blotting, Southern Blotting, Western CCAAT-Enhancer-Binding Proteins Cell Differentiation DNA - genetics DNA-Binding Proteins - genetics Fundamental and applied biological sciences. Psychology Gene Expression Genetic Vectors Leucine Zippers - genetics Mice Molecular and cellular biology Molecular genetics Nuclear Proteins - genetics RNA - genetics Transcription, Genetic Transfection Triglycerides - metabolism |
| title | Antisense CCAAT/enhancer-binding protein RNA suppresses coordinate gene expression and triglyceride accumulation during differentiation of 3T3-L1 preadipocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T22%3A03%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antisense%20CCAAT/enhancer-binding%20protein%20RNA%20suppresses%20coordinate%20gene%20expression%20and%20triglyceride%20accumulation%20during%20differentiation%20of%203T3-L1%20preadipocytes&rft.jtitle=Genes%20&%20development&rft.au=FANG-TSYR%20LIN&rft.date=1992-04-01&rft.volume=6&rft.issue=4&rft.spage=533&rft.epage=544&rft.pages=533-544&rft.issn=0890-9369&rft.eissn=1549-5477&rft.coden=GEDEEP&rft_id=info:doi/10.1101/gad.6.4.533&rft_dat=%3Cproquest_cross%3E72884572%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16857580&rft_id=info:pmid/1373117&rfr_iscdi=true |