The glial cell undergoes apoptosis in the microchaete lineage of Drosophila

Apoptosis plays a major role in vertebrate and invertebrate development. The adult Drosophila thoracic microchaete is a mechanosensory organ whose development has been extensively studied as a model of how cell division and cell determination intermingle. This sensory organ arises from a cell lineag...

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Veröffentlicht in:Development (Cambridge) 2003-01, Vol.130 (1), p.123-133
Hauptverfasser: Fichelson, Pierre, Gho, Michel
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Gho, Michel
description Apoptosis plays a major role in vertebrate and invertebrate development. The adult Drosophila thoracic microchaete is a mechanosensory organ whose development has been extensively studied as a model of how cell division and cell determination intermingle. This sensory organ arises from a cell lineage that produces a glial cell and four other cells that form the organ. In this study, using an in vivo approach as well as fixed material, we show that the glial cell undergoes nucleus fragmentation shortly after birth. Fragmentation was blocked after overexpression of the caspase inhibitor p35 or removal of the pro-apoptotic genes reaper, hid and grim , showing that the glial cell undergoes apoptosis. Moreover, it seems that fragments are eliminated from the epithelium by mobile macrophages. Forcing survival of the glial cells induces precocious axonal outgrowth but does not affect final axonal patterning and connectivity. However, under these conditions, glial cells do not fragment but leave the epithelium by a mechanism that is reminiscent of cell competition. Finally, we present evidences showing that glial cells are committed to apoptosis independently of gcm and prospero expression. We suggest that apoptosis is triggered by a cell autonomous mechanism.
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The adult Drosophila thoracic microchaete is a mechanosensory organ whose development has been extensively studied as a model of how cell division and cell determination intermingle. This sensory organ arises from a cell lineage that produces a glial cell and four other cells that form the organ. In this study, using an in vivo approach as well as fixed material, we show that the glial cell undergoes nucleus fragmentation shortly after birth. Fragmentation was blocked after overexpression of the caspase inhibitor p35 or removal of the pro-apoptotic genes reaper, hid and grim , showing that the glial cell undergoes apoptosis. Moreover, it seems that fragments are eliminated from the epithelium by mobile macrophages. Forcing survival of the glial cells induces precocious axonal outgrowth but does not affect final axonal patterning and connectivity. 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subjects Animals
Animals, Genetically Modified
Animals, Newborn
Apoptosis - physiology
Axons - physiology
Cell Lineage
Cell Survival
Cysteine Proteinase Inhibitors - genetics
DNA-Binding Proteins
Drosophila - genetics
Drosophila - growth & development
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Epithelial Cells
Epithelium - growth & development
Gene Expression Regulation, Developmental
Inhibitor of Apoptosis Proteins
Microscopy, Confocal - methods
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neuroglia - cytology
Neuropeptides - genetics
Neuropeptides - metabolism
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Phagocytosis - physiology
Pupa
Sense Organs - cytology
Sense Organs - growth & development
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription Factors
Viral Proteins - genetics
title The glial cell undergoes apoptosis in the microchaete lineage of Drosophila
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