The glial cell undergoes apoptosis in the microchaete lineage of Drosophila
Apoptosis plays a major role in vertebrate and invertebrate development. The adult Drosophila thoracic microchaete is a mechanosensory organ whose development has been extensively studied as a model of how cell division and cell determination intermingle. This sensory organ arises from a cell lineag...
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Veröffentlicht in: | Development (Cambridge) 2003-01, Vol.130 (1), p.123-133 |
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description | Apoptosis plays a major role in vertebrate and invertebrate development. The adult Drosophila thoracic microchaete is a mechanosensory organ whose development has been extensively studied as a model of how cell division and cell determination intermingle. This sensory organ arises from a cell lineage that produces a glial cell and four other cells that form the organ. In this study, using an in vivo approach as well as fixed material, we show that the glial cell undergoes nucleus fragmentation shortly after birth. Fragmentation was blocked after overexpression of the caspase inhibitor p35 or removal of the pro-apoptotic genes reaper, hid and grim , showing that the glial cell undergoes apoptosis. Moreover, it seems that fragments are eliminated from the epithelium by mobile macrophages. Forcing survival of the glial cells induces precocious axonal outgrowth but does not affect final axonal patterning and connectivity. However, under these conditions, glial cells do not fragment but leave the epithelium by a mechanism that is reminiscent of cell competition. Finally, we present evidences showing that glial cells are committed to apoptosis independently of gcm and prospero expression. We suggest that apoptosis is triggered by a cell autonomous mechanism. |
doi_str_mv | 10.1242/dev.00198 |
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The adult Drosophila thoracic microchaete is a mechanosensory organ whose development has been extensively studied as a model of how cell division and cell determination intermingle. This sensory organ arises from a cell lineage that produces a glial cell and four other cells that form the organ. In this study, using an in vivo approach as well as fixed material, we show that the glial cell undergoes nucleus fragmentation shortly after birth. Fragmentation was blocked after overexpression of the caspase inhibitor p35 or removal of the pro-apoptotic genes reaper, hid and grim , showing that the glial cell undergoes apoptosis. Moreover, it seems that fragments are eliminated from the epithelium by mobile macrophages. Forcing survival of the glial cells induces precocious axonal outgrowth but does not affect final axonal patterning and connectivity. However, under these conditions, glial cells do not fragment but leave the epithelium by a mechanism that is reminiscent of cell competition. Finally, we present evidences showing that glial cells are committed to apoptosis independently of gcm and prospero expression. 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The adult Drosophila thoracic microchaete is a mechanosensory organ whose development has been extensively studied as a model of how cell division and cell determination intermingle. This sensory organ arises from a cell lineage that produces a glial cell and four other cells that form the organ. In this study, using an in vivo approach as well as fixed material, we show that the glial cell undergoes nucleus fragmentation shortly after birth. Fragmentation was blocked after overexpression of the caspase inhibitor p35 or removal of the pro-apoptotic genes reaper, hid and grim , showing that the glial cell undergoes apoptosis. Moreover, it seems that fragments are eliminated from the epithelium by mobile macrophages. Forcing survival of the glial cells induces precocious axonal outgrowth but does not affect final axonal patterning and connectivity. However, under these conditions, glial cells do not fragment but leave the epithelium by a mechanism that is reminiscent of cell competition. Finally, we present evidences showing that glial cells are committed to apoptosis independently of gcm and prospero expression. We suggest that apoptosis is triggered by a cell autonomous mechanism.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Animals, Newborn</subject><subject>Apoptosis - physiology</subject><subject>Axons - physiology</subject><subject>Cell Lineage</subject><subject>Cell Survival</subject><subject>Cysteine Proteinase Inhibitors - genetics</subject><subject>DNA-Binding Proteins</subject><subject>Drosophila - genetics</subject><subject>Drosophila - growth & development</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>Epithelial Cells</subject><subject>Epithelium - growth & development</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Inhibitor of Apoptosis Proteins</subject><subject>Microscopy, Confocal - methods</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neuroglia - cytology</subject><subject>Neuropeptides - genetics</subject><subject>Neuropeptides - metabolism</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Phagocytosis - physiology</subject><subject>Pupa</subject><subject>Sense Organs - cytology</subject><subject>Sense Organs - growth & development</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - metabolism</subject><subject>Transcription Factors</subject><subject>Viral Proteins - genetics</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAUhS0EouUx8AeQJyQkAn7FTkbEWyCxlNlynevEyK1DnIL497i0EiPTXb7z6dyD0Akll5QJdtXA5yUhtK520JQKpYqasnoXTUldkoLWNZ2gg5TeCSFcKrWPJjklMqKm6HnWAW6DNwFbCAGvlg0MbYSETR_7MSafsF_iMVMLb4doOwMj4OCXYFrA0eHbIabYdz6YI7TnTEhwvL2H6O3-bnbzWLy8PjzdXL8Ulot6LAQ4zksjGyhL6ZhQhucyDoyUwkgmQArnmrkFx0jJa2lsyUtrLFTGzlXF-CE623j7IX6sII164dO6vVlCXCWtWFUxwem_IFVElFKsjecbMH-Y0gBO94NfmOFbU6LXE-s8sf6dOLOnW-lqvoDmj9xumoGLDdD5tvvyA-i5jyG2Po1p7YEQe015FucM5z_t8obw</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>Fichelson, Pierre</creator><creator>Gho, Michel</creator><general>The Company of Biologists Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030101</creationdate><title>The glial cell undergoes apoptosis in the microchaete lineage of Drosophila</title><author>Fichelson, Pierre ; Gho, Michel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-4ef335a6de556f247a3412fea664a624e64ffdbcef205396ac535cace8acb7823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Animals, Newborn</topic><topic>Apoptosis - physiology</topic><topic>Axons - physiology</topic><topic>Cell Lineage</topic><topic>Cell Survival</topic><topic>Cysteine Proteinase Inhibitors - genetics</topic><topic>DNA-Binding Proteins</topic><topic>Drosophila - genetics</topic><topic>Drosophila - growth & development</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>Epithelial Cells</topic><topic>Epithelium - growth & development</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Inhibitor of Apoptosis Proteins</topic><topic>Microscopy, Confocal - methods</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neuroglia - cytology</topic><topic>Neuropeptides - genetics</topic><topic>Neuropeptides - metabolism</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Phagocytosis - physiology</topic><topic>Pupa</topic><topic>Sense Organs - cytology</topic><topic>Sense Organs - growth & development</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - metabolism</topic><topic>Transcription Factors</topic><topic>Viral Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fichelson, Pierre</creatorcontrib><creatorcontrib>Gho, Michel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fichelson, Pierre</au><au>Gho, Michel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The glial cell undergoes apoptosis in the microchaete lineage of Drosophila</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>130</volume><issue>1</issue><spage>123</spage><epage>133</epage><pages>123-133</pages><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>Apoptosis plays a major role in vertebrate and invertebrate development. The adult Drosophila thoracic microchaete is a mechanosensory organ whose development has been extensively studied as a model of how cell division and cell determination intermingle. This sensory organ arises from a cell lineage that produces a glial cell and four other cells that form the organ. In this study, using an in vivo approach as well as fixed material, we show that the glial cell undergoes nucleus fragmentation shortly after birth. Fragmentation was blocked after overexpression of the caspase inhibitor p35 or removal of the pro-apoptotic genes reaper, hid and grim , showing that the glial cell undergoes apoptosis. Moreover, it seems that fragments are eliminated from the epithelium by mobile macrophages. Forcing survival of the glial cells induces precocious axonal outgrowth but does not affect final axonal patterning and connectivity. However, under these conditions, glial cells do not fragment but leave the epithelium by a mechanism that is reminiscent of cell competition. Finally, we present evidences showing that glial cells are committed to apoptosis independently of gcm and prospero expression. We suggest that apoptosis is triggered by a cell autonomous mechanism.</abstract><cop>England</cop><pub>The Company of Biologists Limited</pub><pmid>12441297</pmid><doi>10.1242/dev.00198</doi><tpages>11</tpages></addata></record> |
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subjects | Animals Animals, Genetically Modified Animals, Newborn Apoptosis - physiology Axons - physiology Cell Lineage Cell Survival Cysteine Proteinase Inhibitors - genetics DNA-Binding Proteins Drosophila - genetics Drosophila - growth & development Drosophila Proteins - genetics Drosophila Proteins - metabolism Epithelial Cells Epithelium - growth & development Gene Expression Regulation, Developmental Inhibitor of Apoptosis Proteins Microscopy, Confocal - methods Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neuroglia - cytology Neuropeptides - genetics Neuropeptides - metabolism Nuclear Proteins - genetics Nuclear Proteins - metabolism Phagocytosis - physiology Pupa Sense Organs - cytology Sense Organs - growth & development Trans-Activators - genetics Trans-Activators - metabolism Transcription Factors Viral Proteins - genetics |
title | The glial cell undergoes apoptosis in the microchaete lineage of Drosophila |
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