GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha
The GSH2 gene, encoding Hansenula polymorpha γ-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3-kb XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and...
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| Veröffentlicht in: | FEMS yeast research 2002-08, Vol.2 (3), p.327-332 |
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| container_title | FEMS yeast research |
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| creator | Ubiyvovk, Vira M Nazarko, Taras Y Stasyk, Olena G Sohn, Min Jeong Kang, Hyun Ah Sibirny, Andrei A |
| description | The
GSH2 gene, encoding
Hansenula polymorpha γ-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient
gsh2 mutant of
H. polymorpha. The gene was isolated as a 4.3-kb
XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into
gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the
Candida albicans homologue (Gcs1p). In comparison to the
Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The
GSH2 sequence appears in the GenBank database under accession No. AF435121. |
| doi_str_mv | 10.1016/S1567-1356(02)00111-3 |
| format | Article |
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GSH2 gene, encoding
Hansenula polymorpha γ-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient
gsh2 mutant of
H. polymorpha. The gene was isolated as a 4.3-kb
XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into
gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the
Candida albicans homologue (Gcs1p). In comparison to the
Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The
GSH2 sequence appears in the GenBank database under accession No. AF435121.</description><identifier>ISSN: 1567-1356</identifier><identifier>EISSN: 1567-1364</identifier><identifier>DOI: 10.1016/S1567-1356(02)00111-3</identifier><identifier>PMID: 12702282</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Blotting, Southern ; Cloning, Molecular ; Genes, Fungal ; Genetic Complementation Test ; Glutathione - biosynthesis ; Glutathione - deficiency ; Glutathione Synthase - genetics ; Glutathione Synthase - metabolism ; Hansenula polymorpha ; Methanol - metabolism ; Methylotrophic yeast ; Molecular Sequence Data ; Mutation ; Pichia - enzymology ; Pichia - genetics ; Pichia - growth & development ; Sequence Alignment ; γ-Glutamylcysteine synthetase</subject><ispartof>FEMS yeast research, 2002-08, Vol.2 (3), p.327-332</ispartof><rights>2002 Federation of European Microbiological Societies</rights><rights>2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12702282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ubiyvovk, Vira M</creatorcontrib><creatorcontrib>Nazarko, Taras Y</creatorcontrib><creatorcontrib>Stasyk, Olena G</creatorcontrib><creatorcontrib>Sohn, Min Jeong</creatorcontrib><creatorcontrib>Kang, Hyun Ah</creatorcontrib><creatorcontrib>Sibirny, Andrei A</creatorcontrib><title>GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha</title><title>FEMS yeast research</title><addtitle>FEMS Yeast Res</addtitle><description>The
GSH2 gene, encoding
Hansenula polymorpha γ-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient
gsh2 mutant of
H. polymorpha. The gene was isolated as a 4.3-kb
XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into
gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the
Candida albicans homologue (Gcs1p). In comparison to the
Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The
GSH2 sequence appears in the GenBank database under accession No. AF435121.</description><subject>Amino Acid Sequence</subject><subject>Blotting, Southern</subject><subject>Cloning, Molecular</subject><subject>Genes, Fungal</subject><subject>Genetic Complementation Test</subject><subject>Glutathione - biosynthesis</subject><subject>Glutathione - deficiency</subject><subject>Glutathione Synthase - genetics</subject><subject>Glutathione Synthase - metabolism</subject><subject>Hansenula polymorpha</subject><subject>Methanol - metabolism</subject><subject>Methylotrophic yeast</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Pichia - enzymology</subject><subject>Pichia - genetics</subject><subject>Pichia - growth & development</subject><subject>Sequence Alignment</subject><subject>γ-Glutamylcysteine synthetase</subject><issn>1567-1356</issn><issn>1567-1364</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM9KxDAQh4Mo_n8EJSdRsJpMTNo9iSzqCoIH9RxiOtmNtE1tUqHP5Xv4TFZ2VTzNMPPND-Yj5ICzM864On_kUuUZF1IdMzhhjHOeiTWyvRqri_XfXqotshPj6wjljBWbZItDzgAK2CZ4-ziDU2roHBuk2NhQ-mZOPz-yedUnUw-VHWJCPy7j0KQFJhOR-oaOLa0xLYYqpC60C2_pgCYmOjNNxKavDG1DNdShaxdmj2w4U0XcX9Vd8nxz_TSdZfcPt3fTq_sMgU9SBo5ZZyYKhVMuVwBy4hy3CqQspGNSgsmdAmUK5OJFCuWsynkprLBKWCbELjla5rZdeOsxJl37aLGqTIOhjzqHomATgBE8XIH9S42lbjtfm27QP2JGgC2B0Ld_W6a_3es_95qBXrkfTy6XJzh--O6x09H60SiWvkObdBn8_wT9L0EL8QWUg4k_</recordid><startdate>200208</startdate><enddate>200208</enddate><creator>Ubiyvovk, Vira M</creator><creator>Nazarko, Taras Y</creator><creator>Stasyk, Olena G</creator><creator>Sohn, Min Jeong</creator><creator>Kang, Hyun Ah</creator><creator>Sibirny, Andrei A</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200208</creationdate><title>GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha</title><author>Ubiyvovk, Vira M ; Nazarko, Taras Y ; Stasyk, Olena G ; Sohn, Min Jeong ; Kang, Hyun Ah ; Sibirny, Andrei A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e219t-2f0cfa96e3f6f762259ff1c625585f0552a7f626a8e13b536fc671d3c3c63c033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Blotting, Southern</topic><topic>Cloning, Molecular</topic><topic>Genes, Fungal</topic><topic>Genetic Complementation Test</topic><topic>Glutathione - biosynthesis</topic><topic>Glutathione - deficiency</topic><topic>Glutathione Synthase - genetics</topic><topic>Glutathione Synthase - metabolism</topic><topic>Hansenula polymorpha</topic><topic>Methanol - metabolism</topic><topic>Methylotrophic yeast</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Pichia - enzymology</topic><topic>Pichia - genetics</topic><topic>Pichia - growth & development</topic><topic>Sequence Alignment</topic><topic>γ-Glutamylcysteine synthetase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ubiyvovk, Vira M</creatorcontrib><creatorcontrib>Nazarko, Taras Y</creatorcontrib><creatorcontrib>Stasyk, Olena G</creatorcontrib><creatorcontrib>Sohn, Min Jeong</creatorcontrib><creatorcontrib>Kang, Hyun Ah</creatorcontrib><creatorcontrib>Sibirny, Andrei A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS yeast research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ubiyvovk, Vira M</au><au>Nazarko, Taras Y</au><au>Stasyk, Olena G</au><au>Sohn, Min Jeong</au><au>Kang, Hyun Ah</au><au>Sibirny, Andrei A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha</atitle><jtitle>FEMS yeast research</jtitle><addtitle>FEMS Yeast Res</addtitle><date>2002-08</date><risdate>2002</risdate><volume>2</volume><issue>3</issue><spage>327</spage><epage>332</epage><pages>327-332</pages><issn>1567-1356</issn><eissn>1567-1364</eissn><abstract>The
GSH2 gene, encoding
Hansenula polymorpha γ-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient
gsh2 mutant of
H. polymorpha. The gene was isolated as a 4.3-kb
XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into
gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the
Candida albicans homologue (Gcs1p). In comparison to the
Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The
GSH2 sequence appears in the GenBank database under accession No. AF435121.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>12702282</pmid><doi>10.1016/S1567-1356(02)00111-3</doi><tpages>6</tpages></addata></record> |
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| ispartof | FEMS yeast research, 2002-08, Vol.2 (3), p.327-332 |
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| source | MEDLINE; Access via Oxford University Press (Open Access Collection); Access via Wiley Online Library; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Blotting, Southern Cloning, Molecular Genes, Fungal Genetic Complementation Test Glutathione - biosynthesis Glutathione - deficiency Glutathione Synthase - genetics Glutathione Synthase - metabolism Hansenula polymorpha Methanol - metabolism Methylotrophic yeast Molecular Sequence Data Mutation Pichia - enzymology Pichia - genetics Pichia - growth & development Sequence Alignment γ-Glutamylcysteine synthetase |
| title | GSH2, a gene encoding γ-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha |
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