Calcium dependence of A-CAM function in human renal epithelium
Previously we have used immunocytochemistry to identify A‐CAM as a major cell adhesion molecule in human renal tubular epithelium. In this study, we demonstrate the calcium dependence of cell‐cell adhesion and A‐CAM function in cultured human renal epithelial cells. Separation of adjacent cells was...
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| Veröffentlicht in: | The Journal of pathology 1992-02, Vol.166 (2), p.163-169 |
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| container_title | The Journal of pathology |
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| creator | Biddlestone, L. R. Fleming, S. |
| description | Previously we have used immunocytochemistry to identify A‐CAM as a major cell adhesion molecule in human renal tubular epithelium. In this study, we demonstrate the calcium dependence of cell‐cell adhesion and A‐CAM function in cultured human renal epithelial cells. Separation of adjacent cells was seen within 1 min following sequestration of calcium from the culture medium with EDTA. This loss of intercellular adhesion in low calcium conditions was accompanied by the disappearance of A‐CAM immunoreactivity. The changes were reversible and on return to normal calcium concentrations, A‐CAM immunoreactivity reappeared and cell adhesion was re‐established. The time course of this recovery was slow, taking 2–3 h. These results demonstrate the calcium dependence of cell adhesion in cultured human renal epithelial cells and confirm the adhesive function of the immunoreactive A‐CAM molecule. |
| doi_str_mv | 10.1002/path.1711660213 |
| format | Article |
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These results demonstrate the calcium dependence of cell adhesion in cultured human renal epithelial cells and confirm the adhesive function of the immunoreactive A‐CAM molecule.</description><identifier>ISSN: 0022-3417</identifier><identifier>EISSN: 1096-9896</identifier><identifier>DOI: 10.1002/path.1711660213</identifier><identifier>PMID: 1560317</identifier><identifier>CODEN: JPTLAS</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>A-CAM ; A-cell adhesion molecule ; Antigens, CD ; Biological and medical sciences ; Cadherins ; calcium ; Calcium - metabolism ; cell adhesion ; Cell Adhesion - physiology ; Cell Adhesion Molecules - analysis ; Cell Adhesion Molecules - metabolism ; Cell Membrane - chemistry ; Cell Membrane - metabolism ; Cells, Cultured ; Epithelial Cells ; epithelium ; Epithelium - chemistry ; Epithelium - metabolism ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunohistochemistry ; Kidney ; Kidney - chemistry ; Kidney - cytology ; Kidney - metabolism ; Vertebrates: urinary system</subject><ispartof>The Journal of pathology, 1992-02, Vol.166 (2), p.163-169</ispartof><rights>Copyright © 1992 John Wiley & Sons, Ltd.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4423-243c4b319cfff0564053e23b5ea287a7af32906bd715836748c9a2ecf8890f1c3</citedby><cites>FETCH-LOGICAL-c4423-243c4b319cfff0564053e23b5ea287a7af32906bd715836748c9a2ecf8890f1c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpath.1711660213$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpath.1711660213$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5483839$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1560317$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Biddlestone, L. R.</creatorcontrib><creatorcontrib>Fleming, S.</creatorcontrib><title>Calcium dependence of A-CAM function in human renal epithelium</title><title>The Journal of pathology</title><addtitle>J. Pathol</addtitle><description>Previously we have used immunocytochemistry to identify A‐CAM as a major cell adhesion molecule in human renal tubular epithelium. In this study, we demonstrate the calcium dependence of cell‐cell adhesion and A‐CAM function in cultured human renal epithelial cells. Separation of adjacent cells was seen within 1 min following sequestration of calcium from the culture medium with EDTA. This loss of intercellular adhesion in low calcium conditions was accompanied by the disappearance of A‐CAM immunoreactivity. The changes were reversible and on return to normal calcium concentrations, A‐CAM immunoreactivity reappeared and cell adhesion was re‐established. The time course of this recovery was slow, taking 2–3 h. These results demonstrate the calcium dependence of cell adhesion in cultured human renal epithelial cells and confirm the adhesive function of the immunoreactive A‐CAM molecule.</description><subject>A-CAM</subject><subject>A-cell adhesion molecule</subject><subject>Antigens, CD</subject><subject>Biological and medical sciences</subject><subject>Cadherins</subject><subject>calcium</subject><subject>Calcium - metabolism</subject><subject>cell adhesion</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Adhesion Molecules - analysis</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell Membrane - chemistry</subject><subject>Cell Membrane - metabolism</subject><subject>Cells, Cultured</subject><subject>Epithelial Cells</subject><subject>epithelium</subject><subject>Epithelium - chemistry</subject><subject>Epithelium - metabolism</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kidney</subject><subject>Kidney - chemistry</subject><subject>Kidney - cytology</subject><subject>Kidney - metabolism</subject><subject>Vertebrates: urinary system</subject><issn>0022-3417</issn><issn>1096-9896</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EKkvhzAkpB8QtrcffFhLSagVdRAsciuBmeb1jrSFxQpwI-u9JlVUrTj3NYZ7nndFLyEugZ0ApO-_9eDgDDaAUZcAfkRVQq2prrHpMVjPBai5APyXPSvlJKbVWyhNyAlJRDnpF3m18E9LUVnvsMe8xB6y6WK3rzfqqilMOY-pylXJ1mFqfqwGzbyrs03jAZtaekyfRNwVfHOcp-fbh_fVmW19-ufi4WV_WQQjGayZ4EDsONsQYqVSCSo6M7yR6ZrTXPnJmqdrtNUjDlRYmWM8wRGMsjRD4KXmz5PZD93vCMro2lYBN4zN2U3GaGUMN5w-CoMBYwfQMni9gGLpSBoyuH1LrhxsH1N1W626rdffVzsarY_S0a3F_zy9dzvvXx70vwTdx8DmkcodJYbjhdsbeLtif1ODNQ1fd1_X19r8n6sVOZcS_d7YffjmluZbu--cL9-mHAnq1NU7xf8mun3Y</recordid><startdate>199202</startdate><enddate>199202</enddate><creator>Biddlestone, L. R.</creator><creator>Fleming, S.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199202</creationdate><title>Calcium dependence of A-CAM function in human renal epithelium</title><author>Biddlestone, L. R. ; Fleming, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4423-243c4b319cfff0564053e23b5ea287a7af32906bd715836748c9a2ecf8890f1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>A-CAM</topic><topic>A-cell adhesion molecule</topic><topic>Antigens, CD</topic><topic>Biological and medical sciences</topic><topic>Cadherins</topic><topic>calcium</topic><topic>Calcium - metabolism</topic><topic>cell adhesion</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Adhesion Molecules - analysis</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell Membrane - chemistry</topic><topic>Cell Membrane - metabolism</topic><topic>Cells, Cultured</topic><topic>Epithelial Cells</topic><topic>epithelium</topic><topic>Epithelium - chemistry</topic><topic>Epithelium - metabolism</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Kidney</topic><topic>Kidney - chemistry</topic><topic>Kidney - cytology</topic><topic>Kidney - metabolism</topic><topic>Vertebrates: urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Biddlestone, L. R.</creatorcontrib><creatorcontrib>Fleming, S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Biddlestone, L. R.</au><au>Fleming, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium dependence of A-CAM function in human renal epithelium</atitle><jtitle>The Journal of pathology</jtitle><addtitle>J. Pathol</addtitle><date>1992-02</date><risdate>1992</risdate><volume>166</volume><issue>2</issue><spage>163</spage><epage>169</epage><pages>163-169</pages><issn>0022-3417</issn><eissn>1096-9896</eissn><coden>JPTLAS</coden><abstract>Previously we have used immunocytochemistry to identify A‐CAM as a major cell adhesion molecule in human renal tubular epithelium. In this study, we demonstrate the calcium dependence of cell‐cell adhesion and A‐CAM function in cultured human renal epithelial cells. Separation of adjacent cells was seen within 1 min following sequestration of calcium from the culture medium with EDTA. This loss of intercellular adhesion in low calcium conditions was accompanied by the disappearance of A‐CAM immunoreactivity. The changes were reversible and on return to normal calcium concentrations, A‐CAM immunoreactivity reappeared and cell adhesion was re‐established. The time course of this recovery was slow, taking 2–3 h. These results demonstrate the calcium dependence of cell adhesion in cultured human renal epithelial cells and confirm the adhesive function of the immunoreactive A‐CAM molecule.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>1560317</pmid><doi>10.1002/path.1711660213</doi><tpages>7</tpages></addata></record> |
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| ispartof | The Journal of pathology, 1992-02, Vol.166 (2), p.163-169 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | A-CAM A-cell adhesion molecule Antigens, CD Biological and medical sciences Cadherins calcium Calcium - metabolism cell adhesion Cell Adhesion - physiology Cell Adhesion Molecules - analysis Cell Adhesion Molecules - metabolism Cell Membrane - chemistry Cell Membrane - metabolism Cells, Cultured Epithelial Cells epithelium Epithelium - chemistry Epithelium - metabolism Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Humans Immunohistochemistry Kidney Kidney - chemistry Kidney - cytology Kidney - metabolism Vertebrates: urinary system |
| title | Calcium dependence of A-CAM function in human renal epithelium |
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