The major allergen (parvalbumin) of codfish is encoded by at least two isotypic genes: cDNA cloning, expression and antibody binding of the recombinant allergens
The major allergen (parvalbumin) from cod, designated Allergen M Gad c 1, has been intensively studied both from the structural and immunological sides. In the present study, transcripts of two isotypic parvalbumin genes in Atlantic cod were identified and characterized. Subsequently, subfragments w...
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| Veröffentlicht in: | Molecular immunology 2003, Vol.39 (10), p.595-602 |
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| creator | Van Do, Thien Hordvik, Ivar Endresen, Curt Elsayed, Said |
| description | The major allergen (parvalbumin) from cod, designated Allergen M
Gad c 1, has been intensively studied both from the structural and immunological sides. In the present study, transcripts of two isotypic parvalbumin genes in Atlantic cod were identified and characterized. Subsequently, subfragments were inserted into the expression vector pET-19b, generating plasmids with coding capacity for complete parvalbumin polypeptides fused to an N-terminal his
10 tag. Most of the recombinant products were found in the soluble fraction of the expression host
Escherichia coli. The target proteins showed to react with polyclonal antibodies raised against Allergen M and demonstrated binding to specific IgE from 12 sera of patients allergic to cod in ELISA inhibition experiments. Sera with classes 4 and 5 CAP FEIA exhibited also strong binding to recombinant parvalbumins in immunoblots. |
| doi_str_mv | 10.1016/S0161-5890(02)00200-6 |
| format | Article |
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Gad c 1, has been intensively studied both from the structural and immunological sides. In the present study, transcripts of two isotypic parvalbumin genes in Atlantic cod were identified and characterized. Subsequently, subfragments were inserted into the expression vector pET-19b, generating plasmids with coding capacity for complete parvalbumin polypeptides fused to an N-terminal his
10 tag. Most of the recombinant products were found in the soluble fraction of the expression host
Escherichia coli. The target proteins showed to react with polyclonal antibodies raised against Allergen M and demonstrated binding to specific IgE from 12 sera of patients allergic to cod in ELISA inhibition experiments. Sera with classes 4 and 5 CAP FEIA exhibited also strong binding to recombinant parvalbumins in immunoblots.</description><identifier>ISSN: 0161-5890</identifier><identifier>EISSN: 1872-9142</identifier><identifier>DOI: 10.1016/S0161-5890(02)00200-6</identifier><identifier>PMID: 12431393</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Allergen M ; Allergens ; Amino Acid Sequence ; Animals ; Antibodies - immunology ; Base Sequence ; Cloning, Molecular ; Codfish ; DNA, Complementary ; Fish hypersensitivity ; Fish Products - adverse effects ; Fish Proteins - immunology ; Fishes - genetics ; Fishes - immunology ; Food Hypersensitivity - immunology ; Immunoblotting ; Molecular Sequence Data ; Parvalbumin ; Parvalbumins - genetics ; Parvalbumins - immunology ; Recombinant allergens ; Sequence Alignment ; Sequence Analysis, DNA</subject><ispartof>Molecular immunology, 2003, Vol.39 (10), p.595-602</ispartof><rights>2003 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-f94fa2521eec2b23d15863ed0f4b81e19d41b08affad757d82b12a309e892f9b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0161589002002006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12431393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van Do, Thien</creatorcontrib><creatorcontrib>Hordvik, Ivar</creatorcontrib><creatorcontrib>Endresen, Curt</creatorcontrib><creatorcontrib>Elsayed, Said</creatorcontrib><title>The major allergen (parvalbumin) of codfish is encoded by at least two isotypic genes: cDNA cloning, expression and antibody binding of the recombinant allergens</title><title>Molecular immunology</title><addtitle>Mol Immunol</addtitle><description>The major allergen (parvalbumin) from cod, designated Allergen M
Gad c 1, has been intensively studied both from the structural and immunological sides. In the present study, transcripts of two isotypic parvalbumin genes in Atlantic cod were identified and characterized. Subsequently, subfragments were inserted into the expression vector pET-19b, generating plasmids with coding capacity for complete parvalbumin polypeptides fused to an N-terminal his
10 tag. Most of the recombinant products were found in the soluble fraction of the expression host
Escherichia coli. The target proteins showed to react with polyclonal antibodies raised against Allergen M and demonstrated binding to specific IgE from 12 sera of patients allergic to cod in ELISA inhibition experiments. Sera with classes 4 and 5 CAP FEIA exhibited also strong binding to recombinant parvalbumins in immunoblots.</description><subject>Allergen M</subject><subject>Allergens</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>Codfish</subject><subject>DNA, Complementary</subject><subject>Fish hypersensitivity</subject><subject>Fish Products - adverse effects</subject><subject>Fish Proteins - immunology</subject><subject>Fishes - genetics</subject><subject>Fishes - immunology</subject><subject>Food Hypersensitivity - immunology</subject><subject>Immunoblotting</subject><subject>Molecular Sequence Data</subject><subject>Parvalbumin</subject><subject>Parvalbumins - genetics</subject><subject>Parvalbumins - immunology</subject><subject>Recombinant allergens</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><issn>0161-5890</issn><issn>1872-9142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuFDEQRS0EIpPAJ4C8QolEQ9n9stmgKOElRbAgrC0_yomj7nbH7gnM5_CneDKjsMzCtsp1qm6pLiGvGLxjwLr3P8vFqlZIOAZ-AsABqu4JWTHR80qyhj8lqwfkgBzmfAMAHXTtc3LAeFOzWtYr8vfyGumob2KiehgwXeFEj2ed7vRg1mOYTmj01EbnQ76mIVOcSoCOmg3VCx1Q54Uuv2NJxWUzB0tLA8wfqD3_fkrtEKcwXb2l-GdOmHOIE9WTK2cJJroNNWFyBdhqLGWOhDaO5a_kH6bJL8gzr4eML_fvEfn1-dPl2dfq4seXb2enF5VtWrFUXjZe85YzRMsNrx1rRVejA98YwZBJ1zADQnuvXd_2TnDDuK5BopDcS1MfkTe7vnOKt2vMixpDtjgMesK4zqrnQkAjxaMgK7oSelnAdgfaFHNO6NWcwqjTRjFQWxPVvYlq65ACru5NVF2pe70XWJsR3f-qvWsF-LgDsOzjLmBS2YbiDLpQVrgoF8MjEv8ANWmuPw</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Van Do, Thien</creator><creator>Hordvik, Ivar</creator><creator>Endresen, Curt</creator><creator>Elsayed, Said</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>The major allergen (parvalbumin) of codfish is encoded by at least two isotypic genes: cDNA cloning, expression and antibody binding of the recombinant allergens</title><author>Van Do, Thien ; Hordvik, Ivar ; Endresen, Curt ; Elsayed, Said</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-f94fa2521eec2b23d15863ed0f4b81e19d41b08affad757d82b12a309e892f9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Allergen M</topic><topic>Allergens</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>Codfish</topic><topic>DNA, Complementary</topic><topic>Fish hypersensitivity</topic><topic>Fish Products - adverse effects</topic><topic>Fish Proteins - immunology</topic><topic>Fishes - genetics</topic><topic>Fishes - immunology</topic><topic>Food Hypersensitivity - immunology</topic><topic>Immunoblotting</topic><topic>Molecular Sequence Data</topic><topic>Parvalbumin</topic><topic>Parvalbumins - genetics</topic><topic>Parvalbumins - immunology</topic><topic>Recombinant allergens</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van Do, Thien</creatorcontrib><creatorcontrib>Hordvik, Ivar</creatorcontrib><creatorcontrib>Endresen, Curt</creatorcontrib><creatorcontrib>Elsayed, Said</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van Do, Thien</au><au>Hordvik, Ivar</au><au>Endresen, Curt</au><au>Elsayed, Said</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The major allergen (parvalbumin) of codfish is encoded by at least two isotypic genes: cDNA cloning, expression and antibody binding of the recombinant allergens</atitle><jtitle>Molecular immunology</jtitle><addtitle>Mol Immunol</addtitle><date>2003</date><risdate>2003</risdate><volume>39</volume><issue>10</issue><spage>595</spage><epage>602</epage><pages>595-602</pages><issn>0161-5890</issn><eissn>1872-9142</eissn><abstract>The major allergen (parvalbumin) from cod, designated Allergen M
Gad c 1, has been intensively studied both from the structural and immunological sides. In the present study, transcripts of two isotypic parvalbumin genes in Atlantic cod were identified and characterized. Subsequently, subfragments were inserted into the expression vector pET-19b, generating plasmids with coding capacity for complete parvalbumin polypeptides fused to an N-terminal his
10 tag. Most of the recombinant products were found in the soluble fraction of the expression host
Escherichia coli. The target proteins showed to react with polyclonal antibodies raised against Allergen M and demonstrated binding to specific IgE from 12 sera of patients allergic to cod in ELISA inhibition experiments. Sera with classes 4 and 5 CAP FEIA exhibited also strong binding to recombinant parvalbumins in immunoblots.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>12431393</pmid><doi>10.1016/S0161-5890(02)00200-6</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular immunology, 2003, Vol.39 (10), p.595-602 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Allergen M Allergens Amino Acid Sequence Animals Antibodies - immunology Base Sequence Cloning, Molecular Codfish DNA, Complementary Fish hypersensitivity Fish Products - adverse effects Fish Proteins - immunology Fishes - genetics Fishes - immunology Food Hypersensitivity - immunology Immunoblotting Molecular Sequence Data Parvalbumin Parvalbumins - genetics Parvalbumins - immunology Recombinant allergens Sequence Alignment Sequence Analysis, DNA |
| title | The major allergen (parvalbumin) of codfish is encoded by at least two isotypic genes: cDNA cloning, expression and antibody binding of the recombinant allergens |
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